Abstract
Lipovitellin-salt-mannitol (LSM) plate medium was examined for its ability to directly isolate, recover, and presumptively identify Staphylococcus aureus from 418 clinical specimens. The criteria for medium evaluation included colony morphology reactions, selectivity, and ease of isolation. For 298 specimens used for screening, LSM agar medium was compared with the other conventional media used, mannitol salt agar (MSA), 5% horse blood agar (HBA), and phenolphthalein phosphate agar (PPA), to detect and recover S. aureus and methicillin-resistant S. aureus. The results indicated that LSM agar is more effective than MSA, HBA, or PPA for the recovery and isolation of S. aureus and methicillin-resistant S. aureus. On a replicator multipoint inoculation system, we compared the reactions on LSM agar, MSA, and DNase agar of 227 different strains of staphylococci, which included 178 different strains of S. aureus and 49 different strains of coagulase-negative staphylococci isolated from clinical specimens. By using the lipovitellin precipitation activity and mannitol fermentation characteristics, LSM agar gave a 100% correlation in presumptively identifying S. aureus. LSM agar may be an alternative plate medium for large hospital extensive screening for the detection and isolation of S. aureus.
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- BARBER M. Methicillin-resistant staphylococci. J Clin Pathol. 1961 Jul;14:385–393. doi: 10.1136/jcp.14.4.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burman L. G., Ostensson R. Time- and media-saving testing and identification of microorganisms by multipoint inoculation on undivided agar plates. J Clin Microbiol. 1978 Aug;8(2):219–227. doi: 10.1128/jcm.8.2.219-227.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chapman G. H. The Significance of Sodium Chloride in Studies of Staphylococci. J Bacteriol. 1945 Aug;50(2):201–203. [PMC free article] [PubMed] [Google Scholar]
- Churcher G. M. A screening test for the detection of methicillin-resistant staphylococci. J Clin Pathol. 1968 Mar;21(2):213–217. doi: 10.1136/jcp.21.2.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GILLESPIE W. A., ALDER V. G. Production of opacity in egg-yolk media by coagulase-positive staphylococci. J Pathol Bacteriol. 1952 Jan;64(1):187–200. doi: 10.1002/path.1700640119. [DOI] [PubMed] [Google Scholar]
- Gunn B. A., Dunkelberg W. E., Jr, Creitz J. R. Clinical evaluation of 2 -LSM medium for primary isolation and identification of staphylococci. Am J Clin Pathol. 1972 Feb;57(2):236–240. doi: 10.1093/ajcp/57.2.236. [DOI] [PubMed] [Google Scholar]
- La Zonby J. G., Starzyk M. J. Screening method for recovery of methicillin-resistant Staphylococcus aureus from primary plates. J Clin Microbiol. 1986 Aug;24(2):186–188. doi: 10.1128/jcm.24.2.186-188.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lally R. T., Ederer M. N., Woolfrey B. F. Evaluation of mannitol salt agar with oxacillin as a screening medium for methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 1985 Oct;22(4):501–504. doi: 10.1128/jcm.22.4.501-504.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reuther J. W. A simplified system for the identification of staphylococci by multipoint inoculation of test media. J Med Microbiol. 1986 Sep;22(2):179–182. doi: 10.1099/00222615-22-2-179. [DOI] [PubMed] [Google Scholar]
- SHAH D. B., WILSON J. B. EGG YOLK FACTOR OF STAPHYLOCOCCUS AUREUS. I. NATURE OF THE SUBSTRATE AND ENZYME INVOLVED IN THE EGG YOLK OPACITY REACTION. J Bacteriol. 1963 Mar;85:516–521. doi: 10.1128/jb.85.3.516-521.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SHAH D. B., WILSON J. B. EGG YOLK FACTOR OF STAPHYLOCOCCUS AUREUS. II. CHARACTERIZATION OF THE LIPASE ACTIVITY. J Bacteriol. 1965 Apr;89:949–953. doi: 10.1128/jb.89.4.949-953.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thornsberry C., Caruthers J. Q., Baker C. N. Effect of temperature on the in vitro susceptibility of Staphylococcus aureus to penicillinase-resistant penicillins. Antimicrob Agents Chemother. 1973 Sep;4(3):263–269. doi: 10.1128/aac.4.3.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Voss A., Milatovic D., Wallrauch-Schwarz C., Rosdahl V. T., Braveny I. Methicillin-resistant Staphylococcus aureus in Europe. Eur J Clin Microbiol Infect Dis. 1994 Jan;13(1):50–55. doi: 10.1007/BF02026127. [DOI] [PubMed] [Google Scholar]