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. 1996 Oct;34(10):2440–2443. doi: 10.1128/jcm.34.10.2440-2443.1996

PCR ribotyping for characterizing Salmonella isolates of different serotypes.

C Lagatolla 1, L Dolzani 1, E Tonin 1, A Lavenia 1, M Di Michele 1, T Tommasini 1, C Monti-Bragadin 1
PMCID: PMC229289  PMID: 8880496

Abstract

The 16S-23S intergenic spacer region in 218 strains of Salmonella isolated from four Italian hospitals during the period from 1977 to 1994 was analyzed by PCR ribotyping. This molecular typing technique allowed for the identification of seven different and specific electrophoretic profiles for the seven serovars S: enteritidis, S. london, S. anatum, S. panama, S. heidelberg, S. agona, and S. goldcoast. Otherwise, the spacer region appears to be polymorphic in S. typhimurium S. infantis, and S. derby since we could identify eight, six, and four different ribotypes, respectively.

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Selected References

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  1. Altwegg M., Hickman-Brenner F. W., Farmer J. J., 3rd Ribosomal RNA gene restriction patterns provide increased sensitivity for typing Salmonella typhi strains. J Infect Dis. 1989 Jul;160(1):145–149. doi: 10.1093/infdis/160.1.145. [DOI] [PubMed] [Google Scholar]
  2. Baquar N., Threlfall E. J., Rowe B., Stanley J. Molecular subtyping within a single Salmonella typhimurium phage type, DT204c, with a PCR-generated probe for IS200. FEMS Microbiol Lett. 1993 Sep 1;112(2):217–221. doi: 10.1111/j.1574-6968.1993.tb06451.x. [DOI] [PubMed] [Google Scholar]
  3. Baquar N., Threlfall E. J., Rowe B., Stanley J. Phage type 193 of Salmonella typhimurium contains different chromosomal genotypes and multiple IS200 profiles. FEMS Microbiol Lett. 1994 Jan 15;115(2-3):291–295. doi: 10.1111/j.1574-6968.1994.tb06653.x. [DOI] [PubMed] [Google Scholar]
  4. Beltran P., Musser J. M., Helmuth R., Farmer J. J., 3rd, Frerichs W. M., Wachsmuth I. K., Ferris K., McWhorter A. C., Wells J. G., Cravioto A. Toward a population genetic analysis of Salmonella: genetic diversity and relationships among strains of serotypes S. choleraesuis, S. derby, S. dublin, S. enteritidis, S. heidelberg, S. infantis, S. newport, and S. typhimurium. Proc Natl Acad Sci U S A. 1988 Oct;85(20):7753–7757. doi: 10.1073/pnas.85.20.7753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Boyd E. F., Wang F. S., Beltran P., Plock S. A., Nelson K., Selander R. K. Salmonella reference collection B (SARB): strains of 37 serovars of subspecies I. J Gen Microbiol. 1993 Jun;139(Pt 6):1125–1132. doi: 10.1099/00221287-139-6-1125. [DOI] [PubMed] [Google Scholar]
  6. Crosa J. H., Brenner D. J., Ewing W. H., Falkow S. Molecular relationships among the Salmonelleae. J Bacteriol. 1973 Jul;115(1):307–315. doi: 10.1128/jb.115.1.307-315.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Doll L., Moshitch S., Frankel G. Poly(GTG)5-associated profiles of Salmonella and Shigella genomic DNA. Res Microbiol. 1993 Jan;144(1):17–24. doi: 10.1016/0923-2508(93)90211-j. [DOI] [PubMed] [Google Scholar]
  8. Dolzani L., Tonin E., Lagatolla C., Monti-Bragadin C. Typing of Staphylococcus aureus by amplification of the 16S-23S rRNA intergenic spacer sequences. FEMS Microbiol Lett. 1994 Jun 1;119(1-2):167–173. doi: 10.1111/j.1574-6968.1994.tb06884.x. [DOI] [PubMed] [Google Scholar]
  9. Dolzani L., Tonin E., Lagatolla C., Prandin L., Monti-Bragadin C. Identification of Acinetobacter isolates in the A. calcoaceticus-A. baumannii complex by restriction analysis of the 16S-23S rRNA intergenic-spacer sequences. J Clin Microbiol. 1995 May;33(5):1108–1113. doi: 10.1128/jcm.33.5.1108-1113.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Esteban E., Snipes K., Hird D., Kasten R., Kinde H. Use of ribotyping for characterization of Salmonella serotypes. J Clin Microbiol. 1993 Feb;31(2):233–237. doi: 10.1128/jcm.31.2.233-237.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ezquerra E., Burnens A., Jones C., Stanley J. Genotypic typing and phylogenetic analysis of Salmonella paratyphi B and S. java with IS200. J Gen Microbiol. 1993 Oct;139(10):2409–2414. doi: 10.1099/00221287-139-10-2409. [DOI] [PubMed] [Google Scholar]
  12. Gruner E., Martinetti Lucchini G., Hoop R. K., Altwegg M. Molecular epidemiology of Salmonella enteritidis. Eur J Epidemiol. 1994 Feb;10(1):85–89. doi: 10.1007/BF01717458. [DOI] [PubMed] [Google Scholar]
  13. Jensen M. A., Webster J. A., Straus N. Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. Appl Environ Microbiol. 1993 Apr;59(4):945–952. doi: 10.1128/aem.59.4.945-952.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kostman J. R., Edlind T. D., LiPuma J. J., Stull T. L. Molecular epidemiology of Pseudomonas cepacia determined by polymerase chain reaction ribotyping. J Clin Microbiol. 1992 Aug;30(8):2084–2087. doi: 10.1128/jcm.30.8.2084-2087.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nastasi A., Mammina C. Epidemiological evaluation by PCR ribotyping of sporadic and outbreak-associated strains of Salmonella enterica serotype Typhimurium. Res Microbiol. 1995 Jan;146(1):99–106. doi: 10.1016/0923-2508(96)80274-9. [DOI] [PubMed] [Google Scholar]
  16. Nastasi A., Mammina C., Villafrate M. R. Epidemiology of Salmonella typhimurium: ribosomal DNA analysis of strains from human and animal sources. Epidemiol Infect. 1993 Jun;110(3):553–565. doi: 10.1017/s0950268800050974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Olsen J. E., Skov M. N., Threlfall E. J., Brown D. J. Clonal lines of Salmonella enterica serotype Enteritidis documented by IS200-, ribo-, pulsed-field gel electrophoresis and RFLP typing. J Med Microbiol. 1994 Jan;40(1):15–22. doi: 10.1099/00222615-40-1-15. [DOI] [PubMed] [Google Scholar]
  18. Selander R. K., Beltran P., Smith N. H., Helmuth R., Rubin F. A., Kopecko D. J., Ferris K., Tall B. D., Cravioto A., Musser J. M. Evolutionary genetic relationships of clones of Salmonella serovars that cause human typhoid and other enteric fevers. Infect Immun. 1990 Jul;58(7):2262–2275. doi: 10.1128/iai.58.7.2262-2275.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stanley J., Baquar N. Phylogenetics of Salmonella enteritidis. Int J Food Microbiol. 1994 Jan;21(1-2):79–87. doi: 10.1016/0168-1605(94)90202-x. [DOI] [PubMed] [Google Scholar]
  20. Stanley J., Baquar N., Threlfall E. J. Genotypes and phylogenetic relationships of Salmonella typhimurium are defined by molecular fingerprinting of IS200 and 16S rrn loci. J Gen Microbiol. 1993 Jun;139(Pt 6):1133–1140. doi: 10.1099/00221287-139-6-1133. [DOI] [PubMed] [Google Scholar]
  21. Stanley J., Burnens A., Powell N., Chowdry N., Jones C. The insertion sequence IS200 fingerprints chromosomal genotypes and epidemiological relationships in Salmonella heidelberg. J Gen Microbiol. 1992 Nov;138(11):2329–2336. doi: 10.1099/00221287-138-11-2329. [DOI] [PubMed] [Google Scholar]
  22. Stanley J., Chowdry N., Powell N., Threlfall E. J. Chromosomal genotypes (evolutionary lines) of Salmonella berta. FEMS Microbiol Lett. 1992 Aug 15;74(2-3):247–252. doi: 10.1016/0378-1097(92)90437-s. [DOI] [PubMed] [Google Scholar]
  23. Stanley J., Jones C. S., Threlfall E. J. Evolutionary lines among Salmonella enteritidis phage types are identified by insertion sequence IS200 distribution. FEMS Microbiol Lett. 1991 Jul 15;66(1):83–89. doi: 10.1016/0378-1097(91)90425-a. [DOI] [PubMed] [Google Scholar]
  24. Threlfall E. J., Frost J. A. The identification, typing and fingerprinting of Salmonella: laboratory aspects and epidemiological applications. J Appl Bacteriol. 1990 Jan;68(1):5–16. doi: 10.1111/j.1365-2672.1990.tb02542.x. [DOI] [PubMed] [Google Scholar]
  25. Threlfall E. J., Torre E., Ward L. R., Dávalos-Pérez A., Rowe B., Gibert I. Insertion sequence IS200 fingerprinting of Salmonella typhi: an assessment of epidemiological applicability. Epidemiol Infect. 1994 Apr;112(2):253–261. doi: 10.1017/s0950268800057666. [DOI] [PMC free article] [PubMed] [Google Scholar]

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