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. 1996 Oct;34(10):2391–2394. doi: 10.1128/jcm.34.10.2391-2394.1996

Comparison of fluorescent BACTEC 9000 MB system, Septi-Chek AFB system, and Lowenstein-Jensen medium for detection of mycobacteria.

A J van Griethuysen 1, A R Jansz 1, A G Buiting 1
PMCID: PMC229277  PMID: 8880486

Abstract

The newly developed fluorescent BACTEC 9000 MB system for automated culture of mycobacteria was compared with the Septi-Chek AFB system and Lowenstein-Jensen medium (LJ). A total of 2,005 clinical specimens were included in the study. Mycobacteria were isolated from 202 (10.1%) specimens, including 155 Mycobacterium tuberculosis complex isolates and 47 Mycobacteria other than M. tuberculosis isolates. Of 131 isolates detected by the BACTEC system, the Septi-Chek AFB system, or both, 120 (91.6%) were detected by the BACTEC system and 105 (80.2%) were detected by the Septi-Chek AFB system (P < 0.02). The recovery rate in the BACTEC system compared with that in the Septi-Chek AFB system was significantly higher for M. tuberculosis complex isolates (P < 0.005) and for isolates from acid-fast smear-negative specimens (P < 0.01). Of 148 isolates detected by the BACTEC system, LJ, or both, 142 (95.9%) were detected by the BACTEC system and 118 (79.9%) were detected by LJ (P < 0.001). The recovery rate in the BACTEC system compared with that on LJ was significantly higher for M. tuberculosis complex isolates (P < 0.001). The BACTEC system detected more mycobacteria from both smear-positive and smear-negative specimens than LJ. The mean times to detection of mycobacteria were 17.6 days for the BACTEC system, 26.0 days for the Septi-Chek AFB system, and 29.4 days for LJ. The BACTEC fluorescent 9000 MB system is a rapid, sensitive, and efficient method for the isolation of mycobacteria.

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Selected References

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  1. Abe C., Hosojima S., Fukasawa Y., Kazumi Y., Takahashi M., Hirano K., Mori T. Comparison of MB-Check, BACTEC, and egg-based media for recovery of mycobacteria. J Clin Microbiol. 1992 Apr;30(4):878–881. doi: 10.1128/jcm.30.4.878-881.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anargyros P., Astill D. S., Lim I. S. Comparison of improved BACTEC and Lowenstein-Jensen media for culture of mycobacteria from clinical specimens. J Clin Microbiol. 1990 Jun;28(6):1288–1291. doi: 10.1128/jcm.28.6.1288-1291.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. D'Amato R. F., Isenberg H. D., Hochstein L., Mastellone A. J., Alperstein P. Evaluation of the Roche Septi-Chek AFB system for recovery of mycobacteria. J Clin Microbiol. 1991 Dec;29(12):2906–2908. doi: 10.1128/jcm.29.12.2906-2908.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Giger T., Burkardt H. J. Evaluation of a new biphasic culture system for the recovery of mycobacteria. Eur J Clin Microbiol Infect Dis. 1990 Jun;9(6):428–431. doi: 10.1007/BF01979476. [DOI] [PubMed] [Google Scholar]
  5. Hoffner S. E., Haile M., Källenius G. A biphasic system for primary isolation of mycobacteria compared to solid medium and broth culture. J Med Microbiol. 1992 Nov;37(5):332–334. doi: 10.1099/00222615-37-5-332. [DOI] [PubMed] [Google Scholar]
  6. Hoffner S. E., Henriques B., Petrini B., Källenius G. Mycobacterium malmoense: an easily missed pathogen. J Clin Microbiol. 1991 Nov;29(11):2673–2674. doi: 10.1128/jcm.29.11.2673-2674.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hoffner S. E. Improved detection of Mycobacterium avium complex with the Bactec radiometric system. Diagn Microbiol Infect Dis. 1988 May;10(1):1–6. doi: 10.1016/0732-8893(88)90121-6. [DOI] [PubMed] [Google Scholar]
  8. Isenberg H. D., D'Amato R. F., Heifets L., Murray P. R., Scardamaglia M., Jacobs M. C., Alperstein P., Niles A. Collaborative feasibility study of a biphasic system (Roche Septi-Chek AFB) for rapid detection and isolation of mycobacteria. J Clin Microbiol. 1991 Aug;29(8):1719–1722. doi: 10.1128/jcm.29.8.1719-1722.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kaminski D. A., Hardy D. J. Selective utilization of DNA probes for identification of Mycobacterium species on the basis of cord formation in primary BACTEC 12B cultures. J Clin Microbiol. 1995 Jun;33(6):1548–1550. doi: 10.1128/jcm.33.6.1548-1550.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kirihara J. M., Hillier S. L., Coyle M. B. Improved detection times for Mycobacterium avium complex and Mycobacterium tuberculosis with the BACTEC radiometric system. J Clin Microbiol. 1985 Nov;22(5):841–845. doi: 10.1128/jcm.22.5.841-845.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Morgan M. A., Horstmeier C. D., DeYoung D. R., Roberts G. D. Comparison of a radiometric method (BACTEC) and conventional culture media for recovery of mycobacteria from smear-negative specimens. J Clin Microbiol. 1983 Aug;18(2):384–388. doi: 10.1128/jcm.18.2.384-388.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Morris A. J., Reller L. B. Reliability of cord formation in BACTEC media for presumptive identification of mycobacteria. J Clin Microbiol. 1993 Sep;31(9):2533–2534. doi: 10.1128/jcm.31.9.2533-2534.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Piersimoni C., Morbiducci V., De Sio G., Scalise G. Comparative evaluation of the MB-Check system for recovery of mycobacteria from clinical specimens. Eur J Clin Microbiol Infect Dis. 1992 Dec;11(12):1174–1177. doi: 10.1007/BF01961140. [DOI] [PubMed] [Google Scholar]
  14. Roberts G. D., Goodman N. L., Heifets L., Larsh H. W., Lindner T. H., McClatchy J. K., McGinnis M. R., Siddiqi S. H., Wright P. Evaluation of the BACTEC radiometric method for recovery of mycobacteria and drug susceptibility testing of Mycobacterium tuberculosis from acid-fast smear-positive specimens. J Clin Microbiol. 1983 Sep;18(3):689–696. doi: 10.1128/jcm.18.3.689-696.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sewell D. L., Rashad A. L., Rourke W. J., Jr, Poor S. L., McCarthy J. A., Pfaller M. A. Comparison of the Septi-Chek AFB and BACTEC systems and conventional culture for recovery of mycobacteria. J Clin Microbiol. 1993 Oct;31(10):2689–2691. doi: 10.1128/jcm.31.10.2689-2691.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Whittier P. S., Westfall K., Setterquist S., Hopfer R. L. Evaluation of the Septi-Chek AFB system in the recovery of mycobacteria. Eur J Clin Microbiol Infect Dis. 1992 Oct;11(10):915–918. doi: 10.1007/BF01962373. [DOI] [PubMed] [Google Scholar]
  17. Wilson M. L., Stone B. L., Hildred M. V., Reves R. R. Comparison of recovery rates for mycobacteria from BACTEC 12B vials, Middlebrook 7H11-selective 7H11 biplates, and Lowenstein Jensen slants in a public health mycobacteriology laboratory. J Clin Microbiol. 1995 Sep;33(9):2516–2518. doi: 10.1128/jcm.33.9.2516-2518.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Woods G. L., Witebsky F. G. Mycobacterial testing in clinical laboratories that participate in the College of American Pathologists' Mycobacteriology E survey: results of a 1993 questionnaire. J Clin Microbiol. 1995 Feb;33(2):407–412. doi: 10.1128/jcm.33.2.407-412.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yagupsky P. V., Kaminski D. A., Palmer K. M., Nolte F. S. Cord formation in BACTEC 7H12 medium for rapid, presumptive identification of Mycobacterium tuberculosis complex. J Clin Microbiol. 1990 Jun;28(6):1451–1453. doi: 10.1128/jcm.28.6.1451-1453.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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