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. 1997 Mar;35(3):759–763. doi: 10.1128/jcm.35.3.759-763.1997

Specific identification of the enteropathogens Campylobacter jejuni and Campylobacter coli by using a PCR test based on the ceuE gene encoding a putative virulence determinant.

I Gonzalez 1, K A Grant 1, P T Richardson 1, S F Park 1, M D Collins 1
PMCID: PMC229667  PMID: 9041429

Abstract

A PCR method for the rapid identification and discrimination of thermophilic Campylobacter jejuni and Campylobacter coli was developed by using a gene encoding a protein involved in siderophore transport (ceuE). A nucleotide sequence divergence of approximately 13% in the ceuE genes of C. jejuni and C. coli facilitated the design of two species-specific PCR primer sets. The specificity of the PCR amplification reactions was confirmed by using two nonradioactively labelled species-specific internal oligonucleotide hybridization probes for each of these species.

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Selected References

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  1. Bustamante V. H., Puente J. L., Sánchez-López F., Bobadilla M., Calva E. Identification of Campylobacter jejuni and C. coli using the rpoB gene and a cryptic DNA fragment from C. jejuni. Gene. 1995 Nov 7;165(1):1–8. doi: 10.1016/0378-1119(95)00515-8. [DOI] [PubMed] [Google Scholar]
  2. Cardarelli-Leite P., Blom K., Patton C. M., Nicholson M. A., Steigerwalt A. G., Hunter S. B., Brenner D. J., Barrett T. J., Swaminathan B. Rapid identification of Campylobacter species by restriction fragment length polymorphism analysis of a PCR-amplified fragment of the gene coding for 16S rRNA. J Clin Microbiol. 1996 Jan;34(1):62–67. doi: 10.1128/jcm.34.1.62-67.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Docherty L., Adams M. R., Patel P., McFadden J. The magnetic immuno-polymerase chain reaction assay for the detection of Campylobacter in milk and poultry. Lett Appl Microbiol. 1996 Apr;22(4):288–292. doi: 10.1111/j.1472-765x.1996.tb01163.x. [DOI] [PubMed] [Google Scholar]
  4. Eyers M., Chapelle S., Van Camp G., Goossens H., De Wachter R. Discrimination among thermophilic Campylobacter species by polymerase chain reaction amplification of 23S rRNA gene fragments. J Clin Microbiol. 1993 Dec;31(12):3340–3343. doi: 10.1128/jcm.31.12.3340-3343.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Giesendorf B. A., van Belkum A., Koeken A., Stegeman H., Henkens M. H., van der Plas J., Goossens H., Niesters H. G., Quint W. G. Development of species-specific DNA probes for Campylobacter jejuni, Campylobacter coli, and Campylobacter lari by polymerase chain reaction fingerprinting. J Clin Microbiol. 1993 Jun;31(6):1541–1546. doi: 10.1128/jcm.31.6.1541-1546.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Griffiths P. L., Moreno G. S., Park R. W. Differentiation between thermophilic Campylobacter species by species-specific antibodies. J Appl Bacteriol. 1992 Jun;72(6):467–474. doi: 10.1111/j.1365-2672.1992.tb01860.x. [DOI] [PubMed] [Google Scholar]
  7. Kirk R., Rowe M. T. A PCR assay for the detection of Campylobacter jejuni and Campylobacter coli in water. Lett Appl Microbiol. 1994 Nov;19(5):301–303. doi: 10.1111/j.1472-765x.1994.tb00460.x. [DOI] [PubMed] [Google Scholar]
  8. Korolik V., Coloe P. J., Krishnapillai V. A specific DNA probe for the identification of Campylobacter jejuni. J Gen Microbiol. 1988 Feb;134(2):521–529. doi: 10.1099/00221287-134-2-521. [DOI] [PubMed] [Google Scholar]
  9. Oyofo B. A., Thornton S. A., Burr D. H., Trust T. J., Pavlovskis O. R., Guerry P. Specific detection of Campylobacter jejuni and Campylobacter coli by using polymerase chain reaction. J Clin Microbiol. 1992 Oct;30(10):2613–2619. doi: 10.1128/jcm.30.10.2613-2619.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Park S. F., Richardson P. T. Molecular characterization of a Campylobacter jejuni lipoprotein with homology to periplasmic siderophore-binding proteins. J Bacteriol. 1995 May;177(9):2259–2264. doi: 10.1128/jb.177.9.2259-2264.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Patton C. M., Wachsmuth I. K., Evins G. M., Kiehlbauch J. A., Plikaytis B. D., Troup N., Tompkins L., Lior H. Evaluation of 10 methods to distinguish epidemic-associated Campylobacter strains. J Clin Microbiol. 1991 Apr;29(4):680–688. doi: 10.1128/jcm.29.4.680-688.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Penner J. L. The genus Campylobacter: a decade of progress. Clin Microbiol Rev. 1988 Apr;1(2):157–172. doi: 10.1128/cmr.1.2.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Richardson P. T., Park S. F. Enterochelin acquisition in Campylobacter coli: characterization of components of a binding-protein-dependent transport system. Microbiology. 1995 Dec;141(Pt 12):3181–3191. doi: 10.1099/13500872-141-12-3181. [DOI] [PubMed] [Google Scholar]
  14. Stucki U., Frey J., Nicolet J., Burnens A. P. Identification of Campylobacter jejuni on the basis of a species-specific gene that encodes a membrane protein. J Clin Microbiol. 1995 Apr;33(4):855–859. doi: 10.1128/jcm.33.4.855-859.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tenover F. C., Carlson L., Barbagallo S., Nachamkin I. DNA probe culture confirmation assay for identification of thermophilic Campylobacter species. J Clin Microbiol. 1990 Jun;28(6):1284–1287. doi: 10.1128/jcm.28.6.1284-1287.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Totten P. A., Patton C. M., Tenover F. C., Barrett T. J., Stamm W. E., Steigerwalt A. G., Lin J. Y., Holmes K. K., Brenner D. J. Prevalence and characterization of hippurate-negative Campylobacter jejuni in King County, Washington. J Clin Microbiol. 1987 Sep;25(9):1747–1752. doi: 10.1128/jcm.25.9.1747-1752.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wang Y., Taylor D. E. Natural transformation in Campylobacter species. J Bacteriol. 1990 Feb;172(2):949–955. doi: 10.1128/jb.172.2.949-955.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wegmüller B., Lüthy J., Candrian U. Direct polymerase chain reaction detection of Campylobacter jejuni and Campylobacter coli in raw milk and dairy products. Appl Environ Microbiol. 1993 Jul;59(7):2161–2165. doi: 10.1128/aem.59.7.2161-2165.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

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