Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1996 Nov;34(11):2843–2846. doi: 10.1128/jcm.34.11.2843-2846.1996

Specificity of IS6110-based amplification assays for Mycobacterium tuberculosis complex.

T J Hellyer 1, L E DesJardin 1, M K Assaf 1, J H Bates 1, M D Cave 1, K D Eisenach 1
PMCID: PMC229418  PMID: 8897197

Abstract

The specificity of IS6110 for the Mycobacterium tuberculosis complex has recently been questioned. We observed no cross-reaction with 27 nontuberculous mycobacteria using strand displacement- and PCR-based amplification of the nucleotide 970 to 1026 and 762 to 865 regions of IS6110. These data support use of selected regions of IS6110 as M. tuberculosis complex-specific targets.

Full Text

The Full Text of this article is available as a PDF (670.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersen A. B., Thybo S., Godfrey-Faussett P., Stoker N. G. Polymerase chain reaction for detection of Mycobacterium tuberculosis in sputum. Eur J Clin Microbiol Infect Dis. 1993 Dec;12(12):922–927. doi: 10.1007/BF01992166. [DOI] [PubMed] [Google Scholar]
  2. Clarridge J. E., 3rd, Shawar R. M., Shinnick T. M., Plikaytis B. B. Large-scale use of polymerase chain reaction for detection of Mycobacterium tuberculosis in a routine mycobacteriology laboratory. J Clin Microbiol. 1993 Aug;31(8):2049–2056. doi: 10.1128/jcm.31.8.2049-2056.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Desjardin L. E., Perkins M. D., Teixeira L., Cave M. D., Eisenach K. D. Alkaline decontamination of sputum specimens adversely affects stability of mycobacterial mRNA. J Clin Microbiol. 1996 Oct;34(10):2435–2439. doi: 10.1128/jcm.34.10.2435-2439.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Down J. A., O'Connell M. A., Dey M. S., Walters A. H., Howard D. R., Little M. C., Keating W. E., Zwadyk P., Jr, Haaland P. D., McLaurin D. A., 3rd Detection of Mycobacterium tuberculosis in respiratory specimens by strand displacement amplification of DNA. J Clin Microbiol. 1996 Apr;34(4):860–865. doi: 10.1128/jcm.34.4.860-865.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eisenach K. D., Cave M. D., Bates J. H., Crawford J. T. Polymerase chain reaction amplification of a repetitive DNA sequence specific for Mycobacterium tuberculosis. J Infect Dis. 1990 May;161(5):977–981. doi: 10.1093/infdis/161.5.977. [DOI] [PubMed] [Google Scholar]
  6. Eisenach K. D., Sifford M. D., Cave M. D., Bates J. H., Crawford J. T. Detection of Mycobacterium tuberculosis in sputum samples using a polymerase chain reaction. Am Rev Respir Dis. 1991 Nov;144(5):1160–1163. doi: 10.1164/ajrccm/144.5.1160. [DOI] [PubMed] [Google Scholar]
  7. Hellyer T. J., Fletcher T. W., Bates J. H., Stead W. W., Templeton G. L., Cave M. D., Eisenach K. D. Strand displacement amplification and the polymerase chain reaction for monitoring response to treatment in patients with pulmonary tuberculosis. J Infect Dis. 1996 Apr;173(4):934–941. doi: 10.1093/infdis/173.4.934. [DOI] [PubMed] [Google Scholar]
  8. Iovannisci D. M., Winn-Deen E. S. Ligation amplification and fluorescence detection of Mycobacterium tuberculosis DNA. Mol Cell Probes. 1993 Feb;7(1):35–43. doi: 10.1006/mcpr.1993.1005. [DOI] [PubMed] [Google Scholar]
  9. Kent L., McHugh T. D., Billington O., Dale J. W., Gillespie S. H. Demonstration of homology between IS6110 of Mycobacterium tuberculosis and DNAs of other Mycobacterium spp.? J Clin Microbiol. 1995 Sep;33(9):2290–2293. doi: 10.1128/jcm.33.9.2290-2293.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kox L. F., Rhienthong D., Miranda A. M., Udomsantisuk N., Ellis K., van Leeuwen J., van Heusden S., Kuijper S., Kolk A. H. A more reliable PCR for detection of Mycobacterium tuberculosis in clinical samples. J Clin Microbiol. 1994 Mar;32(3):672–678. doi: 10.1128/jcm.32.3.672-678.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Longo M. C., Berninger M. S., Hartley J. L. Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions. Gene. 1990 Sep 1;93(1):125–128. doi: 10.1016/0378-1119(90)90145-h. [DOI] [PubMed] [Google Scholar]
  12. Nolte F. S., Metchock B., McGowan J. E., Jr, Edwards A., Okwumabua O., Thurmond C., Mitchell P. S., Plikaytis B., Shinnick T. Direct detection of Mycobacterium tuberculosis in sputum by polymerase chain reaction and DNA hybridization. J Clin Microbiol. 1993 Jul;31(7):1777–1782. doi: 10.1128/jcm.31.7.1777-1782.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Noordhoek G. T., Kolk A. H., Bjune G., Catty D., Dale J. W., Fine P. E., Godfrey-Faussett P., Cho S. N., Shinnick T., Svenson S. B. Sensitivity and specificity of PCR for detection of Mycobacterium tuberculosis: a blind comparison study among seven laboratories. J Clin Microbiol. 1994 Feb;32(2):277–284. doi: 10.1128/jcm.32.2.277-284.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pietrzak J., Frei R., Senn H. P., Moroni C. Comparison of polymerase chain reaction with standard methods in the diagnosis of Mycobacterium tuberculosis infection. Eur J Clin Microbiol Infect Dis. 1994 Dec;13(12):1079–1083. doi: 10.1007/BF02111834. [DOI] [PubMed] [Google Scholar]
  15. Querol J. M., Farga M. A., Granda D., Gimeno C., García-de-Lomas J. The utility of polymerase chain reaction (PCR) in the diagnosis of pulmonary tuberculosis. Chest. 1995 Jun;107(6):1631–1635. doi: 10.1378/chest.107.6.1631. [DOI] [PubMed] [Google Scholar]
  16. Rish J. A., Eisenach K. D., Cave M. D., Reddy M. V., Gangadharam P. R., Bates J. H. Polymerase chain reaction detection of Mycobacterium tuberculosis in formalin-fixed tissue. Am J Respir Crit Care Med. 1996 Apr;153(4 Pt 1):1419–1423. doi: 10.1164/ajrccm.153.4.8616575. [DOI] [PubMed] [Google Scholar]
  17. Savić B., Sjöbring U., Alugupalli S., Larsson L., Miörner H. Evaluation of polymerase chain reaction, tuberculostearic acid analysis, and direct microscopy for the detection of Mycobacterium tuberculosis in sputum. J Infect Dis. 1992 Nov;166(5):1177–1180. doi: 10.1093/infdis/166.5.1177. [DOI] [PubMed] [Google Scholar]
  18. Schluger N. W., Kinney D., Harkin T. J., Rom W. N. Clinical utility of the polymerase chain reaction in the diagnosis of infections due to Mycobacterium tuberculosis. Chest. 1994 Apr;105(4):1116–1121. doi: 10.1378/chest.105.4.1116. [DOI] [PubMed] [Google Scholar]
  19. Shawar R. M., el-Zaatari F. A., Nataraj A., Clarridge J. E. Detection of Mycobacterium tuberculosis in clinical samples by two-step polymerase chain reaction and nonisotopic hybridization methods. J Clin Microbiol. 1993 Jan;31(1):61–65. doi: 10.1128/jcm.31.1.61-65.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Spargo C. A., Haaland P. D., Jurgensen S. R., Shank D. D., Walker G. T. Chemiluminescent detection of strand displacement amplified DNA from species comprising the Mycobacterium tuberculosis complex. Mol Cell Probes. 1993 Oct;7(5):395–404. doi: 10.1006/mcpr.1993.1058. [DOI] [PubMed] [Google Scholar]
  21. Thierry D., Brisson-Noël A., Vincent-Lévy-Frébault V., Nguyen S., Guesdon J. L., Gicquel B. Characterization of a Mycobacterium tuberculosis insertion sequence, IS6110, and its application in diagnosis. J Clin Microbiol. 1990 Dec;28(12):2668–2673. doi: 10.1128/jcm.28.12.2668-2673.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Thierry D., Cave M. D., Eisenach K. D., Crawford J. T., Bates J. H., Gicquel B., Guesdon J. L. IS6110, an IS-like element of Mycobacterium tuberculosis complex. Nucleic Acids Res. 1990 Jan 11;18(1):188–188. doi: 10.1093/nar/18.1.188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Walker G. T., Fraiser M. S., Schram J. L., Little M. C., Nadeau J. G., Malinowski D. P. Strand displacement amplification--an isothermal, in vitro DNA amplification technique. Nucleic Acids Res. 1992 Apr 11;20(7):1691–1696. doi: 10.1093/nar/20.7.1691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Walker G. T., Little M. C., Nadeau J. G., Shank D. D. Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system. Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):392–396. doi: 10.1073/pnas.89.1.392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wilson S. M., McNerney R., Nye P. M., Godfrey-Faussett P. D., Stoker N. G., Voller A. Progress toward a simplified polymerase chain reaction and its application to diagnosis of tuberculosis. J Clin Microbiol. 1993 Apr;31(4):776–782. doi: 10.1128/jcm.31.4.776-782.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES