Abstract
For 275 samples tested for herpes simplex virus (HSV), cytospin-enhanced direct immunofluorescence using Chemicon HSV monoclonal antibodies identified 80 (95%) and culture identified 77 (92%) of 84 confirmed positive specimens. Cytospin-prepared slides contained a greater number of total cells than standard cell spots, resulting in fewer inadequate cell smears and a higher HSV detection rate.
Full Text
The Full Text of this article is available as a PDF (141.4 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chapin-Robertson K., Dahlberg S. E., Edberg S. C. Clinical and laboratory analyses of cytospin-prepared Gram stains for recovery and diagnosis of bacteria from sterile body fluids. J Clin Microbiol. 1992 Feb;30(2):377–380. doi: 10.1128/jcm.30.2.377-380.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Choi H. S., Anderson P. J. Diagnostic cytology of cerebrospinal fluid by the cytocentrifuge method. Am J Clin Pathol. 1979 Dec;72(6):931–943. doi: 10.1093/ajcp/72.6.931. [DOI] [PubMed] [Google Scholar]
- Fung J. C., Shanley J., Tilton R. C. Comparison of the detection of herpes simplex virus in direct clinical specimens with herpes simplex virus-specific DNA probes and monoclonal antibodies. J Clin Microbiol. 1985 Nov;22(5):748–753. doi: 10.1128/jcm.22.5.748-753.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goldstein L. C., Corey L., McDougall J. K., Tolentino E., Nowinski R. C. Monoclonal antibodies to herpes simplex viruses: use in antigenic typing and rapid diagnosis. J Infect Dis. 1983 May;147(5):829–837. doi: 10.1093/infdis/147.5.829. [DOI] [PubMed] [Google Scholar]
- Hoffmann B. E., Jungkind D. L., Haller G. J., Sharrar R., Baker R. A., Weisberg M. Evaluation of two rapid methods for the detection of herpes simplex virus antigen in patient specimens. Ann Clin Lab Sci. 1985 Sep-Oct;15(5):418–427. [PubMed] [Google Scholar]
- Landry M. L., Ferguson D. Comparison of quantitative cytomegalovirus antigenemia assay with culture methods and correlation with clinical disease. J Clin Microbiol. 1993 Nov;31(11):2851–2856. doi: 10.1128/jcm.31.11.2851-2856.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martinazzi M. A slide centrifugation technic for concentrating blood leukocytes and nucleated cells from bone marrow blood. Am J Clin Pathol. 1971 Dec;56(6):719–722. doi: 10.1093/ajcp/56.6.719. [DOI] [PubMed] [Google Scholar]
- Moseley R. C., Corey L., Benjamin D., Winter C., Remington M. L. Comparison of viral isolation, direct immunofluorescence, and indirect immunoperoxidase techniques for detection of genital herpes simplex virus infection. J Clin Microbiol. 1981 May;13(5):913–918. doi: 10.1128/jcm.13.5.913-918.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pouletty P., Chomel J. J., Thouvenot D., Catalan F., Rabillon V., Kadouche J. Detection of herpes simplex virus in direct specimens by immunofluorescence assay using a monoclonal antibody. J Clin Microbiol. 1987 May;25(5):958–959. doi: 10.1128/jcm.25.5.958-959.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schmidt N. J., Dennis J., Devlin V., Gallo D., Mills J. Comparison of direct immunofluorescence and direct immunoperoxidase procedures for detection of herpes simplex virus antigen in lesion specimens. J Clin Microbiol. 1983 Aug;18(2):445–448. doi: 10.1128/jcm.18.2.445-448.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Volpi A., Lakeman A. D., Pereira L., Stagno S. Monoclonal antibodies for rapid diagnosis and typing of genital herpes infections during pregnancy. Am J Obstet Gynecol. 1983 Aug 1;146(7):813–815. doi: 10.1016/0002-9378(83)91083-9. [DOI] [PubMed] [Google Scholar]