Abstract
A serological and bacteriological study was performed with sera taken from 2,228 swine from six states in Venezuela. None of the animals were vaccinated against brucellosis, and the prevalence of the disease varied from 5 to 89% on farms located in these states. Our studies indicated that the animals could be categorized into four groups depending on the degree of reactivity in serological tests. Brucella suis biovar 1 was isolated from the lymph nodes, spleens, and semen samples of seropositive animals and identified by oxidative metabolic techniques. B. suis could not be isolated from tissues of seronegative swine even from farms with cases of the disease (detected by serology). Results suggest that, although the immunodiffusion assay using Brucella melitensis B115 polysaccharide B or B. abortus 1119-3 O-polysaccharide could be useful in the detection of active infections, it is perhaps not as sensitive as some of the other standard serological tests used in this study for the detection of swine brucellosis.
Full Text
The Full Text of this article is available as a PDF (154.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bourderioux C., Hubert B., Vol S., Feyt M., Drucker J., Tichet J. Couverture vaccinale et séroprotection vis-à-vis de la rubéole chez les enfants de 11 ans dans le département de l'Indre-et-Loire. Arch Pediatr. 1994 Oct;1(10):941–943. [PubMed] [Google Scholar]
- CAMERON H. S., MEYER M. E. Comparative metabolic studies on the genus Brucella. II. Metabolism of amino acids that occur in the urea cycle. J Bacteriol. 1954 Jan;67(1):34–37. doi: 10.1128/jb.67.1.34-37.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CAMERON H. S., MEYER M. E. Synthesis of amino acids from urea by the genus Brucella. Am J Vet Res. 1955 Jan;16(58):149–151. [PubMed] [Google Scholar]
- Cherwonogrodzky J. W., Nielsen K. H. Brucella abortus 1119-3 O-chain polysaccharide to differentiate sera from B. abortus S-19-vaccinated and field-strain-infected cattle by agar gel immunodiffusion. J Clin Microbiol. 1988 Jun;26(6):1120–1123. doi: 10.1128/jcm.26.6.1120-1123.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Diaz R., Jones L. M., Leong D., Wilson J. B. Surface antigens of smooth brucellae. J Bacteriol. 1968 Oct;96(4):893–901. doi: 10.1128/jb.96.4.893-901.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lord V. R., Cherwonogrodzky J. W. Evaluation of polysaccharide, lipopolysaccharide, and beta-glucan antigens in gel immunodiffusion tests for brucellosis in cattle. Am J Vet Res. 1992 Mar;53(3):389–391. [PubMed] [Google Scholar]
- MEYER M. E., CAMERON H. S. Comparative metabolism of species and types of organisms within the genus Brucella. J Bacteriol. 1959 Jul;78(1):130–136. doi: 10.1128/jb.78.1.130-136.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MEYER M. E., CAMERON H. S. Metabolic characterization of the genus Brucella. I. Statistical evaluation of the oxidative rates by which type I of each species can be identified. J Bacteriol. 1961 Sep;82:387–395. doi: 10.1128/jb.82.3.387-395.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MEYER M. E., CAMERON H. S. Metabolic characterization of the genus Brucella. II. Oxidative metabolic patterns of the described biotypes. J Bacteriol. 1961 Sep;82:396–400. doi: 10.1128/jb.82.3.396-400.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MEYER M. E., MORGAN W. J. Metabolic characterization of Brucella strains that show conflicting identity by biochemical and serological methods. Bull World Health Organ. 1962;26:823–827. [PMC free article] [PubMed] [Google Scholar]
- Matyas Z., Fujikura T. Brucellosis as a world problem. Dev Biol Stand. 1984;56:3–20. [PubMed] [Google Scholar]