Abstract
Polyphasic taxonomic methods were employed to characterize a new species of slowly growing, nonpigmented mycobacteria. We propose the name Mycobacterium triplex sp. nov. for this new taxon. Conventional identification testing demonstrated a group of similar organisms that were geographically widespread in the United States. Commercially available nucleic-acid probes specific for the Mycobacterium avium complex were unreactive for these strains. High-performance liquid chromatography analysis of the mycolic acids revealed mycolate profiles that closely resembled Mycobacterium simiae. Comparative 16S rRNA sequence data confirmed the phylogenetic relationship of the strains with the slowly growing mycobacteria. Representative-type strains have been deposited in the American Type Culture Collection as strain ATCC 700071 [corrected].
Full Text
The Full Text of this article is available as a PDF (195.4 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Butler W. R., Jost K. C., Jr, Kilburn J. O. Identification of mycobacteria by high-performance liquid chromatography. J Clin Microbiol. 1991 Nov;29(11):2468–2472. doi: 10.1128/jcm.29.11.2468-2472.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Butler W. R., O'Connor S. P., Yakrus M. A., Smithwick R. W., Plikaytis B. B., Moss C. W., Floyd M. M., Woodley C. L., Kilburn J. O., Vadney F. S. Mycobacterium celatum sp. nov. Int J Syst Bacteriol. 1993 Jul;43(3):539–548. doi: 10.1099/00207713-43-3-539. [DOI] [PubMed] [Google Scholar]
- Butler W. R., Thibert L., Kilburn J. O. Identification of Mycobacterium avium complex strains and some similar species by high-performance liquid chromatography. J Clin Microbiol. 1992 Oct;30(10):2698–2704. doi: 10.1128/jcm.30.10.2698-2704.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Böddinghaus B., Rogall T., Flohr T., Blöcker H., Böttger E. C. Detection and identification of mycobacteria by amplification of rRNA. J Clin Microbiol. 1990 Aug;28(8):1751–1759. doi: 10.1128/jcm.28.8.1751-1759.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Böttger E. C. Rapid determination of bacterial ribosomal RNA sequences by direct sequencing of enzymatically amplified DNA. FEMS Microbiol Lett. 1989 Nov;53(1-2):171–176. doi: 10.1016/0378-1097(89)90386-8. [DOI] [PubMed] [Google Scholar]
- Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edwards U., Rogall T., Blöcker H., Emde M., Böttger E. C. Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res. 1989 Oct 11;17(19):7843–7853. doi: 10.1093/nar/17.19.7843. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Glickman S. E., Kilburn J. O., Butler W. R., Ramos L. S. Rapid identification of mycolic acid patterns of mycobacteria by high-performance liquid chromatography using pattern recognition software and a Mycobacterium library. J Clin Microbiol. 1994 Mar;32(3):740–745. doi: 10.1128/jcm.32.3.740-745.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kazda J., Stackebrandt E., Smida J., Minnikin D. E., Daffe M., Parlett J. H., Pitulle C. Mycobacterium cookii sp. nov. Int J Syst Bacteriol. 1990 Jul;40(3):217–223. doi: 10.1099/00207713-40-3-217. [DOI] [PubMed] [Google Scholar]
- Kirschner P., Springer B., Vogel U., Meier A., Wrede A., Kiekenbeck M., Bange F. C., Böttger E. C. Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. J Clin Microbiol. 1993 Nov;31(11):2882–2889. doi: 10.1128/jcm.31.11.2882-2889.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meier A., Kirschner P., Schröder K. H., Wolters J., Kroppenstedt R. M., Böttger E. C. Mycobacterium intermedium sp. nov. Int J Syst Bacteriol. 1993 Apr;43(2):204–209. doi: 10.1099/00207713-43-2-204. [DOI] [PubMed] [Google Scholar]
- Pitulle C., Dorsch M., Kazda J., Wolters J., Stackebrandt E. Phylogeny of rapidly growing members of the genus Mycobacterium. Int J Syst Bacteriol. 1992 Jul;42(3):337–343. doi: 10.1099/00207713-42-3-337. [DOI] [PubMed] [Google Scholar]
- Plikaytis B. B., Gelber R. H., Shinnick T. M. Rapid and sensitive detection of Mycobacterium leprae using a nested-primer gene amplification assay. J Clin Microbiol. 1990 Sep;28(9):1913–1917. doi: 10.1128/jcm.28.9.1913-1917.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rogall T., Flohr T., Böttger E. C. Differentiation of Mycobacterium species by direct sequencing of amplified DNA. J Gen Microbiol. 1990 Sep;136(9):1915–1920. doi: 10.1099/00221287-136-9-1915. [DOI] [PubMed] [Google Scholar]
- Rogall T., Wolters J., Flohr T., Böttger E. C. Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium. Int J Syst Bacteriol. 1990 Oct;40(4):323–330. doi: 10.1099/00207713-40-4-323. [DOI] [PubMed] [Google Scholar]
- Saitou N., Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987 Jul;4(4):406–425. doi: 10.1093/oxfordjournals.molbev.a040454. [DOI] [PubMed] [Google Scholar]
- Springer B., Böttger E. C., Kirschner P., Wallace R. J., Jr Phylogeny of the Mycobacterium chelonae-like organism based on partial sequencing of the 16S rRNA gene and proposal of Mycobacterium mucogenicum sp. nov. Int J Syst Bacteriol. 1995 Apr;45(2):262–267. doi: 10.1099/00207713-45-2-262. [DOI] [PubMed] [Google Scholar]
- Springer B., Kirschner P., Rost-Meyer G., Schröder K. H., Kroppenstedt R. M., Böttger E. C. Mycobacterium interjectum, a new species isolated from a patient with chronic lymphadenitis. J Clin Microbiol. 1993 Dec;31(12):3083–3089. doi: 10.1128/jcm.31.12.3083-3089.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stahl D. A., Urbance J. W. The division between fast- and slow-growing species corresponds to natural relationships among the mycobacteria. J Bacteriol. 1990 Jan;172(1):116–124. doi: 10.1128/jb.172.1.116-124.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Suzuki Y., Nagata A., Ono Y., Yamada T. Complete nucleotide sequence of the 16S rRNA gene of Mycobacterium bovis BCG. J Bacteriol. 1988 Jun;170(6):2886–2889. doi: 10.1128/jb.170.6.2886-2889.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]