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. 1997 Feb;35(2):492–494. doi: 10.1128/jcm.35.2.492-494.1997

Mutations in the rpoB gene of Mycobacterium tuberculosis that interfere with PCR-single-strand conformation polymorphism analysis for rifampin susceptibility testing.

B J Kim 1, S Y Kim 1, B H Park 1, M A Lyu 1, I K Park 1, G H Bai 1, S J Kim 1, C Y Cha 1, Y H Kook 1
PMCID: PMC229609  PMID: 9003625

Abstract

Rifampin susceptibility of 32 rifampin-resistant and 26 rifampin-susceptible Mycobacterium tuberculosis strains was analyzed by PCR-single-strand conformation polymorphism (SSCP) and DNA sequencing within the 157-bp region of the rpoB gene (Ala500 to Val550). Two false-positive PCR-SSCP results were observed among the susceptible strains due to the silent mutation Gln513 (CAA-->CAG) and the deletion mutation Thr508 and Ser509. Another silent mutation [Leu511 (CTG-->CTA)], combined with the mutation Ser531-->Leu, was observed in a resistant strain. These results suggest that to rule out false-positive PCR-SSCP results, sequencing of the target DNA is required.

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Selected References

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