Abstract
Fifteen horses were experimentally infected with African horse sickness virus (AHSV) serotype 4. To learn more about the time course of production and specificity of AHSV-specific antibodies, sera were analyzed by immunoblot analysis. Only animals that survived for more than 9 days were able to develop a humoral immune response detectable by immunoblotting. The earliest serological markers corresponded mainly to VP5, VP6, and NS2 and to a lesser extent to VP3, NS1, and NS3. Neutralizing antibodies to VP2 were not detected by immunoblotting, suggesting that they are mostly conformation dependent. VP7-specific antibodies were detected later in infection. These results make NS2 and VP6 the most attractive candidates for the rapid diagnosis of the infection.
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Selected References
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- Adkison M. A., Stott J. L., Osburn B. I. Temporal development of bluetongue virus protein-specific antibody in sheep following natural infection. Vet Microbiol. 1988 Mar;16(3):231–241. doi: 10.1016/0378-1135(88)90027-2. [DOI] [PubMed] [Google Scholar]
- Bremer C. W., Huismans H., Van Dijk A. A. Characterization and cloning of the African horsesickness virus genome. J Gen Virol. 1990 Apr;71(Pt 4):793–799. doi: 10.1099/0022-1317-71-4-793. [DOI] [PubMed] [Google Scholar]
- Bremer C. W., du Plessis D. H., van Dijk A. A. Baculovirus expression of non-structural protein NS2 and core protein VP7 of African horsesickness virus serotype 3 and their use as antigens in an indirect ELISA. J Virol Methods. 1994 Jul;48(2-3):245–256. doi: 10.1016/0166-0934(94)90123-6. [DOI] [PubMed] [Google Scholar]
- Burrage T. G., Trevejo R., Stone-Marschat M., Laegreid W. W. Neutralizing epitopes of African horsesickness virus serotype 4 are located on VP2. Virology. 1993 Oct;196(2):799–803. doi: 10.1006/viro.1993.1537. [DOI] [PubMed] [Google Scholar]
- Chuma T., Le Blois H., Sánchez-Vizcaíno J. M., Diaz-Laviada M., Roy P. Expression of the major core antigen VP7 of African horsesickness virus by a recombinant baculovirus and its use as a group-specific diagnostic reagent. J Gen Virol. 1992 Apr;73(Pt 4):925–931. doi: 10.1099/0022-1317-73-4-925. [DOI] [PubMed] [Google Scholar]
- Hamblin C., Mertens P. P., Mellor P. S., Burroughs J. N., Crowther J. R. A serogroup specific enzyme-linked immunosorbent assay for the detection and identification of African horse sickness viruses. J Virol Methods. 1991 Feb-Mar;31(2-3):285–292. doi: 10.1016/0166-0934(91)90166-w. [DOI] [PubMed] [Google Scholar]
- Laviada M. D., Arias M., Sánchez-Vizcaíno J. M. Characterization of African horsesickness virus serotype 4-induced polypeptides in Vero cells and their reactivity in Western immunoblotting. J Gen Virol. 1993 Jan;74(Pt 1):81–87. doi: 10.1099/0022-1317-74-1-81. [DOI] [PubMed] [Google Scholar]
- Laviada M. D., Babín M., Dominguez J., Sánchez-Vizcaíno J. M. Detection of African horsesickness virus in infected spleens by a sandwich ELISA using two monoclonal antibodies specific for VP7. J Virol Methods. 1992 Aug;38(2):229–242. doi: 10.1016/0166-0934(92)90113-r. [DOI] [PubMed] [Google Scholar]
- Laviada M. D., Roy P., Sánchez-Vizcaíno J. M., Casal J. I. The use of African horse sickness virus NS3 protein, expressed in bacteria, as a marker to differentiate infected from vaccinated horses. Virus Res. 1995 Oct;38(2-3):205–218. doi: 10.1016/0168-1702(95)00061-t. [DOI] [PubMed] [Google Scholar]
- Martinez-Torrecuadrada J. L., Iwata H., Venteo A., Casal I., Roy P. Expression and characterization of the two outer capsid proteins of African horsesickness virus: the role of VP2 in virus neutralization. Virology. 1994 Jul;202(1):348–359. doi: 10.1006/viro.1994.1351. [DOI] [PubMed] [Google Scholar]
- Martínez-Torrecuadrada J. L., Díaz-Laviada M., Roy P., Sánchez C., Vela C., Sánchez-Vizcaíno J. M., Casal J. I. Full protection against African horsesickness (AHS) in horses induced by baculovirus-derived AHS virus serotype 4 VP2, VP5 and VP7. J Gen Virol. 1996 Jun;77(Pt 6):1211–1221. doi: 10.1099/0022-1317-77-6-1211. [DOI] [PubMed] [Google Scholar]
- Mellor P. S. African horse sickness: transmission and epidemiology. Vet Res. 1993;24(2):199–212. [PubMed] [Google Scholar]
- Ranz A. I., Miguet J. G., Anaya C., Venteo A., Cortés E., Vela C., Sanz A. Diagnostic methods for African horsesickness virus using monoclonal antibodies to structural and non-structural proteins. Vet Microbiol. 1992 Nov;33(1-4):143–153. doi: 10.1016/0378-1135(92)90042-r. [DOI] [PubMed] [Google Scholar]
- Rodriguez M., Hooghuis H., Castaño M. African horse sickness in Spain. Vet Microbiol. 1992 Nov;33(1-4):129–142. doi: 10.1016/0378-1135(92)90041-q. [DOI] [PubMed] [Google Scholar]
- Roy P., Adachi A., Urakawa T., Booth T. F., Thomas C. P. Identification of bluetongue virus VP6 protein as a nucleic acid-binding protein and the localization of VP6 in virus-infected vertebrate cells. J Virol. 1990 Jan;64(1):1–8. doi: 10.1128/jvi.64.1.1-8.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roy P., Mertens P. P., Casal I. African horse sickness virus structure. Comp Immunol Microbiol Infect Dis. 1994 Aug-Nov;17(3-4):243–273. doi: 10.1016/0147-9571(94)90046-9. [DOI] [PubMed] [Google Scholar]