Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1996 Dec;34(12):2942–2946. doi: 10.1128/jcm.34.12.2942-2946.1996

Characterization of multiple-antibiotic-resistant Salmonella typhimurium stains: molecular epidemiology of PER-1-producing isolates and evidence for nosocomial plasmid exchange by a clone.

H Vahaboglu 1, S Dodanli 1, C Eroglu 1, R Oztürk 1, G Soyletir 1, I Yildirim 1, V Avkan 1
PMCID: PMC229438  PMID: 8940427

Abstract

We characterized epidemiologic and genetic features of nosocomially originated multiple-antibiotic-resistant Salmonella typhimurium isolates from two hospitals. A total of 32 multiply resistant strains, isolated during a 28-month period, were studied. Four resistance phenotypes were distinguished on the basis of the results of disc diffusion tests. Group 1 was resistant to chloramphenicol, gentamicin, tobramycin, amikacin, and the newer cephalosporins because of the production of an extended-spectrum beta-lactamase (PER-1). Group 2 exhibited the same pattern plus resistance to sulfamethoxazole-trimethoprim (Sxt). Except for Sxt resistance, dominant phenotypes of both groups were transferred on an identical plasmid, pSTI1 (81 MDa). Group 3 was resistant to ampicillin, chloramphenicol, gentamicin, tobramycin, and Sxt. This pattern was also transferred on an 81-MDa plasmid (pSTI2) which differed from pSTI1 on the basis of EcoRI and HindIII restriction fragments. Group 4 was resistant to ampicillin, chloramphenicol, and tetracycline, and a 74-MDa nonconjugative plasmid was detected. Restriction fragment length polymorphism of RNA-encoding DNA and arbitrarily primed PCR tests revealed that bacteria from groups 1, 2, and 3 were clonally related. Epidemiologic data also supported the clonal-dissemination hypothesis. We concluded that S. typhimurium isolates acquire and exchange multiple-resistance plasmids in hospital microflora.

Full Text

The Full Text of this article is available as a PDF (299.7 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Archambaud M., Gerbaud G., Labau E., Marty N., Courvalin P. Possible in-vivo transfer of beta-lactamase TEM-3 from Klebsiella pneumoniae to Salmonella kedougou. J Antimicrob Chemother. 1991 Apr;27(4):427–436. doi: 10.1093/jac/27.4.427. [DOI] [PubMed] [Google Scholar]
  2. Boom R., Sol C. J., Salimans M. M., Jansen C. L., Wertheim-van Dillen P. M., van der Noordaa J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol. 1990 Mar;28(3):495–503. doi: 10.1128/jcm.28.3.495-503.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Collis C. M., Hall R. M. Expression of antibiotic resistance genes in the integrated cassettes of integrons. Antimicrob Agents Chemother. 1995 Jan;39(1):155–162. doi: 10.1128/aac.39.1.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Esteban E., Snipes K., Hird D., Kasten R., Kinde H. Use of ribotyping for characterization of Salmonella serotypes. J Clin Microbiol. 1993 Feb;31(2):233–237. doi: 10.1128/jcm.31.2.233-237.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hammami A., Arlet G., Ben Redjeb S., Grimont F., Ben Hassen A., Rekik A., Philippon A. Nosocomial outbreak of acute gastroenteritis in a neonatal intensive care unit in Tunisia caused by multiply drug resistant Salmonella wien producing SHV-2 beta-lactamase. Eur J Clin Microbiol Infect Dis. 1991 Aug;10(8):641–646. doi: 10.1007/BF01975816. [DOI] [PubMed] [Google Scholar]
  6. Jacoby G. A., Sutton L. Properties of plasmids responsible for production of extended-spectrum beta-lactamases. Antimicrob Agents Chemother. 1991 Jan;35(1):164–169. doi: 10.1128/aac.35.1.164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mathew A., Harris A. M., Marshall M. J., Ross G. W. The use of analytical isoelectric focusing for detection and identification of beta-lactamases. J Gen Microbiol. 1975 May;88(1):169–178. doi: 10.1099/00221287-88-1-169. [DOI] [PubMed] [Google Scholar]
  9. Murray B. E. Resistance of Shigella, Salmonella, and other selected enteric pathogens to antimicrobial agents. Rev Infect Dis. 1986 May-Jun;8 (Suppl 2):S172–S181. doi: 10.1093/clinids/8.supplement_2.s172. [DOI] [PubMed] [Google Scholar]
  10. Nordmann P., Naas T. Sequence analysis of PER-1 extended-spectrum beta-lactamase from Pseudomonas aeruginosa and comparison with class A beta-lactamases. Antimicrob Agents Chemother. 1994 Jan;38(1):104–114. doi: 10.1128/aac.38.1.104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Poupart M. C., Chanal C., Sirot D., Labia R., Sirot J. Identification of CTX-2, a novel cefotaximase from a Salmonella mbandaka isolate. Antimicrob Agents Chemother. 1991 Jul;35(7):1498–1500. doi: 10.1128/aac.35.7.1498. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  13. Spika J. S., Waterman S. H., Hoo G. W., St Louis M. E., Pacer R. E., James S. M., Bissett M. L., Mayer L. W., Chiu J. Y., Hall B. Chloramphenicol-resistant Salmonella newport traced through hamburger to dairy farms. A major persisting source of human salmonellosis in California. N Engl J Med. 1987 Mar 5;316(10):565–570. doi: 10.1056/NEJM198703053161001. [DOI] [PubMed] [Google Scholar]
  14. Taylor D. N., Wachsmuth I. K., Shangkuan Y. H., Schmidt E. V., Barrett T. J., Schrader J. S., Scherach C. S., McGee H. B., Feldman R. A., Brenner D. J. Salmonellosis associated with marijuana: a multistate outbreak traced by plasmid fingerprinting. N Engl J Med. 1982 May 27;306(21):1249–1253. doi: 10.1056/NEJM198205273062101. [DOI] [PubMed] [Google Scholar]
  15. Usera M. A., Popovic T., Bopp C. A., Strockbine N. A. Molecular subtyping of Salmonella enteritidis phage type 8 strains from the United States. J Clin Microbiol. 1994 Jan;32(1):194–198. doi: 10.1128/jcm.32.1.194-198.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Vahaboglu H., Hall L. M., Mulazimoglu L., Dodanli S., Yildirim I., Livermore D. M. Resistance to extended-spectrum cephalosporins, caused by PER-1 beta-lactamase, in Salmonella typhimurium from Istanbul, Turkey. J Med Microbiol. 1995 Oct;43(4):294–299. doi: 10.1099/00222615-43-4-294. [DOI] [PubMed] [Google Scholar]
  17. Versalovic J., Koeuth T., Lupski J. R. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res. 1991 Dec 25;19(24):6823–6831. doi: 10.1093/nar/19.24.6823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. de la Maza L., Miller S. I., Ferraro M. J. Use of commercially available rapid chloramphenicol acetyltransferase test to detect resistance in Salmonella species. J Clin Microbiol. 1990 Aug;28(8):1867–1869. doi: 10.1128/jcm.28.8.1867-1869.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. van Belkum A. DNA fingerprinting of medically important microorganisms by use of PCR. Clin Microbiol Rev. 1994 Apr;7(2):174–184. doi: 10.1128/cmr.7.2.174. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES