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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1997 Feb;35(2):369–373. doi: 10.1128/jcm.35.2.369-373.1997

Characterization of Aeromonas spp. isolated from humans with diarrhea, from healthy controls, and from surface water in Bangladesh.

I Kühn 1, M J Albert 1, M Ansaruzzaman 1, N A Bhuiyan 1, S A Alabi 1, M S Islam 1, P K Neogi 1, G Huys 1, P Janssen 1, K Kersters 1, R Möllby 1
PMCID: PMC229582  PMID: 9003598

Abstract

Aeromonas isolates from patients with diarrhea in Bangladesh (n = 69), from healthy controls (n = 11), and from surface water (n = 40) were analyzed with respect to their hybridization groups (HGs) by the aid of fatty acid methyl ester (FAME) characterization and DNA fingerprinting by AFLP, biochemical phenotypes (Phe-nePlate [PhP] types), and the production of hemolysin and cytotoxin. The aim of the investigation was to find out whether certain strains carrying virulence factors predominated among patient isolates. According to FAME and/or AFLP analysis, most human isolates were allocated to DNA HGs 4 (Aeromonas caviae) and 1 (A. hydrophila). Most environmental strains were allocated to HG8 (A. veronii biogroup sobria) and HG4 (A. caviae), and only one was of HG1. According to PhP typing, the diversity among patient isolates was lower than that among other strains, and two dominating PhP types (types BD-1 and BD-2) were identified in 29 and 30% of the patient isolates, respectively. PhP type BD-1 was also common among the environmental isolates, whereas PhP type BD-2 was only identified in two of the other isolates. Twenty-five of 26 isolates belonging to HG1 were of the same PhP type (BD-2), whereas isolates of other common HGs were more diverse according to their PhP types. Hemolytic and cytotoxin-producing strains occurred more frequently among the environmental isolates than among patient isolates. However, the hemolytic and cytotoxic activities among human isolates was strongly correlated to the HG1/BD-2 type, which, in addition, showed high cytotoxin titers (median values, 1/512 compared to 1/128 for cytotoxin-positive isolates belonging to other types). Thus, the HG1/BD-2 type may represent a pathogenic A. hydrophila type that is able to produce diarrhea in humans.

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Selected References

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  1. Araujo R. M., Pares R., Lucena F. The effect of terrestrial effluents on the incidence of Aeromonas spp. in coastal waters. J Appl Bacteriol. 1990 Sep;69(3):439–444. doi: 10.1111/j.1365-2672.1990.tb01535.x. [DOI] [PubMed] [Google Scholar]
  2. Aziz K. M., Rahim Z., Faruqe A. S., Huq S., Eusof A. Aeromonas hydrophila: its isolation from acute diarrhoeal illness in rural Bangladesh. Bangladesh Med Res Counc Bull. 1986 Dec;12(2):49–58. [PubMed] [Google Scholar]
  3. Begue R. E., Castellares G., Hayashi K. E., Ruiz R., Meza R., English C. K., Gotuzzo E., Sanchez J. L., Oberst R. Diarrheal disease in Peru after the introduction of cholera. Am J Trop Med Hyg. 1994 Nov;51(5):585–589. doi: 10.4269/ajtmh.1994.51.585. [DOI] [PubMed] [Google Scholar]
  4. Carnahan A. M., Behram S., Joseph S. W. Aerokey II: a flexible key for identifying clinical Aeromonas species. J Clin Microbiol. 1991 Dec;29(12):2843–2849. doi: 10.1128/jcm.29.12.2843-2849.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Deodhar L. P., Saraswathi K., Varudkar A. Aeromonas spp. and their association with human diarrheal disease. J Clin Microbiol. 1991 May;29(5):853–856. doi: 10.1128/jcm.29.5.853-856.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gentry M. K., Dalrymple J. M. Quantitative microtiter cytotoxicity assay for Shigella toxin. J Clin Microbiol. 1980 Sep;12(3):361–366. doi: 10.1128/jcm.12.3.361-366.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gray S. J., Stickler D. J., Bryant T. N. The incidence of virulence factors in mesophilic Aeromonas species isolated from farm animals and their environment. Epidemiol Infect. 1990 Oct;105(2):277–294. doi: 10.1017/s0950268800047889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Havelaar A. H., Schets F. M., van Silfhout A., Jansen W. H., Wieten G., van der Kooij D. Typing of Aeromonas strains from patients with diarrhoea and from drinking water. J Appl Bacteriol. 1992 May;72(5):435–444. doi: 10.1111/j.1365-2672.1992.tb01857.x. [DOI] [PubMed] [Google Scholar]
  9. Hossain M. A., Rahman K. M., Asna S. M., Rahim Z., Hussain T., Miah M. R. Incidence of Aeromonas isolated from diarrhoeal children and study of some virulence factors in the isolates. Bangladesh Med Res Counc Bull. 1992 Dec;18(2):61–67. [PubMed] [Google Scholar]
  10. Houston C. W., Chopra A. K., Rose J. M., Kurosky A. Review of Aeromonas enterotoxins. Experientia. 1991 May 15;47(5):424–426. [PubMed] [Google Scholar]
  11. Huys G., Coopman R., Janssen P., Kersters K. High-resolution genotypic analysis of the genus Aeromonas by AFLP fingerprinting. Int J Syst Bacteriol. 1996 Apr;46(2):572–580. doi: 10.1099/00207713-46-2-572. [DOI] [PubMed] [Google Scholar]
  12. Huys G., Kersters I., Vancanneyt M., Coopman R., Janssen P., Kersters K. Diversity of Aeromonas sp. in Flemish drinking water production plants as determined by gas-liquid chromatographic analysis of cellular fatty acid methyl esters (FAMEs). J Appl Bacteriol. 1995 Apr;78(4):445–455. doi: 10.1111/j.1365-2672.1995.tb03432.x. [DOI] [PubMed] [Google Scholar]
  13. Hänninen M. L., Salmi S., Mattila L., Taipalinen R., Siitonen A. Association of Aeromonas spp. with travellers' diarrhoea in Finland. J Med Microbiol. 1995 Jan;42(1):26–31. doi: 10.1099/00222615-42-1-26. [DOI] [PubMed] [Google Scholar]
  14. Janda J. M., Guthertz L. S., Kokka R. P., Shimada T. Aeromonas species in septicemia: laboratory characteristics and clinical observations. Clin Infect Dis. 1994 Jul;19(1):77–83. doi: 10.1093/clinids/19.1.77. [DOI] [PubMed] [Google Scholar]
  15. Janda J. M., Kokka R. P. The pathogenicity of Aeromonas strains relative to genospecies and phenospecies identification. FEMS Microbiol Lett. 1991 Dec 15;69(1):29–33. doi: 10.1111/j.1574-6968.1991.tb05120.x. [DOI] [PubMed] [Google Scholar]
  16. Kelly K. A., Koehler J. M., Ashdown L. R. Spectrum of extraintestinal disease due to Aeromonas species in tropical Queensland, Australia. Clin Infect Dis. 1993 Apr;16(4):574–579. doi: 10.1093/clind/16.4.574. [DOI] [PubMed] [Google Scholar]
  17. Kirov S. M. The public health significance of Aeromonas spp. in foods. Int J Food Microbiol. 1993 Dec;20(4):179–198. doi: 10.1016/0168-1605(93)90164-c. [DOI] [PubMed] [Google Scholar]
  18. Kokka R. P., Janda J. M., Oshiro L. S., Altwegg M., Shimada T., Sakazaki R., Brenner D. J. Biochemical and genetic characterization of autoagglutinating phenotypes of Aeromonas species associated with invasive and noninvasive disease. J Infect Dis. 1991 Apr;163(4):890–894. doi: 10.1093/infdis/163.4.890. [DOI] [PubMed] [Google Scholar]
  19. Krovacek K., Pasquale V., Baloda S. B., Soprano V., Conte M., Dumontet S. Comparison of putative virulence factors in Aeromonas hydrophila strains isolated from the marine environment and human diarrheal cases in southern Italy. Appl Environ Microbiol. 1994 Apr;60(4):1379–1382. doi: 10.1128/aem.60.4.1379-1382.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kühn I., Allestam G., Stenström T. A., Möllby R. Biochemical fingerprinting of water coliform bacteria, a new method for measuring phenotypic diversity and for comparing different bacterial populations. Appl Environ Microbiol. 1991 Nov;57(11):3171–3177. doi: 10.1128/aem.57.11.3171-3177.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Majeed K. N., Macrae I. C. Cytotoxic and haemagglutinating activities of motile Aeromonas species. J Med Microbiol. 1994 Mar;40(3):188–193. doi: 10.1099/00222615-40-3-188. [DOI] [PubMed] [Google Scholar]
  22. Muñoz P., Fernández-Baca V., Peláez T., Sánchez R., Rodríguez-Créixems M., Bouza E. Aeromonas peritonitis. Clin Infect Dis. 1994 Jan;18(1):32–37. [PubMed] [Google Scholar]
  23. Namdari H., Bottone E. J. Cytotoxin and enterotoxin production as factors delineating enteropathogenicity of Aeromonas caviae. J Clin Microbiol. 1990 Aug;28(8):1796–1798. doi: 10.1128/jcm.28.8.1796-1798.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ogunsanya T. I., Rotimi V. O., Adenuga A. A study of the aetiological agents of childhood diarrhoea in Lagos, Nigeria. J Med Microbiol. 1994 Jan;40(1):10–14. doi: 10.1099/00222615-40-1-10. [DOI] [PubMed] [Google Scholar]
  25. Osterhout G. J., Shull V. H., Dick J. D. Identification of clinical isolates of gram-negative nonfermentative bacteria by an automated cellular fatty acid identification system. J Clin Microbiol. 1991 Sep;29(9):1822–1830. doi: 10.1128/jcm.29.9.1822-1830.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Pin C., Marín M. L., Selgas D., García M. L., Tormo J., Casas C. Differences in production of several extracellular virulence factors in clinical and food Aeromonas spp. strains. J Appl Bacteriol. 1995 Feb;78(2):175–179. doi: 10.1111/j.1365-2672.1995.tb02839.x. [DOI] [PubMed] [Google Scholar]
  27. Singh D. V., Sanyal S. C. Haemolysin and enterotoxin production by Aeromonas caviae isolated from diarrhoeal patients, fish and environment. J Diarrhoeal Dis Res. 1992 Mar;10(1):16–20. [PubMed] [Google Scholar]
  28. Vos P., Hogers R., Bleeker M., Reijans M., van de Lee T., Hornes M., Frijters A., Pot J., Peleman J., Kuiper M. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 1995 Nov 11;23(21):4407–4414. doi: 10.1093/nar/23.21.4407. [DOI] [PMC free article] [PubMed] [Google Scholar]

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