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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1997 Mar;35(3):741–744. doi: 10.1128/jcm.35.3.741-744.1997

Minimal effect of delayed sample processing on results of quantitative PCR for cytomegalovirus DNA in leukocytes compared to results of an antigenemia assay.

P Schäfer 1, W Tenschert 1, K Gutensohn 1, R Laufs 1
PMCID: PMC229663  PMID: 9041425

Abstract

Quantitative cytomegalovirus antigenemia and DNAemia were determined in peripheral leukocytes of 25 patients stored for up to 72 h at room temperature (RT) and 4 degrees C before processing. Numbers of antigen-positive cells significantly decreased with time. The decline was greater at RT than at 4 degrees C. In contrast, no significant alterations in DNAemia occurred.

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Selected References

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  1. Boeckh M., Bowden R. A., Goodrich J. M., Pettinger M., Meyers J. D. Cytomegalovirus antigen detection in peripheral blood leukocytes after allogeneic marrow transplantation. Blood. 1992 Sep 1;80(5):1358–1364. [PubMed] [Google Scholar]
  2. Boeckh M., Woogerd P. M., Stevens-Ayers T., Ray C. G., Bowden R. A. Factors influencing detection of quantitative cytomegalovirus antigenemia. J Clin Microbiol. 1994 Mar;32(3):832–834. doi: 10.1128/jcm.32.3.832-834.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Drouet E., Colimon R., Michelson S., Fourcade N., Niveleau A., Ducerf C., Boibieux A., Chevallier M., Denoyel G. Monitoring levels of human cytomegalovirus DNA in blood after liver transplantation. J Clin Microbiol. 1995 Feb;33(2):389–394. doi: 10.1128/jcm.33.2.389-394.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gass P., Kiessling M., Schäfer P., Mester C., Schmitt H. P., Kühn J. E. Detection of human cytomegalovirus DNA in paraffin sections of human brain by polymerase chain reaction and the occurrence of false negative results. J Neurol Neurosurg Psychiatry. 1993 Feb;56(2):211–214. doi: 10.1136/jnnp.56.2.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gerdes J. C., Spees E. K., Fitting K., Hiraki J., Sheehan M., Duda D., Jarvi T., Roehl C., Robertson A. D. Prospective study utilizing a quantitative polymerase chain reaction for detection of cytomegalovirus DNA in the blood of renal transplant patients. Transplant Proc. 1993 Feb;25(1 Pt 2):1411–1413. [PubMed] [Google Scholar]
  6. Gerna G., Furione M., Baldanti F., Sarasini A. Comparative quantitation of human cytomegalovirus DNA in blood leukocytes and plasma of transplant and AIDS patients. J Clin Microbiol. 1994 Nov;32(11):2709–2717. doi: 10.1128/jcm.32.11.2709-2717.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gerna G., Revello M. G., Percivalle E., Morini F. Comparison of different immunostaining techniques and monoclonal antibodies to the lower matrix phosphoprotein (pp65) for optimal quantitation of human cytomegalovirus antigenemia. J Clin Microbiol. 1992 May;30(5):1232–1237. doi: 10.1128/jcm.30.5.1232-1237.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gerna G., Zipeto D., Parea M., Revello M. G., Silini E., Percivalle E., Zavattoni M., Grossi P., Milanesi G. Monitoring of human cytomegalovirus infections and ganciclovir treatment in heart transplant recipients by determination of viremia, antigenemia, and DNAemia. J Infect Dis. 1991 Sep;164(3):488–498. doi: 10.1093/infdis/164.3.488. [DOI] [PubMed] [Google Scholar]
  9. Koskinen P. K., Nieminen M. S., Mattila S. P., Häyry P. J., Lautenschlager I. T. The correlation between symptomatic CMV infection and CMV antigenemia in heart allograft recipients. Transplantation. 1993 Mar;55(3):547–551. doi: 10.1097/00007890-199303000-00017. [DOI] [PubMed] [Google Scholar]
  10. Kühn J. E., Wendland T., Schäfer P., Möhring K., Wieland U., Elgas M., Eggers H. J. Monitoring of renal allograft recipients by quantitation of human cytomegalovirus genomes in peripheral blood leukocytes. J Med Virol. 1994 Dec;44(4):398–405. doi: 10.1002/jmv.1890440416. [DOI] [PubMed] [Google Scholar]
  11. Landry M. L., Ferguson D., Cohen S., Huber K., Wetherill P. Effect of delayed specimen processing on cytomegalovirus antigenemia test results. J Clin Microbiol. 1995 Jan;33(1):257–259. doi: 10.1128/jcm.33.1.257-259.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Miller H., Rossier E., Milk R., Thomas C. Prospective study of cytomegalovirus antigenemia in allograft recipients. J Clin Microbiol. 1991 May;29(5):1054–1055. doi: 10.1128/jcm.29.5.1054-1055.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Niubò J., Pérez J. L., Carvajal A., Ardanuy C., Martín R. Effect of delayed processing of blood samples on performance of cytomegalovirus antigenemia assay. J Clin Microbiol. 1994 Apr;32(4):1119–1120. doi: 10.1128/jcm.32.4.1119-1120.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Saltzman R. L., Quirk M. R., Jordan M. C. High levels of circulating cytomegalovirus DNA reflect visceral organ disease in viremic immunosuppressed patients other than marrow recipients. J Clin Invest. 1992 Nov;90(5):1832–1838. doi: 10.1172/JCI116059. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schäfer P., Braun R. W., Möhring K., Henco K., Kang J., Wendland T., Kühn J. E. Quantitative determination of human cytomegalovirus target sequences in peripheral blood leukocytes by nested polymerase chain reaction and temperature gradient gel electrophoresis. J Gen Virol. 1993 Dec;74(Pt 12):2699–2707. doi: 10.1099/0022-1317-74-12-2699. [DOI] [PubMed] [Google Scholar]
  16. The T. H., van den Berg A. P., Harmsen M. C., van der Bij W., van Son W. J. The cytomegalovirus antigenemia assay: a plea for standardization. Scand J Infect Dis Suppl. 1995;99:25–29. [PubMed] [Google Scholar]
  17. The T. H., van der Bij W., van den Berg A. P., van der Giessen M., Weits J., Sprenger H. G., van Son W. J. Cytomegalovirus antigenemia. Rev Infect Dis. 1990 Sep-Oct;12 (Suppl 7):S734–S744. [PubMed] [Google Scholar]
  18. The T. H., van der Ploeg M., van den Berg A. P., Vlieger A. M., van der Giessen M., van Son W. J. Direct detection of cytomegalovirus in peripheral blood leukocytes--a review of the antigenemia assay and polymerase chain reaction. Transplantation. 1992 Aug;54(2):193–198. doi: 10.1097/00007890-199208000-00001. [DOI] [PubMed] [Google Scholar]
  19. Zipeto D., Baldanti F., Zella D., Furione M., Cavicchini A., Milanesi G., Gerna G. Quantification of human cytomegalovirus DNA in peripheral blood polymorphonuclear leukocytes of immunocompromised patients by the polymerase chain reaction. J Virol Methods. 1993 Sep;44(1):45–55. doi: 10.1016/0166-0934(93)90006-d. [DOI] [PubMed] [Google Scholar]
  20. Zipeto D., Morris S., Hong C., Dowling A., Wolitz R., Merigan T. C., Rasmussen L. Human cytomegalovirus (CMV) DNA in plasma reflects quantity of CMV DNA present in leukocytes. J Clin Microbiol. 1995 Oct;33(10):2607–2611. doi: 10.1128/jcm.33.10.2607-2611.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. van den Berg A. P., Klompmaker I. J., Haagsma E. B., Scholten-Sampson A., Bijleveld C. M., Schirm J., van der Giessen M., Slooff M. J., The T. H. Antigenemia in the diagnosis and monitoring of active cytomegalovirus infection after liver transplantation. J Infect Dis. 1991 Aug;164(2):265–270. doi: 10.1093/infdis/164.2.265. [DOI] [PubMed] [Google Scholar]
  22. van den Berg A. P., van der Bij W., van Son W. J., Anema J., van der Giessen M., Schirm J., Tegzess A. M., The T. H. Cytomegalovirus antigenemia as a useful marker of symptomatic cytomegalovirus infection after renal transplantation--a report of 130 consecutive patients. Transplantation. 1989 Dec;48(6):991–995. doi: 10.1097/00007890-198912000-00019. [DOI] [PubMed] [Google Scholar]
  23. van der Bij W., Schirm J., Torensma R., van Son W. J., Tegzess A. M., The T. H. Comparison between viremia and antigenemia for detection of cytomegalovirus in blood. J Clin Microbiol. 1988 Dec;26(12):2531–2535. doi: 10.1128/jcm.26.12.2531-2535.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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