Abstract
Hepatitis C virus genotypes were determined for 358 viremic individuals in Montreal, Canada, by restriction endonuclease analysis of PCR products and phylogenetic analysis of core gene sequences. Types 1, 2, and 3 occurred in 62.8, 14.2, and 13.7%, respectively; types 4 and 5 were found in 3.9 and 4.5%, respectively; and genotypes 6a and 7c and a novel genotype each occurred in 0.3%. Types 4, 6, and 7 and the novel genotype were mostly from persons who had immigrated to Canada.
Full Text
The Full Text of this article is available as a PDF (181.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Andonov A., Chaudhary R. K. Genotyping of Canadian hepatitis C virus isolates by PCR. J Clin Microbiol. 1994 Aug;32(8):2031–2034. doi: 10.1128/jcm.32.8.2031-2034.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bukh J., Miller R. H., Purcell R. H. Genetic heterogeneity of hepatitis C virus: quasispecies and genotypes. Semin Liver Dis. 1995 Feb;15(1):41–63. doi: 10.1055/s-2007-1007262. [DOI] [PubMed] [Google Scholar]
- Collins M. L., Zayati C., Detmer J. J., Daly B., Kolberg J. A., Cha T. A., Irvine B. D., Tucker J., Urdea M. S. Preparation and characterization of RNA standards for use in quantitative branched DNA hybridization assays. Anal Biochem. 1995 Mar 20;226(1):120–129. doi: 10.1006/abio.1995.1199. [DOI] [PubMed] [Google Scholar]
- Davidson F., Simmonds P., Ferguson J. C., Jarvis L. M., Dow B. C., Follett E. A., Seed C. R., Krusius T., Lin C., Medgyesi G. A. Survey of major genotypes and subtypes of hepatitis C virus using RFLP of sequences amplified from the 5' non-coding region. J Gen Virol. 1995 May;76(Pt 5):1197–1204. doi: 10.1099/0022-1317-76-5-1197. [DOI] [PubMed] [Google Scholar]
- Lau J. Y., Davis G. L., Prescott L. E., Maertens G., Lindsay K. L., Qian K., Mizokami M., Simmonds P. Distribution of hepatitis C virus genotypes determined by line probe assay in patients with chronic hepatitis C seen at tertiary referral centers in the United States. Hepatitis Interventional Therapy Group. Ann Intern Med. 1996 May 15;124(10):868–876. doi: 10.7326/0003-4819-124-10-199605150-00002. [DOI] [PubMed] [Google Scholar]
- Mellor J., Holmes E. C., Jarvis L. M., Yap P. L., Simmonds P. Investigation of the pattern of hepatitis C virus sequence diversity in different geographical regions: implications for virus classification. The International HCV Collaborative Study Group. J Gen Virol. 1995 Oct;76(Pt 10):2493–2507. doi: 10.1099/0022-1317-76-10-2493. [DOI] [PubMed] [Google Scholar]
- Murphy D. G., Willems B., Vincelette J., Bernier L., Côté J., Delage G. Biological and clinicopathological features associated with hepatitis C virus type 5 infections. J Hepatol. 1996 Jan;24(1):109–113. doi: 10.1016/s0168-8278(96)80194-2. [DOI] [PubMed] [Google Scholar]
- Murphy D., Willems B., Delage G. Use of the 5' noncoding region for genotyping hepatitis C virus. J Infect Dis. 1994 Feb;169(2):473–475. doi: 10.1093/infdis/169.2.473. [DOI] [PubMed] [Google Scholar]
- Pawlotsky J. M., Tsakiris L., Roudot-Thoraval F., Pellet C., Stuyver L., Duval J., Dhumeaux D. Relationship between hepatitis C virus genotypes and sources of infection in patients with chronic hepatitis C. J Infect Dis. 1995 Jun;171(6):1607–1610. doi: 10.1093/infdis/171.6.1607. [DOI] [PubMed] [Google Scholar]
- Simmonds P., Alberti A., Alter H. J., Bonino F., Bradley D. W., Brechot C., Brouwer J. T., Chan S. W., Chayama K., Chen D. S. A proposed system for the nomenclature of hepatitis C viral genotypes. Hepatology. 1994 May;19(5):1321–1324. [PubMed] [Google Scholar]
- Smith D. B., Mellor J., Jarvis L. M., Davidson F., Kolberg J., Urdea M., Yap P. L., Simmonds P. Variation of the hepatitis C virus 5' non-coding region: implications for secondary structure, virus detection and typing. The International HCV Collaborative Study Group. J Gen Virol. 1995 Jul;76(Pt 7):1749–1761. doi: 10.1099/0022-1317-76-7-1749. [DOI] [PubMed] [Google Scholar]
- Stuyver L., Wyseur A., van Arnhem W., Lunel F., Laurent-Puig P., Pawlotsky J. M., Kleter B., Bassit L., Nkengasong J., van Doorn L. J. Hepatitis C virus genotyping by means of 5'-UR/core line probe assays and molecular analysis of untypeable samples. Virus Res. 1995 Oct;38(2-3):137–157. doi: 10.1016/0168-1702(95)00052-r. [DOI] [PubMed] [Google Scholar]
- Tokita H., Okamoto H., Iizuka H., Kishimoto J., Tsuda F., Lesmana L. A., Miyakawa Y., Mayumi M. Hepatitis C virus variants from Jakarta, Indonesia classifiable into novel genotypes in the second (2e and 2f), tenth (10a) and eleventh (11a) genetic groups. J Gen Virol. 1996 Feb;77(Pt 2):293–301. doi: 10.1099/0022-1317-77-2-293. [DOI] [PubMed] [Google Scholar]