Abstract
We previously reported an inhibitory effect on Mycobacterium avium-M. intracellulare (MAI) when blood collected and processed with the Isolator system was placed in BACTEC 12B bottles for radiometric monitoring. We sought to identify the specific component(s) of the Isolator lysis-anticoagulant reagent (LAR) responsible for the inhibitory effect. We added the three components of the LAR, saponin, polyanetholesulfonate, and polypropylene glycol (PPG), to triplicate sets of BACTEC bottles separately and in various combinations. These bottles were then seeded with 10(2) CFU of MAI. The growth index (GI) was observed over a 42-day period and was compared with the GI for a growth control bottle set with no reagents and with the GI for a bottle set containing an equivalent volume of LAR. Growth in all growth control bottles and those bottles containing no PPG reached the maximum GI (GI = 999) within 8 to 10 days. Growth in bottles containing PPG never reached the maximum GI before day 14. In addition, the GIs of one bottle containing all three reagent components as well as all of the bottles containing actual Isolator LAR failed to reach the maximum within 42 days. Our data suggest that PPG is the main cause of the inhibitory effect, but that a secondary synergistic interaction between all three of the reagents may also be present.
Full Text
The Full Text of this article is available as a PDF (63.5 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chidiac C., Mouton Y. Infections à mycobactéries atypiques au cours du SIDA. Place et diagnostic. Presse Med. 1993 Oct 9;22(30):1405–1412. [PubMed] [Google Scholar]
- Doern G. V., Westerling J. A. Optimum recovery of Mycobacterium avium complex from blood specimens of human immunodeficiency virus-positive patients by using small volumes of isolator concentrate inoculated into BACTEC 12B bottles. J Clin Microbiol. 1994 Oct;32(10):2576–2577. doi: 10.1128/jcm.32.10.2576-2577.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gill V. J., Park C. H., Stock F., Gosey L. L., Witebsky F. G., Masur H. Use of lysis-centrifugation (isolator) and radiometric (BACTEC) blood culture systems for the detection of mycobacteremia. J Clin Microbiol. 1985 Oct;22(4):543–546. doi: 10.1128/jcm.22.4.543-546.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gill V. J., Stock F. Detection of Mycobacterium avium-Mycobacterium intracellulare in blood cultures using concentrated and unconcentrated blood in conjunction with a radiometric detection system. Diagn Microbiol Infect Dis. 1987 Feb;6(2):119–123. doi: 10.1016/0732-8893(87)90095-2. [DOI] [PubMed] [Google Scholar]
- Pierre-Audigier C. Techniques biologiques récentes pour le diagnostic des infections à mycobactéries. Presse Med. 1994 Apr 9;23(14):665–670. [PubMed] [Google Scholar]
- Shanson D. C., Dryden M. S. Comparison of methods for isolating Mycobacterium avium-intracellulare from blood of patients with AIDS. J Clin Pathol. 1988 Jun;41(6):687–690. doi: 10.1136/jcp.41.6.687. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wasilauskas B. L., Morrell R., Jr Optimum recovery of Mycobacterium avium complex from blood specimens of human immunodeficiency virus-positive patients by using small volumes of isolator concentrate inoculated into BACTEC 12B bottles. J Clin Microbiol. 1995 Mar;33(3):784–785. doi: 10.1128/jcm.33.3.784-785.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wasilauskas B., Morrell R., Jr Inhibitory effect of the Isolator blood culture system on growth of Mycobacterium avium-M. intracellulare in BACTEC 12B bottles. J Clin Microbiol. 1994 Mar;32(3):654–657. doi: 10.1128/jcm.32.3.654-657.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Whittier P. S., Westfall K., Setterquist S., Hopfer R. L. Evaluation of the Septi-Chek AFB system in the recovery of mycobacteria. Eur J Clin Microbiol Infect Dis. 1992 Oct;11(10):915–918. doi: 10.1007/BF01962373. [DOI] [PubMed] [Google Scholar]