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. 1997 Jan;35(1):325–327. doi: 10.1128/jcm.35.1.325-327.1997

Use of pulsed-field gel electrophoresis to investigate an outbreak of Serratia marcescens.

Z Y Shi 1, P Y Liu 1, Y J Lau 1, Y H Lin 1, B S HU 1
PMCID: PMC229571  PMID: 8968940

Abstract

Pulsed-field gel electrophoresis (PFGE) typing was applied to the epidemiological investigation of 20 Serratia marcescens isolates collected from urine specimens of 17 patients and three urinals over a 2-month period. Twenty-five epidemiologically unrelated strains were also tested to determine the discriminatory power of PFGE. The PFGE fingerprints of each isolate were consistent in three different tests. The 20 outbreak isolates had an identical PFGE fingerprint pattern, while the epidemiologically unrelated strains demonstrated unique PFGE fingerprint patterns. The source of the outbreak was inadequately disinfected urinals. We conclude that PFGE served as a highly discriminatory and reproducible method for the epidemiological investigation of the outbreak of S. marcescens infection addressed by this study.

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Selected References

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  1. Alonso R., Aucken H. M., Perez-Diaz J. C., Cookson B. D., Baquero F., Pitt T. L. Comparison of serotype, biotype and bacteriocin type with rDNA RFLP patterns for the type identification of Serratia marcescens. Epidemiol Infect. 1993 Aug;111(1):99–107. doi: 10.1017/s0950268800056727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arbeit R. D., Arthur M., Dunn R., Kim C., Selander R. K., Goldstein R. Resolution of recent evolutionary divergence among Escherichia coli from related lineages: the application of pulsed field electrophoresis to molecular epidemiology. J Infect Dis. 1990 Feb;161(2):230–235. doi: 10.1093/infdis/161.2.230. [DOI] [PubMed] [Google Scholar]
  3. Bingen E. H., Mariani-Kurkdjian P., Lambert-Zechovsky N. Y., Desjardins P., Denamur E., Aujard Y., Vilmer E., Elion J. Ribotyping provides efficient differentiation of nosocomial Serratia marcescens isolates in a pediatric hospital. J Clin Microbiol. 1992 Aug;30(8):2088–2091. doi: 10.1128/jcm.30.8.2088-2091.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chetoui H., Delhalle E., Osterrieth P., Rousseaux D. Ribotyping for use in studying molecular epidemiology of Serratia marcescens: comparison with biotyping. J Clin Microbiol. 1995 Oct;33(10):2637–2642. doi: 10.1128/jcm.33.10.2637-2642.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Debast S. B., Melchers W. J., Voss A., Hoogkamp-Korstanje J. A., Meis J. F. Epidemiological survey of an outbreak of multiresistant Serratia marcescens by PCR-fingerprinting. Infection. 1995 Sep-Oct;23(5):267–271. doi: 10.1007/BF01716283. [DOI] [PubMed] [Google Scholar]
  6. Gargallo-Viola D. Enzyme polymorphism, prodigiosin production, and plasmid fingerprints in clinical and naturally occurring isolates of Serratia marcescens. J Clin Microbiol. 1989 May;27(5):860–868. doi: 10.1128/jcm.27.5.860-868.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grimont P. A., Grimont F., Le Minor S., Davis B., Pigache F. Compatible results obtained from biotyping and serotyping in Serratia marcescens. J Clin Microbiol. 1979 Oct;10(4):425–432. doi: 10.1128/jcm.10.4.425-432.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hamilton R. L., Brown W. J. Bacteriophage typing of clinically isolated Serratia marcescens. Appl Microbiol. 1972 Dec;24(6):899–906. doi: 10.1128/am.24.6.899-906.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hermans P. W., Sluijter M., Hoogenboezem T., Heersma H., van Belkum A., de Groot R. Comparative study of five different DNA fingerprint techniques for molecular typing of Streptococcus pneumoniae strains. J Clin Microbiol. 1995 Jun;33(6):1606–1612. doi: 10.1128/jcm.33.6.1606-1612.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Liu P. Y., Lau Y. J., Hu B. S., Shir J. M., Cheung M. H., Shi Z. Y., Tsai W. S. Use of PCR to study epidemiology of Serratia marcescens isolates in nosocomial infection. J Clin Microbiol. 1994 Aug;32(8):1935–1938. doi: 10.1128/jcm.32.8.1935-1938.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Liu P. Y., Lau Y. J., Hu B. S., Shyr J. M., Shi Z. Y., Tsai W. S., Lin Y. H., Tseng C. Y. Analysis of clonal relationships among isolates of Shigella sonnei by different molecular typing methods. J Clin Microbiol. 1995 Jul;33(7):1779–1783. doi: 10.1128/jcm.33.7.1779-1783.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MacPherson J. M., Eckstein P. E., Scoles G. J., Gajadhar A. A. Variability of the random amplified polymorphic DNA assay among thermal cyclers, and effects of primer and DNA concentration. Mol Cell Probes. 1993 Aug;7(4):293–299. doi: 10.1006/mcpr.1993.1043. [DOI] [PubMed] [Google Scholar]
  13. Okuda T., Endo N., Osada Y., Zen-Yoji H. Outbreak of nosocomial urinary tract infections caused by Serratia marcescens. J Clin Microbiol. 1984 Oct;20(4):691–695. doi: 10.1128/jcm.20.4.691-695.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pitt T. L. State of the art: typing of Serratia marcescens. J Hosp Infect. 1982 Mar;3(1):9–14. doi: 10.1016/0195-6701(82)90026-3. [DOI] [PubMed] [Google Scholar]
  15. Sader H. S., Perl T. M., Hollis R. J., Divishek D., Herwaldt L. A., Jones R. N. Nosocomial transmission of Serratia odorifera biogroup. 2: Case report demonstration by macrorestriction analysis of chromosomal DNA using pulsed-field gel electrophoresis. Infect Control Hosp Epidemiol. 1994 Jun;15(6):390–393. doi: 10.1086/646936. [DOI] [PubMed] [Google Scholar]
  16. Schaberg D. R., Alford R. H., Anderson R., Farmer J. J., 3rd, Melly M. A., Schaffner W. An outbreak of nosocomial infection due to multiply resistant Serratia marcescens: evidence of interhospital spread. J Infect Dis. 1976 Aug;134(2):181–188. doi: 10.1093/infdis/134.2.181. [DOI] [PubMed] [Google Scholar]
  17. Sproat D., Brown A. Bacteriocin typing of Serratia marcescens. A simplified system. Am J Clin Pathol. 1979 Feb;71(2):172–176. doi: 10.1093/ajcp/71.2.172. [DOI] [PubMed] [Google Scholar]
  18. Tenover F. C., Arbeit R. D., Goering R. V., Mickelsen P. A., Murray B. E., Persing D. H., Swaminathan B. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol. 1995 Sep;33(9):2233–2239. doi: 10.1128/jcm.33.9.2233-2239.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Welsh J., McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 1990 Dec 25;18(24):7213–7218. doi: 10.1093/nar/18.24.7213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yu V. L. Serratia marcescens: historical perspective and clinical review. N Engl J Med. 1979 Apr 19;300(16):887–893. doi: 10.1056/NEJM197904193001604. [DOI] [PubMed] [Google Scholar]
  21. van Belkum A., Bax R., Prevost G. Comparison of four genotyping assays for epidemiological study of methicillin-resistant Staphylococcus aureus. Eur J Clin Microbiol Infect Dis. 1994 May;13(5):420–424. doi: 10.1007/BF01972002. [DOI] [PubMed] [Google Scholar]

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