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. 1997 Feb;35(2):531–535. doi: 10.1128/jcm.35.2.531-535.1997

Serologic markers in early stages of African horse sickness virus infection.

J L Martínez-Torrecuadrada 1, M Díaz-Laviada 1, P Roy 1, C Sánchez 1, C Vela 1, J M Sánchez-Vizcaíno 1, J I Casal 1
PMCID: PMC229621  PMID: 9003637

Abstract

Fifteen horses were experimentally infected with African horse sickness virus (AHSV) serotype 4. To learn more about the time course of production and specificity of AHSV-specific antibodies, sera were analyzed by immunoblot analysis. Only animals that survived for more than 9 days were able to develop a humoral immune response detectable by immunoblotting. The earliest serological markers corresponded mainly to VP5, VP6, and NS2 and to a lesser extent to VP3, NS1, and NS3. Neutralizing antibodies to VP2 were not detected by immunoblotting, suggesting that they are mostly conformation dependent. VP7-specific antibodies were detected later in infection. These results make NS2 and VP6 the most attractive candidates for the rapid diagnosis of the infection.

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Selected References

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