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. 1996 Dec;34(12):3226–3229. doi: 10.1128/jcm.34.12.3226-3229.1996

Molecular epidemiology provides evidence of genotypic heterogeneity of multidrug-resistant Pseudomonas aeruginosa serotype O:12 outbreak isolates from a pediatric hospital.

E Bingen 1, S Bonacorsi 1, P Rohrlich 1, M Duval 1, S Lhopital 1, N Brahimi 1, E Vilmer 1, R V Goering 1
PMCID: PMC229490  PMID: 8940479

Abstract

Ribotyping randomly amplified polymorphic DNA analysis, and pulsed-field gel electrophoresis were used for the epidemiologic evaluation of eight Pseudomonas aeruginosa O:12 isolates obtained from eight children and two P. aeruginosa O:12 environmental isolates from a hematology ward. Randomly amplified polymorphic DNA analysis and pulsed-field gel electrophoresis were able to discriminate isolates that were indistinguishable by biochemical typing, O serotyping or ribotyping.

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Selected References

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  1. Bingen E. H., Denamur E., Elion J. Use of ribotyping in epidemiological surveillance of nosocomial outbreaks. Clin Microbiol Rev. 1994 Jul;7(3):311–327. doi: 10.1128/cmr.7.3.311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bingen E., Cavé H., Aujard Y., Lambert-Zechovsky N., Desjardins P., Elion J., Denamur E. Molecular analysis of multiply recurrent meningitis due to Escherichia coli K1 in an infant. Clin Infect Dis. 1993 Jan;16(1):82–85. doi: 10.1093/clinids/16.1.82. [DOI] [PubMed] [Google Scholar]
  3. Bingen E., Denamur E., Lambert-Zechovsky N., Aujard Y., Brahimi N., Geslin P., Elion J. Analysis of DNA restriction fragment length polymorphism extends the evidence for breast milk transmission in Streptococcus agalactiae late-onset neonatal infection. J Infect Dis. 1992 Mar;165(3):569–573. doi: 10.1093/infdis/165.3.569. [DOI] [PubMed] [Google Scholar]
  4. Boukadida J., de Montalembert M., Gaillard J. L., Gobin J., Grimont F., Girault D., Véron M., Berche P. Outbreak of gut colonization by Pseudomonas aeruginosa in immunocompromised children undergoing total digestive decontamination: analysis by pulsed-field electrophoresis. J Clin Microbiol. 1991 Sep;29(9):2068–2071. doi: 10.1128/jcm.29.9.2068-2071.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Denamur E., Picard B., Decoux G., Denis J. B., Elion J. The absence of correlation between allozyme and rrn RFLP analysis indicates a high gene flow rate within human clinical Pseudomonas aeruginosa isolates. FEMS Microbiol Lett. 1993 Jul 1;110(3):275–280. doi: 10.1111/j.1574-6968.1993.tb06334.x. [DOI] [PubMed] [Google Scholar]
  6. Denning D. W., Baker C. J., Troup N. J., Tompkins L. S. Restriction endonuclease analysis of human and bovine group B streptococci for epidemiologic study. J Clin Microbiol. 1989 Jun;27(6):1352–1356. doi: 10.1128/jcm.27.6.1352-1356.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grattard F., Gaudin O. G., Pozzetto B., Ros A., Mbida A. D. Genotypic homogeneity of nosocomial Pseudomonas aeruginosa O12 strains demonstrated by analysis of protein profiles, DNA fingerprints and rRNA gene restriction patterns. Eur J Clin Microbiol Infect Dis. 1993 Jan;12(1):57–61. doi: 10.1007/BF01997061. [DOI] [PubMed] [Google Scholar]
  8. Grundmann H., Schneider C., Hartung D., Daschner F. D., Pitt T. L. Discriminatory power of three DNA-based typing techniques for Pseudomonas aeruginosa. J Clin Microbiol. 1995 Mar;33(3):528–534. doi: 10.1128/jcm.33.3.528-534.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kersulyte D., Struelens M. J., Deplano A., Berg D. E. Comparison of arbitrarily primed PCR and macrorestriction (pulsed-field gel electrophoresis) typing of Pseudomonas aeruginosa strains from cystic fibrosis patients. J Clin Microbiol. 1995 Aug;33(8):2216–2219. doi: 10.1128/jcm.33.8.2216-2219.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kobayashi I., Hasegawa M., Miyazaki S., Nishida M., Goto S. In vitro and in vivo changes of serotype in Pseudomonas aeruginosa isolates by anti-pseudomonal drugs. J Antibiot (Tokyo) 1994 Jan;47(1):72–79. doi: 10.7164/antibiotics.47.72. [DOI] [PubMed] [Google Scholar]
  11. Kreiswirth B., Kornblum J., Arbeit R. D., Eisner W., Maslow J. N., McGeer A., Low D. E., Novick R. P. Evidence for a clonal origin of methicillin resistance in Staphylococcus aureus. Science. 1993 Jan 8;259(5092):227–230. doi: 10.1126/science.8093647. [DOI] [PubMed] [Google Scholar]
  12. Lambert-Zechovsky N., Bingen E., Denamur E., Brahimi N., Brun P., Mathieu H., Elion J. Molecular analysis provides evidence for the endogenous origin of bacteremia and meningitis due to Enterobacter cloacae in an infant. Clin Infect Dis. 1992 Jul;15(1):30–32. doi: 10.1093/clinids/15.1.30. [DOI] [PubMed] [Google Scholar]
  13. Liu P. V. Changes in somatic antigens of Pseudomonas aeruginosa induced by bacteriophages. J Infect Dis. 1969 Mar;119(3):237–246. doi: 10.1093/infdis/119.3.237. [DOI] [PubMed] [Google Scholar]
  14. Lányi B., Lantos J. Antigenic changes in Pseudomonas aeruginosa in vivo and after lysogenization in vitro. Acta Microbiol Acad Sci Hung. 1976;23(4):337–351. [PubMed] [Google Scholar]
  15. Ogle J. W., Janda J. M., Woods D. E., Vasil M. L. Characterization and use of a DNA probe as an epidemiological marker for Pseudomonas aeruginosa. J Infect Dis. 1987 Jan;155(1):119–126. doi: 10.1093/infdis/155.1.119. [DOI] [PubMed] [Google Scholar]
  16. Ojeniyi B., Høiby N., Rosdahl V. T. Genome fingerprinting as a typing method used on polyagglutinable Pseudomonas aeruginosa isolates from cystic fibrosis patients. APMIS. 1991 Jun;99(6):492–498. [PubMed] [Google Scholar]
  17. Olson B., Weinstein R. A., Nathan C., Chamberlin W., Kabins S. A. Epidemiology of endemic Pseudomonas aeruginosa: why infection control efforts have failed. J Infect Dis. 1984 Dec;150(6):808–816. doi: 10.1093/infdis/150.6.808. [DOI] [PubMed] [Google Scholar]
  18. Pitt T. L. Epidemiological typing of Pseudomonas aeruginosa. Eur J Clin Microbiol Infect Dis. 1988 Apr;7(2):238–247. doi: 10.1007/BF01963095. [DOI] [PubMed] [Google Scholar]
  19. Pitt T. L., Livermore D. M., Pitcher D., Vatopoulos A. C., Legakis N. J. Multiresistant serotype O 12 Pseudomonas aeruginosa: evidence for a common strain in Europe. Epidemiol Infect. 1989 Dec;103(3):565–576. doi: 10.1017/s095026880003096x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Richard P., Le Floch R., Chamoux C., Pannier M., Espaze E., Richet H. Pseudomonas aeruginosa outbreak in a burn unit: role of antimicrobials in the emergence of multiply resistant strains. J Infect Dis. 1994 Aug;170(2):377–383. doi: 10.1093/infdis/170.2.377. [DOI] [PubMed] [Google Scholar]
  21. Speert D. P., Campbell M. E., Farmer S. W., Volpel K., Joffe A. M., Paranchych W. Use of a pilin gene probe to study molecular epidemiology of Pseudomonas aeruginosa. J Clin Microbiol. 1989 Nov;27(11):2589–2593. doi: 10.1128/jcm.27.11.2589-2593.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tenover F. C., Arbeit R. D., Goering R. V., Mickelsen P. A., Murray B. E., Persing D. H., Swaminathan B. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol. 1995 Sep;33(9):2233–2239. doi: 10.1128/jcm.33.9.2233-2239.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]

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