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. 1996 Dec;34(12):3075–3084. doi: 10.1128/jcm.34.12.3075-3084.1996

Identification of staphylococci with a self-educating system using fatty acid analysis and biochemical tests.

R J Behme 1, R Shuttleworth 1, A McNabb 1, W D Colby 1
PMCID: PMC229462  PMID: 8940451

Abstract

We characterized all of the 35 aerobic taxa of the genus Staphylococcus by using an objective, self-learning system combining both whole-cell fatty acid (FA) analysis and the results of 35 biochemical tests. Isolates were compared with the type strain for each taxon to generate an FA profile library and a biochemical table of test responses. Isolates were accepted into the system if they had a similarity index of > or = 0.6 for a taxon within the FA profile library and if they were identified as the same taxon by a computer program using a probability matrix constructed from the biochemical data. These stringent criteria led to acceptance of 1,117 strains assigned to legitimate taxa. Additional FA groups were assembled from selected strains that did not meet the inclusion criteria based on the type strains and were added to the system as separate entries. Currently, 1,512 isolates have bee accepted into the system. This approach has resulted in a comprehensive table of biochemical test results and a FA profile library, which together provide a practical system for valid identifications.

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Selected References

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  1. Baker J. S. Comparison of various methods for differentiation of staphylococci and micrococci. J Clin Microbiol. 1984 Jun;19(6):875–879. doi: 10.1128/jcm.19.6.875-879.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bannerman T. L., Kloos W. E. Staphylococcus capitis subsp. ureolyticus subsp. nov. from human skin. Int J Syst Bacteriol. 1991 Jan;41(1):144–147. doi: 10.1099/00207713-41-1-144. [DOI] [PubMed] [Google Scholar]
  3. Chesneau O., Morvan A., Grimont F., Labischinski H., el Solh N. Staphylococcus pasteuri sp. nov., isolated from human, animal, and food specimens. Int J Syst Bacteriol. 1993 Apr;43(2):237–244. doi: 10.1099/00207713-43-2-237. [DOI] [PubMed] [Google Scholar]
  4. De Buyser M. L., Morvan A., Aubert S., Dilasser F., el Solh N. Evaluation of a ribosomal RNA gene probe for the identification of species and subspecies within the genus Staphylococcus. J Gen Microbiol. 1992 May;138(5):889–899. doi: 10.1099/00221287-138-5-889. [DOI] [PubMed] [Google Scholar]
  5. De Buyser M. L., Morvan A., Grimont F., el Solh N. Characterization of Staphylococcus species by ribosomal RNA gene restriction patterns. J Gen Microbiol. 1989 Apr;135(4):989–999. doi: 10.1099/00221287-135-4-989. [DOI] [PubMed] [Google Scholar]
  6. Eerola E., Lehtonen O. P. Optimal data processing procedure for automatic bacterial identification by gas-liquid chromatography of cellular fatty acids. J Clin Microbiol. 1988 Sep;26(9):1745–1753. doi: 10.1128/jcm.26.9.1745-1753.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Faller A., Schleifer K. H. Modified oxidase and benzidine tests for separation of staphylococci from micrococci. J Clin Microbiol. 1981 Jun;13(6):1031–1035. doi: 10.1128/jcm.13.6.1031-1035.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. George C. G., Kloos W. E. Comparison of the SmaI-digested chromosomes of Staphylococcus epidermidis and the closely related species Staphylococcus capitis and Staphylococcus caprae. Int J Syst Bacteriol. 1994 Jul;44(3):404–409. doi: 10.1099/00207713-44-3-404. [DOI] [PubMed] [Google Scholar]
  9. Grattard F., Etienne J., Pozzetto B., Tardy F., Gaudin O. G., Fleurette J. Characterization of unrelated strains of Staphylococcus schleiferi by using ribosomal DNA fingerprinting, DNA restriction patterns, and plasmid profiles. J Clin Microbiol. 1993 Apr;31(4):812–818. doi: 10.1128/jcm.31.4.812-818.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Herchline T. E., Ayers L. W. Occurrence of Staphylococcus lugdunensis in consecutive clinical cultures and relationship of isolation to infection. J Clin Microbiol. 1991 Mar;29(3):419–421. doi: 10.1128/jcm.29.3.419-421.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hájek V., Balusek J. Biochemical properties and differentiation of coagulase-positive staphylococci from rooks and gulls. Res Vet Sci. 1988 Mar;44(2):242–246. [PubMed] [Google Scholar]
  12. Hájek V., Balusek J., Horák V., Koukalová D. Characterization of coagulase-positive staphylococci isolated from free-living birds. J Hyg Epidemiol Microbiol Immunol. 1991;35(4):407–418. [PubMed] [Google Scholar]
  13. Hébert G. A., Crowder C. G., Hancock G. A., Jarvis W. R., Thornsberry C. Characteristics of coagulase-negative staphylococci that help differentiate these species and other members of the family Micrococcaceae. J Clin Microbiol. 1988 Oct;26(10):1939–1949. doi: 10.1128/jcm.26.10.1939-1949.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Igimi S., Takahashi E., Mitsuoka T. Staphylococcus schleiferi subsp. coagulans subsp. nov., isolated from the external auditory meatus of dogs with external ear otitis. Int J Syst Bacteriol. 1990 Oct;40(4):409–411. doi: 10.1099/00207713-40-4-409. [DOI] [PubMed] [Google Scholar]
  15. Kanda K., Suzuki E., Hiramatsu K., Oguri T., Miura H., Ezaki T., Yokota T. Identification of a methicillin-resistant strain of Staphylococcus caprae from a human clinical specimen. Antimicrob Agents Chemother. 1991 Jan;35(1):174–176. doi: 10.1128/aac.35.1.174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kloos W. E., Bannerman T. L. Update on clinical significance of coagulase-negative staphylococci. Clin Microbiol Rev. 1994 Jan;7(1):117–140. doi: 10.1128/cmr.7.1.117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kloos W. E., George C. G. Identification of Staphylococcus species and subspecies with the MicroScan Pos ID and Rapid Pos ID panel systems. J Clin Microbiol. 1991 Apr;29(4):738–744. doi: 10.1128/jcm.29.4.738-744.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kloos W. E., Wolfshohl J. F. Staphylococcus cohnii subspecies: Staphylococcus cohnii subsp. cohnii subsp. nov. and Staphylococcus cohnii subsp. urealyticum subsp. nov. Int J Syst Bacteriol. 1991 Apr;41(2):284–289. doi: 10.1099/00207713-41-2-284. [DOI] [PubMed] [Google Scholar]
  19. Kotilainen P., Huovinen P., Eerola E. Application of gas-liquid chromatographic analysis of cellular fatty acids for species identification and typing of coagulase-negative staphylococci. J Clin Microbiol. 1991 Feb;29(2):315–322. doi: 10.1128/jcm.29.2.315-322.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lindsay J. A., Riley T. V. Susceptibility to desferrioxamine: a new test for the identification of Staphylococcus epidermidis. J Med Microbiol. 1991 Jul;35(1):45–48. doi: 10.1099/00222615-35-1-45. [DOI] [PubMed] [Google Scholar]
  21. Lämmler C. Characterization of Staphylococcus hyicus with the ATB 32 Staph system and with conventional tests. J Clin Microbiol. 1991 Jun;29(6):1221–1224. doi: 10.1128/jcm.29.6.1221-1224.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Phillips W. E., Jr, Kloos W. E. Identification of coagulase-positive Staphylococcus intermedius and Staphylococcus hyicus subsp. hyicus isolates from veterinary clinical specimens. J Clin Microbiol. 1981 Dec;14(6):671–673. doi: 10.1128/jcm.14.6.671-673.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pierre J., Gutmann L., Bornet M., Bergogne-Berezin E., Williamson R. Identification of coagulase-negative staphylococci by electrophoretic profile of total proteins and analysis of penicillin-binding proteins. J Clin Microbiol. 1990 Mar;28(3):443–446. doi: 10.1128/jcm.28.3.443-446.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Renneberg J., Rieneck K., Gutschik E. Evaluation of Staph ID 32 system and Staph-Zym system for identification of coagulase-negative staphylococci. J Clin Microbiol. 1995 May;33(5):1150–1153. doi: 10.1128/jcm.33.5.1150-1153.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Shuttleworth R., Colby W. D. Staphylococcus lugdunensis endocarditis. J Clin Microbiol. 1992 Aug;30(8):1948–1952. doi: 10.1128/jcm.30.8.1948-1952.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Talan D. A., Goldstein E. J., Staatz D., Overturf G. D. Staphylococcus intermedius: clinical presentation of a new human dog bite pathogen. Ann Emerg Med. 1989 Apr;18(4):410–413. doi: 10.1016/s0196-0644(89)80582-7. [DOI] [PubMed] [Google Scholar]
  27. Talan D. A., Staatz D., Staatz A., Goldstein E. J., Singer K., Overturf G. D. Staphylococcus intermedius in canine gingiva and canine-inflicted human wound infections: laboratory characterization of a newly recognized zoonotic pathogen. J Clin Microbiol. 1989 Jan;27(1):78–81. doi: 10.1128/jcm.27.1.78-81.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Tanasupawat S., Hashimoto Y., Ezaki T., Kozaki M., Komagata K. Staphylococcus piscifermentans sp. nov., from fermented fish in Thailand. Int J Syst Bacteriol. 1992 Oct;42(4):577–581. doi: 10.1099/00207713-42-4-577. [DOI] [PubMed] [Google Scholar]
  29. Vandenesch F., Eykyn S. J., Bes M., Meugnier H., Fleurette J., Etienne J. Identification and ribotypes of Staphylococcus caprae isolates isolated as human pathogens and from goat milk. J Clin Microbiol. 1995 Apr;33(4):888–892. doi: 10.1128/jcm.33.4.888-892.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Vandenesch F., Lebeau C., Bes M., Lina G., Lina B., Greenland T., Benito Y., Brun Y., Fleurette J., Etienne J. Clotting activity in Staphylococcus schleiferi subspecies from human patients. J Clin Microbiol. 1994 Feb;32(2):388–392. doi: 10.1128/jcm.32.2.388-392.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Webster J. A., Bannerman T. L., Hubner R. J., Ballard D. N., Cole E. M., Bruce J. L., Fiedler F., Schubert K., Kloos W. E. Identification of the Staphylococcus sciuri species group with EcoRI fragments containing rRNA sequences and description of Staphylococcus vitulus sp. nov. Int J Syst Bacteriol. 1994 Jul;44(3):454–460. doi: 10.1099/00207713-44-3-454. [DOI] [PubMed] [Google Scholar]
  32. Welch D. F. Applications of cellular fatty acid analysis. Clin Microbiol Rev. 1991 Oct;4(4):422–438. doi: 10.1128/cmr.4.4.422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. White D. G., Harmon R. J., Langlois B. E. Fluorogenic assay for differentiating Staphylococcus warneri and Staphylococcus hominis strains of bovine origin. J Clin Microbiol. 1990 Mar;28(3):602–602. doi: 10.1128/jcm.28.3.602-.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Zakrzewska-Czerwińska J., Gaszewska-Mastalarz A., Lis B., Gamian A., Mordarski M. Staphylococcus pulvereri sp. nov., isolated from human and animal specimens. Int J Syst Bacteriol. 1995 Jan;45(1):169–172. doi: 10.1099/00207713-45-1-169. [DOI] [PubMed] [Google Scholar]
  35. de la Fuente R., Suarez G., Ruiz Santa Quiteria J. A., Meugnier H., Bes M., Freney J., Fleurette J. Identification of coagulase negative staphylococci isolated from lambs as Staphylococcus caseolyticus. Comp Immunol Microbiol Infect Dis. 1992 Jan;15(1):47–52. doi: 10.1016/0147-9571(92)90101-v. [DOI] [PubMed] [Google Scholar]

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