Abstract
DNA encoding two repeat units of 120-kDa protein of Ehrlichia chaffeensis was cloned into the expression vector pGEX and expressed in Escherichia coli. The sensitivity and specificity of a dot blot assay for detection of human antibodies with the recombinant protein were 86 and 100%, respectively, compared with an indirect immunofluorescence assay.
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- Anderson B. E., Dawson J. E., Jones D. C., Wilson K. H. Ehrlichia chaffeensis, a new species associated with human ehrlichiosis. J Clin Microbiol. 1991 Dec;29(12):2838–2842. doi: 10.1128/jcm.29.12.2838-2842.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chen S. M., Dumler J. S., Feng H. M., Walker D. H. Identification of the antigenic constituents of Ehrlichia chaffeensis. Am J Trop Med Hyg. 1994 Jan;50(1):52–58. [PubMed] [Google Scholar]
- Dawson J. E., Anderson B. E., Fishbein D. B., Sanchez J. L., Goldsmith C. S., Wilson K. H., Duntley C. W. Isolation and characterization of an Ehrlichia sp. from a patient diagnosed with human ehrlichiosis. J Clin Microbiol. 1991 Dec;29(12):2741–2745. doi: 10.1128/jcm.29.12.2741-2745.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dawson J. E., Fishbein D. B., Eng T. R., Redus M. A., Green N. R. Diagnosis of human ehrlichiosis with the indirect fluorescent antibody test: kinetics and specificity. J Infect Dis. 1990 Jul;162(1):91–95. doi: 10.1093/infdis/162.1.91. [DOI] [PubMed] [Google Scholar]
- Dawson J. E., Rikihisa Y., Ewing S. A., Fishbein D. B. Serologic diagnosis of human ehrlichiosis using two Ehrlichia canis isolates. J Infect Dis. 1991 Mar;163(3):564–567. doi: 10.1093/infdis/163.3.564. [DOI] [PubMed] [Google Scholar]
- Dawson J. E., Warner C. K., Standaert S., Olson J. G. The interface between research and the diagnosis of an emerging tick-borne disease, human ehrlichiosis due to Ehrlichia chaffeensis. Arch Intern Med. 1996 Jan 22;156(2):137–142. [PubMed] [Google Scholar]
- Fishbein D. B., Dawson J. E., Robinson L. E. Human ehrlichiosis in the United States, 1985 to 1990. Ann Intern Med. 1994 May 1;120(9):736–743. doi: 10.7326/0003-4819-120-9-199405010-00003. [DOI] [PubMed] [Google Scholar]
- Fishbein D. B., Sawyer L. A., Holland C. J., Hayes E. B., Okoroanyanwu W., Williams D., Sikes K., Ristic M., McDade J. E. Unexplained febrile illnesses after exposure to ticks. Infection with an Ehrlichia? JAMA. 1987 Jun 12;257(22):3100–3104. [PubMed] [Google Scholar]
- Maeda K., Markowitz N., Hawley R. C., Ristic M., Cox D., McDade J. E. Human infection with Ehrlichia canis, a leukocytic rickettsia. N Engl J Med. 1987 Apr 2;316(14):853–856. doi: 10.1056/NEJM198704023161406. [DOI] [PubMed] [Google Scholar]