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. 1997 Mar;35(3):788–790. doi: 10.1128/jcm.35.3.788-790.1997

Evaluation of a quantitative plasma PCR plate assay for detecting cytomegalovirus infection in marrow transplant recipients.

G M Gallez-Hawkins 1, B R Tegtmeier 1, A ter Veer 1, J C Niland 1, S J Forman 1, J A Zaia 1
PMCID: PMC229676  PMID: 9041438

Abstract

A plasma PCR test, using a nonradioactive PCR plate assay, was evaluated for detection of human cytomegalovirus reactivation. This assay was compared to Southern blotting and found to perform well. As a noncompetitive method of quantitation, it was similar to a competitive method for detecting the number of genome copies per milliliter of plasma in marrow transplant recipients. This is a technically simplified assay with potential for adaptation to automation.

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Selected References

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