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. 1996 Nov;34(11):2819–2821. doi: 10.1128/jcm.34.11.2819-2821.1996

Use of an alkaline phosphatase-conjugated oligonucleotide probe for the gene encoding the bundle-forming pilus of enteropathogenic Escherichia coli.

K Nagayama 1, Z Bi 1, T Oguchi 1, Y Takarada 1, S Shibata 1, T Honda 1
PMCID: PMC229410  PMID: 8897189

Abstract

An alkaline phosphatase-conjugated 29-base oligonucleotide probe was developed to detect the gene encoding the bundle-forming pilus of enteropathogenic Escherichia coli. The sensitivity and specificity of the probe versus the results of localized adherence in the HEp-2 cell assay and fluorescent actin staining assay positivity were 95.7 and 100%, respectively.

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Selected References

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