Abstract
Selective amplification of a 187-bp fragment within the DT6 sequence using the AV6 and AV7 primers for Mycobacterium avium and of a 666-bp fragment within the DT1 sequence of Mycobacterium intracellulare using the IN38 and IN41 primers was performed for 69 clinical isolates identified as M. avium complex by conventional methods. The results were compared in parallel with results with commercial M. avium and M. intracellulare probes. A positive response to either of the two PCRs or M. avium-M. intracellulare AccuProbes constituted positive detection as M. avium complex; this cumulative detection limit was 94.2% for PCR, compared with 90% for AccuProbe. Concordance, on the other hand, was considered an identical species identification using either DT1 PCR and the M. intracellulare probe or DT6 and DT1 PCRs are inexpensive and at least equally sensitive, in-house options to the AccuProbe system for species identification of M. avium and M. intracellulare.
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- Baess I. Deoxyribonucleic acid relationships between different serovars of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium scrofulaceum. Acta Pathol Microbiol Immunol Scand B. 1983 Jun;91(3):201–203. doi: 10.1111/j.1699-0463.1983.tb00033.x. [DOI] [PubMed] [Google Scholar]
- Butler W. R., Kilburn J. O. Identification of major slowly growing pathogenic mycobacteria and Mycobacterium gordonae by high-performance liquid chromatography of their mycolic acids. J Clin Microbiol. 1988 Jan;26(1):50–53. doi: 10.1128/jcm.26.1.50-53.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Böddinghaus B., Rogall T., Flohr T., Blöcker H., Böttger E. C. Detection and identification of mycobacteria by amplification of rRNA. J Clin Microbiol. 1990 Aug;28(8):1751–1759. doi: 10.1128/jcm.28.8.1751-1759.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Crawford J. T. Development of rapid techniques for identification of M. avium infections. Res Microbiol. 1994 Mar-Apr;145(3):177–181. doi: 10.1016/0923-2508(94)90015-9. [DOI] [PubMed] [Google Scholar]
- Denner J. C., Tsang A. Y., Chatterjee D., Brennan P. J. Comprehensive approach to identification of serovars of Mycobacterium avium complex. J Clin Microbiol. 1992 Feb;30(2):473–478. doi: 10.1128/jcm.30.2.473-478.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Falkinham J. O., 3rd Arylsulfatase activity of Mycobacterium avium, M. intracellulare, and M. scrofulaceum. Int J Syst Bacteriol. 1990 Jan;40(1):66–70. doi: 10.1099/00207713-40-1-66. [DOI] [PubMed] [Google Scholar]
- Falkinham J. O., 3rd Epidemiology of Mycobacterium avium infections in the pre- and post-HIV era. Res Microbiol. 1994 Mar-Apr;145(3):169–172. doi: 10.1016/0923-2508(94)90013-2. [DOI] [PubMed] [Google Scholar]
- Frothingham R., Wilson K. H. Molecular phylogeny of the Mycobacterium avium complex demonstrates clinically meaningful divisions. J Infect Dis. 1994 Feb;169(2):305–312. doi: 10.1093/infdis/169.2.305. [DOI] [PubMed] [Google Scholar]
- Guerrero C., Bernasconi C., Burki D., Bodmer T., Telenti A. A novel insertion element from Mycobacterium avium, IS1245, is a specific target for analysis of strain relatedness. J Clin Microbiol. 1995 Feb;33(2):304–307. doi: 10.1128/jcm.33.2.304-307.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hance A. J., Grandchamp B., Lévy-Frébault V., Lecossier D., Rauzier J., Bocart D., Gicquel B. Detection and identification of mycobacteria by amplification of mycobacterial DNA. Mol Microbiol. 1989 Jul;3(7):843–849. doi: 10.1111/j.1365-2958.1989.tb00233.x. [DOI] [PubMed] [Google Scholar]
- Inderlied C. B., Kemper C. A., Bermudez L. E. The Mycobacterium avium complex. Clin Microbiol Rev. 1993 Jul;6(3):266–310. doi: 10.1128/cmr.6.3.266. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lebrun L., Espinasse F., Poveda J. D., Vincent-Levy-Frebault V. Evaluation of nonradioactive DNA probes for identification of mycobacteria. J Clin Microbiol. 1992 Sep;30(9):2476–2478. doi: 10.1128/jcm.30.9.2476-2478.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McFadden J. J., Kunze Z. M., Portaels F., Labrousse V., Rastogi N. Epidemiological and genetic markers, virulence factors and intracellular growth of Mycobacterium avium in AIDS. Res Microbiol. 1992 May;143(4):423-30, discussion 430-6. doi: 10.1016/0923-2508(92)90057-u. [DOI] [PubMed] [Google Scholar]
- Rastogi N., Barrow W. W. Laboratory and clinical aspects of the Mycobacterium avium epidemic: contributing factors associated with variability of drug susceptibility and immune responsiveness, and the multifaceted nature of pathogenicity. Res Microbiol. 1994 Mar-Apr;145(3):167–168. doi: 10.1016/0923-2508(94)90012-4. [DOI] [PubMed] [Google Scholar]
- Saito H., Tomioka H., Sato K., Tasaka H., Dawson D. J. Identification of various serovar strains of Mycobacterium avium complex by using DNA probes specific for Mycobacterium avium and Mycobacterium intracellulare. J Clin Microbiol. 1990 Aug;28(8):1694–1697. doi: 10.1128/jcm.28.8.1694-1697.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sato K., Tomioka H., Saito H. Differential susceptibilities of Mycobacterium avium and Mycobacterium intracellulare to sodium nitrite. J Clin Microbiol. 1992 Nov;30(11):2994–2995. doi: 10.1128/jcm.30.11.2994-2995.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Soini H., Eerola E., Viljanen M. K. Genetic diversity among Mycobacterium avium complex AccuProbe-positive isolates. J Clin Microbiol. 1996 Jan;34(1):55–57. doi: 10.1128/jcm.34.1.55-57.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sritharan V., Iralu J. V., Barker R. H., Jr Specificity of diagnostic PCR amplification for M. avium using the probe pMAV22. Mol Cell Probes. 1995 Feb;9(1):71–74. doi: 10.1016/s0890-8508(95)91067-0. [DOI] [PubMed] [Google Scholar]
- Telenti A., Marchesi F., Balz M., Bally F., Böttger E. C., Bodmer T. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol. 1993 Feb;31(2):175–178. doi: 10.1128/jcm.31.2.175-178.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thierry D., Baugé S., Poveda J. D., Vincent V., Guesdon J. L. Rapid identification of Mycobacterium avium-intracellulare complex strains: clinical practice evaluation of DT6 and DT1 probes. J Infect Dis. 1993 Nov;168(5):1337–1338. doi: 10.1093/infdis/168.5.1337. [DOI] [PubMed] [Google Scholar]
- Thierry D., Matsiota-Bernard P., Nauciel C., Guesdon J. L. Comparison of polymerase chain reaction and non-radioactive hybridization techniques for the identification of Mycobacterium avium strains. Mol Cell Probes. 1994 Dec;8(6):469–471. doi: 10.1006/mcpr.1994.1067. [DOI] [PubMed] [Google Scholar]
- Thierry D., Vincent V., Clément F., Guesdon J. L. Isolation of specific DNA fragments of Mycobacterium avium and their possible use in diagnosis. J Clin Microbiol. 1993 May;31(5):1048–1054. doi: 10.1128/jcm.31.5.1048-1054.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tomioka H., Saito H., Sato K., Dawson D. J. Arylsulfatase activity for differentiating Mycobacterium avium and Mycobacterium intracellulare. J Clin Microbiol. 1990 Sep;28(9):2104–2106. doi: 10.1128/jcm.28.9.2104-2106.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tomioka H., Saito H., Sato K., Tasaka H., Dawson D. J. Identification of Mycobacterium avium complex strains belonging to serovars 21-28 by three commercial DNA probe tests. Tuber Lung Dis. 1993 Apr;74(2):91–95. doi: 10.1016/0962-8479(93)90033-T. [DOI] [PubMed] [Google Scholar]
- Viljanen M. K., Olkkonen L., Katila M. L. Conventional identification characteristics, mycolate and fatty acid composition, and clinical significance of MAIX AccuProbe-positive isolates of Mycobacterium avium complex. J Clin Microbiol. 1993 May;31(5):1376–1378. doi: 10.1128/jcm.31.5.1376-1378.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wayne L. G., Good R. C., Tsang A., Butler R., Dawson D., Groothuis D., Gross W., Hawkins J., Kilburn J., Kubin M. Serovar determination and molecular taxonomic correlation in Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum: a cooperative study of the International Working Group on Mycobacterial Taxonomy. Int J Syst Bacteriol. 1993 Jul;43(3):482–489. doi: 10.1099/00207713-43-3-482. [DOI] [PubMed] [Google Scholar]
- de Lamballerie X., Zandotti C., Vignoli C., Bollet C., de Micco P. A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification. Res Microbiol. 1992 Oct;143(8):785–790. doi: 10.1016/0923-2508(92)90107-y. [DOI] [PubMed] [Google Scholar]