Abstract
A disadvantage of genotyping bacterial strains by pulsed-field gel electrophoresis is that the procedure requires up to 6 days to complete. We modified a standard pulsed-field gel electrophoresis method (B.E. Murray, K.V. Singh, J.D. Health, B.R. Sharma, and G.M. Weinstock, J.Clin. Microbiol. 28:2059-2063, 1990) so that it could be completed in less than 3 days. We successfully applied this method to the analysis of a variety of gram-positive and gram-negative bacteria.
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Selected References
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- Bannerman T. L., Hancock G. A., Tenover F. C., Miller J. M. Pulsed-field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus. J Clin Microbiol. 1995 Mar;33(3):551–555. doi: 10.1128/jcm.33.3.551-555.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goering R. V. Molecular epidemiology of nosocomial infection: analysis of chromosomal restriction fragment patterns by pulsed-field gel electrophoresis. Infect Control Hosp Epidemiol. 1993 Oct;14(10):595–600. doi: 10.1086/646645. [DOI] [PubMed] [Google Scholar]
- Goering R. V., Winters M. A. Rapid method for epidemiological evaluation of gram-positive cocci by field inversion gel electrophoresis. J Clin Microbiol. 1992 Mar;30(3):577–580. doi: 10.1128/jcm.30.3.577-580.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leonard R. B., Mayer J., Sasser M., Woods M. L., Mooney B. R., Brinton B. G., Newcomb-Gayman P. L., Carroll K. C. Comparison of MIDI Sherlock system and pulsed-field gel electrophoresis in characterizing strains of methicillin-resistant Staphylococcus aureus from a recent hospital outbreak. J Clin Microbiol. 1995 Oct;33(10):2723–2727. doi: 10.1128/jcm.33.10.2723-2727.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maslow J. N., Mulligan M. E., Arbeit R. D. Molecular epidemiology: application of contemporary techniques to the typing of microorganisms. Clin Infect Dis. 1993 Aug;17(2):153–164. doi: 10.1093/clinids/17.2.153. [DOI] [PubMed] [Google Scholar]
- Murray B. E., Singh K. V., Heath J. D., Sharma B. R., Weinstock G. M. Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites. J Clin Microbiol. 1990 Sep;28(9):2059–2063. doi: 10.1128/jcm.28.9.2059-2063.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tenover F. C., Arbeit R. D., Goering R. V., Mickelsen P. A., Murray B. E., Persing D. H., Swaminathan B. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol. 1995 Sep;33(9):2233–2239. doi: 10.1128/jcm.33.9.2233-2239.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]