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. 1997 Mar;35(3):596–599. doi: 10.1128/jcm.35.3.596-599.1997

Large-restriction-fragment analysis of Mycobacterium kansasii genomic DNA and its application in molecular typing.

Y Iinuma 1, S Ichiyama 1, Y Hasegawa 1, K Shimokata 1, S Kawahara 1, T Matsushima 1
PMCID: PMC229634  PMID: 9041396

Abstract

Large-restriction-fragment (LRF) polymorphisms in Mycobacterium kansasii isolates from 84 patients with bronchopulmonary infections in Japan between the 1960s and 1995 were studied by pulsed-field gel electrophoresis (PFGE). Chromosomal fragments digested with VspI were most suitable for PFGE separation of 16 to 21 fragments of between 40 and 550 kbp. All 84 isolates and the type strain M. kansasii ATCC 12478 were successfully typed by LRF analysis with VspI digestion. Twenty-one distinctive LRF types were identified, and the LRF patterns tested over time were reproducible and stable. A computer-assisted dendrogram of the percent similarity demonstrated that isolates of 18 LRF types had relatively close genetic relatedness, while isolates of the remaining 3 types showed divergence. Sequence analysis of the 16S rRNA gene in the isolates showing divergent genetic relatedness revealed a sequence identical to that of a previously reported subspecies of M. kansasii. In the Chugoku district of Japan, 11 cases of M. kansasii infection which occurred in workers in a coastal industrial zone between 1982 and 1993 were caused by one particular strain tentatively named LRF type M. When both detailed demographic data for the patients and ecologic data for the M. kansasii isolates are obtained, LRF typing may be of potential use for investigating the source and transmission of M. kansasii infection.

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Selected References

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  1. Arbeit R. D., Slutsky A., Barber T. W., Maslow J. N., Niemczyk S., Falkinham J. O., 3rd, O'Connor G. T., von Reyn C. F. Genetic diversity among strains of Mycobacterium avium causing monoclonal and polyclonal bacteremia in patients with AIDS. J Infect Dis. 1993 Jun;167(6):1384–1390. doi: 10.1093/infdis/167.6.1384. [DOI] [PubMed] [Google Scholar]
  2. Bannerman T. L., Hancock G. A., Tenover F. C., Miller J. M. Pulsed-field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus. J Clin Microbiol. 1995 Mar;33(3):551–555. doi: 10.1128/jcm.33.3.551-555.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burns D. N., Wallace R. J., Jr, Schultz M. E., Zhang Y. S., Zubairi S. Q., Pang Y. J., Gibert C. L., Brown B. A., Noel E. S., Gordin F. M. Nosocomial outbreak of respiratory tract colonization with Mycobacterium fortuitum: demonstration of the usefulness of pulsed-field gel electrophoresis in an epidemiologic investigation. Am Rev Respir Dis. 1991 Nov;144(5):1153–1159. doi: 10.1164/ajrccm/144.5.1153. [DOI] [PubMed] [Google Scholar]
  4. Carpenter J. L., Parks J. M. Mycobacterium kansasii infections in patients positive for human immunodeficiency virus. Rev Infect Dis. 1991 Sep-Oct;13(5):789–796. doi: 10.1093/clinids/13.5.789. [DOI] [PubMed] [Google Scholar]
  5. Engel H. W., Berwald L. G., Grange J. M., Kubin M. Phage typing of Mycobacterium kansasii. Tubercle. 1980 Mar;61(1):11–19. doi: 10.1016/0041-3879(80)90054-9. [DOI] [PubMed] [Google Scholar]
  6. Engel H. W., Berwald L. G., Havelaar A. H. The occurrence of Mycobacterium kansasii in tapwater. Tubercle. 1980 Mar;61(1):21–26. doi: 10.1016/0041-3879(80)90055-0. [DOI] [PubMed] [Google Scholar]
  7. Falkinham J. O., 3rd Epidemiology of infection by nontuberculous mycobacteria. Clin Microbiol Rev. 1996 Apr;9(2):177–215. doi: 10.1128/cmr.9.2.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hermans P. W., van Soolingen D., van Embden J. D. Characterization of a major polymorphic tandem repeat in Mycobacterium tuberculosis and its potential use in the epidemiology of Mycobacterium kansasii and Mycobacterium gordonae. J Bacteriol. 1992 Jun;174(12):4157–4165. doi: 10.1128/jb.174.12.4157-4165.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Huang Z. H., Ross B. C., Dwyer B. Identification of Mycobacterium kansasii by DNA hybridization. J Clin Microbiol. 1991 Oct;29(10):2125–2129. doi: 10.1128/jcm.29.10.2125-2129.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Joynson D. H. Water: the natural habitat of Mycobacterium kansasii? Tubercle. 1979 Jun;60(2):77–81. doi: 10.1016/0041-3879(79)90039-4. [DOI] [PubMed] [Google Scholar]
  11. Kubín M., Svandová E., Medek B., Chobot S., Olsovský Z. Mycobacterium kansasii infection in an endemic area of Czechoslovakia. Tubercle. 1980 Dec;61(4):207–212. doi: 10.1016/0041-3879(80)90040-9. [DOI] [PubMed] [Google Scholar]
  12. Kusunoki S., Ezaki T., Tamesada M., Hatanaka Y., Asano K., Hashimoto Y., Yabuuchi E. Application of colorimetric microdilution plate hybridization for rapid genetic identification of 22 Mycobacterium species. J Clin Microbiol. 1991 Aug;29(8):1596–1603. doi: 10.1128/jcm.29.8.1596-1603.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lillo M., Orengo S., Cernoch P., Harris R. L. Pulmonary and disseminated infection due to Mycobacterium kansasii: a decade of experience. Rev Infect Dis. 1990 Sep-Oct;12(5):760–767. doi: 10.1093/clinids/12.5.760. [DOI] [PubMed] [Google Scholar]
  14. Maloney S., Welbel S., Daves B., Adams K., Becker S., Bland L., Arduino M., Wallace R., Jr, Zhang Y., Buck G. Mycobacterium abscessus pseudoinfection traced to an automated endoscope washer: utility of epidemiologic and laboratory investigation. J Infect Dis. 1994 May;169(5):1166–1169. doi: 10.1093/infdis/169.5.1166. [DOI] [PubMed] [Google Scholar]
  15. Mazurek G. H., Hartman S., Zhang Y., Brown B. A., Hector J. S., Murphy D., Wallace R. J., Jr Large DNA restriction fragment polymorphism in the Mycobacterium avium-M. intracellulare complex: a potential epidemiologic tool. J Clin Microbiol. 1993 Feb;31(2):390–394. doi: 10.1128/jcm.31.2.390-394.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rogall T., Wolters J., Flohr T., Böttger E. C. Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium. Int J Syst Bacteriol. 1990 Oct;40(4):323–330. doi: 10.1099/00207713-40-4-323. [DOI] [PubMed] [Google Scholar]
  17. Ross B. C., Jackson K., Yang M., Sievers A., Dwyer B. Identification of a genetically distinct subspecies of Mycobacterium kansasii. J Clin Microbiol. 1992 Nov;30(11):2930–2933. doi: 10.1128/jcm.30.11.2930-2933.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ross B. C., Raios K., Jackson K., Dwyer B. Molecular cloning of a highly repeated DNA element from Mycobacterium tuberculosis and its use as an epidemiological tool. J Clin Microbiol. 1992 Apr;30(4):942–946. doi: 10.1128/jcm.30.4.942-946.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schaefer W. B. Serologic identification and classification of the atypical mycobacteria by their agglutination. Am Rev Respir Dis. 1965 Dec;92(6):85–93. doi: 10.1164/arrd.1965.92.6P2.85. [DOI] [PubMed] [Google Scholar]
  20. Schulze-Röbbecke R., Janning B., Fischeder R. Occurrence of mycobacteria in biofilm samples. Tuber Lung Dis. 1992 Jun;73(3):141–144. doi: 10.1016/0962-8479(92)90147-C. [DOI] [PubMed] [Google Scholar]
  21. Slutsky A. M., Arbeit R. D., Barber T. W., Rich J., von Reyn C. F., Pieciak W., Barlow M. A., Maslow J. N. Polyclonal infections due to Mycobacterium avium complex in patients with AIDS detected by pulsed-field gel electrophoresis of sequential clinical isolates. J Clin Microbiol. 1994 Jul;32(7):1773–1778. doi: 10.1128/jcm.32.7.1773-1778.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Steadham J. E. High-catalase strains of Mycobacterium kansasii isolated from water in Texas. J Clin Microbiol. 1980 May;11(5):496–498. doi: 10.1128/jcm.11.5.496-498.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tsukamura M. A review of the methods of identification and differentiation of mycobacteria. Rev Infect Dis. 1981 Sep-Oct;3(5):841–861. doi: 10.1093/clinids/3.5.841. [DOI] [PubMed] [Google Scholar]
  24. Tsukamura M., Kita N., Shimoide H., Arakawa H., Kuze A. Studies on the epidemiology of nontuberculous mycobacteriosis in Japan. Am Rev Respir Dis. 1988 Jun;137(6):1280–1284. doi: 10.1164/ajrccm/137.6.1280. [DOI] [PubMed] [Google Scholar]
  25. Wolinsky E. Nontuberculous mycobacteria and associated diseases. Am Rev Respir Dis. 1979 Jan;119(1):107–159. doi: 10.1164/arrd.1979.119.1.107. [DOI] [PubMed] [Google Scholar]
  26. Yang M., Ross B. C., Dwyer B. Identification of an insertion sequence-like element in a subspecies of Mycobacterium kansasii. J Clin Microbiol. 1993 Aug;31(8):2074–2079. doi: 10.1128/jcm.31.8.2074-2079.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Zhang Y., Mazurek G. H., Cave M. D., Eisenach K. D., Pang Y., Murphy D. T., Wallace R. J., Jr DNA polymorphisms in strains of Mycobacterium tuberculosis analyzed by pulsed-field gel electrophoresis: a tool for epidemiology. J Clin Microbiol. 1992 Jun;30(6):1551–1556. doi: 10.1128/jcm.30.6.1551-1556.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. van Soolingen D., Hermans P. W., de Haas P. E., Soll D. R., van Embden J. D. Occurrence and stability of insertion sequences in Mycobacterium tuberculosis complex strains: evaluation of an insertion sequence-dependent DNA polymorphism as a tool in the epidemiology of tuberculosis. J Clin Microbiol. 1991 Nov;29(11):2578–2586. doi: 10.1128/jcm.29.11.2578-2586.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]

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