Abstract
Duplicate blood samples collected in EDTA and acid-citrate-dextrose (ACD) were compared by cytomegalovirus (CMV) pp65 antigenemia and CMV infectivity on the day of sample collection and after 1 and 2 days of storage at 4 degrees C. No significant difference was detected between EDTA and ACD. However, CMV antigenemia was more sensitive than culture at all time points tested.
Full Text
The Full Text of this article is available as a PDF (138.2 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Boeckh M., Woogerd P. M., Stevens-Ayers T., Ray C. G., Bowden R. A. Factors influencing detection of quantitative cytomegalovirus antigenemia. J Clin Microbiol. 1994 Mar;32(3):832–834. doi: 10.1128/jcm.32.3.832-834.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gerna G., Zipeto D., Parea M., Revello M. G., Silini E., Percivalle E., Zavattoni M., Grossi P., Milanesi G. Monitoring of human cytomegalovirus infections and ganciclovir treatment in heart transplant recipients by determination of viremia, antigenemia, and DNAemia. J Infect Dis. 1991 Sep;164(3):488–498. doi: 10.1093/infdis/164.3.488. [DOI] [PubMed] [Google Scholar]
- Landry M. L., Ferguson D., Cohen S., Huber K., Wetherill P. Effect of delayed specimen processing on cytomegalovirus antigenemia test results. J Clin Microbiol. 1995 Jan;33(1):257–259. doi: 10.1128/jcm.33.1.257-259.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Landry M. L., Ferguson D. Comparison of quantitative cytomegalovirus antigenemia assay with culture methods and correlation with clinical disease. J Clin Microbiol. 1993 Nov;31(11):2851–2856. doi: 10.1128/jcm.31.11.2851-2856.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McCullough J., Carter S. J., Quie P. G. Effects of anticoagulants and storage on granulocyte function in bank blood. Blood. 1974 Feb;43(2):207–217. [PubMed] [Google Scholar]
- Niubò J., Pérez J. L., Carvajal A., Ardanuy C., Martín R. Effect of delayed processing of blood samples on performance of cytomegalovirus antigenemia assay. J Clin Microbiol. 1994 Apr;32(4):1119–1120. doi: 10.1128/jcm.32.4.1119-1120.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Storch G. A., Gaudreault-Keener M., Welby P. C. Comparison of heparin and EDTA transport tubes for detection of cytomegalovirus in leukocytes by shell vial assay, pp65 antigenemia assay, and PCR. J Clin Microbiol. 1994 Oct;32(10):2581–2583. doi: 10.1128/jcm.32.10.2581-2583.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- The T. H., van der Ploeg M., van den Berg A. P., Vlieger A. M., van der Giessen M., van Son W. J. Direct detection of cytomegalovirus in peripheral blood leukocytes--a review of the antigenemia assay and polymerase chain reaction. Transplantation. 1992 Aug;54(2):193–198. doi: 10.1097/00007890-199208000-00001. [DOI] [PubMed] [Google Scholar]