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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1997 Mar;35(3):799–801. doi: 10.1128/jcm.35.3.799-801.1997

Specificity of IS6110-based amplification assays for Mycobacterium tuberculosis complex.

S H Gillespie, T D McHugh, L E Newport
PMCID: PMC229679  PMID: 9041441

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Selected References

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  1. Chaves F., Yang Z., el Hajj H., Alonso M., Burman W. J., Eisenach K. D., Dronda F., Bates J. H., Cave M. D. Usefulness of the secondary probe pTBN12 in DNA fingerprinting of Mycobacterium tuberculosis. J Clin Microbiol. 1996 May;34(5):1118–1123. doi: 10.1128/jcm.34.5.1118-1123.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dalovisio J. R., Montenegro-James S., Kemmerly S. A., Genre C. F., Chambers R., Greer D., Pankey G. A., Failla D. M., Haydel K. G., Hutchinson L. Comparison of the amplified Mycobacterium tuberculosis (MTB) direct test, Amplicor MTB PCR, and IS6110-PCR for detection of MTB in respiratory specimens. Clin Infect Dis. 1996 Nov;23(5):1099–1108. doi: 10.1093/clinids/23.5.1099. [DOI] [PubMed] [Google Scholar]
  3. Dalovisio J. R., Montenegro-James S., Kemmerly S. A., Genre C. F., Chambers R., Greer D., Pankey G. A., Failla D. M., Haydel K. G., Hutchinson L. Comparison of the amplified Mycobacterium tuberculosis (MTB) direct test, Amplicor MTB PCR, and IS6110-PCR for detection of MTB in respiratory specimens. Clin Infect Dis. 1996 Nov;23(5):1099–1108. doi: 10.1093/clinids/23.5.1099. [DOI] [PubMed] [Google Scholar]
  4. Desjardin L. E., Perkins M. D., Teixeira L., Cave M. D., Eisenach K. D. Alkaline decontamination of sputum specimens adversely affects stability of mycobacterial mRNA. J Clin Microbiol. 1996 Oct;34(10):2435–2439. doi: 10.1128/jcm.34.10.2435-2439.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doucet-Populaire F., Lalande V., Carpentier E., Bourgoin A., Dailloux M., Bollet C., Vachée A., Moinard D., Texier-Maugein J., Carbonnelle B. A blind study of the polymerase chain reaction for the detection of Mycobacterium tuberculosis DNA. Azay Mycobacteria Study Group. Tuber Lung Dis. 1996 Aug;77(4):358–362. doi: 10.1016/s0962-8479(96)90102-1. [DOI] [PubMed] [Google Scholar]
  6. Eisenach K. D., Cave M. D., Bates J. H., Crawford J. T. Polymerase chain reaction amplification of a repetitive DNA sequence specific for Mycobacterium tuberculosis. J Infect Dis. 1990 May;161(5):977–981. doi: 10.1093/infdis/161.5.977. [DOI] [PubMed] [Google Scholar]
  7. Eisenach K. D., Sifford M. D., Cave M. D., Bates J. H., Crawford J. T. Detection of Mycobacterium tuberculosis in sputum samples using a polymerase chain reaction. Am Rev Respir Dis. 1991 Nov;144(5):1160–1163. doi: 10.1164/ajrccm/144.5.1160. [DOI] [PubMed] [Google Scholar]
  8. Eisenach K. D., Sifford M. D., Cave M. D., Bates J. H., Crawford J. T. Detection of Mycobacterium tuberculosis in sputum samples using a polymerase chain reaction. Am Rev Respir Dis. 1991 Nov;144(5):1160–1163. doi: 10.1164/ajrccm/144.5.1160. [DOI] [PubMed] [Google Scholar]
  9. Fischl M. A., Uttamchandani R. B., Daikos G. L., Poblete R. B., Moreno J. N., Reyes R. R., Boota A. M., Thompson L. M., Cleary T. J., Lai S. An outbreak of tuberculosis caused by multiple-drug-resistant tubercle bacilli among patients with HIV infection. Ann Intern Med. 1992 Aug 1;117(3):177–183. doi: 10.7326/0003-4819-117-3-177. [DOI] [PubMed] [Google Scholar]
  10. Hellyer T. J., DesJardin L. E., Assaf M. K., Bates J. H., Cave M. D., Eisenach K. D. Specificity of IS6110-based amplification assays for Mycobacterium tuberculosis complex. J Clin Microbiol. 1996 Nov;34(11):2843–2846. doi: 10.1128/jcm.34.11.2843-2846.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hellyer T. J., DesJardin L. E., Assaf M. K., Bates J. H., Cave M. D., Eisenach K. D. Specificity of IS6110-based amplification assays for Mycobacterium tuberculosis complex. J Clin Microbiol. 1996 Nov;34(11):2843–2846. doi: 10.1128/jcm.34.11.2843-2846.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hellyer T. J., Fletcher T. W., Bates J. H., Stead W. W., Templeton G. L., Cave M. D., Eisenach K. D. Strand displacement amplification and the polymerase chain reaction for monitoring response to treatment in patients with pulmonary tuberculosis. J Infect Dis. 1996 Apr;173(4):934–941. doi: 10.1093/infdis/173.4.934. [DOI] [PubMed] [Google Scholar]
  13. Kent L., McHugh T. D., Billington O., Dale J. W., Gillespie S. H. Demonstration of homology between IS6110 of Mycobacterium tuberculosis and DNAs of other Mycobacterium spp.? J Clin Microbiol. 1995 Sep;33(9):2290–2293. doi: 10.1128/jcm.33.9.2290-2293.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McAdam R. A., Hermans P. W., van Soolingen D., Zainuddin Z. F., Catty D., van Embden J. D., Dale J. W. Characterization of a Mycobacterium tuberculosis insertion sequence belonging to the IS3 family. Mol Microbiol. 1990 Sep;4(9):1607–1613. doi: 10.1111/j.1365-2958.1990.tb02073.x. [DOI] [PubMed] [Google Scholar]
  15. Noordhoek G. T., van Embden J. D., Kolk A. H. Reliability of nucleic acid amplification for detection of Mycobacterium tuberculosis: an international collaborative quality control study among 30 laboratories. J Clin Microbiol. 1996 Oct;34(10):2522–2525. doi: 10.1128/jcm.34.10.2522-2525.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Noordhoek G. T., van Embden J. D., Kolk A. H. Reliability of nucleic acid amplification for detection of Mycobacterium tuberculosis: an international collaborative quality control study among 30 laboratories. J Clin Microbiol. 1996 Oct;34(10):2522–2525. doi: 10.1128/jcm.34.10.2522-2525.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pietrzak J., Frei R., Senn H. P., Moroni C. Comparison of polymerase chain reaction with standard methods in the diagnosis of Mycobacterium tuberculosis infection. Eur J Clin Microbiol Infect Dis. 1994 Dec;13(12):1079–1083. doi: 10.1007/BF02111834. [DOI] [PubMed] [Google Scholar]
  18. Pietrzak J., Frei R., Senn H. P., Moroni C. Comparison of polymerase chain reaction with standard methods in the diagnosis of Mycobacterium tuberculosis infection. Eur J Clin Microbiol Infect Dis. 1994 Dec;13(12):1079–1083. doi: 10.1007/BF02111834. [DOI] [PubMed] [Google Scholar]
  19. Querol J. M., Farga M. A., Granda D., Gimeno C., García-de-Lomas J. The utility of polymerase chain reaction (PCR) in the diagnosis of pulmonary tuberculosis. Chest. 1995 Jun;107(6):1631–1635. doi: 10.1378/chest.107.6.1631. [DOI] [PubMed] [Google Scholar]
  20. Querol J. M., Farga M. A., Granda D., Gimeno C., García-de-Lomas J. The utility of polymerase chain reaction (PCR) in the diagnosis of pulmonary tuberculosis. Chest. 1995 Jun;107(6):1631–1635. doi: 10.1378/chest.107.6.1631. [DOI] [PubMed] [Google Scholar]
  21. Rish J. A., Eisenach K. D., Cave M. D., Reddy M. V., Gangadharam P. R., Bates J. H. Polymerase chain reaction detection of Mycobacterium tuberculosis in formalin-fixed tissue. Am J Respir Crit Care Med. 1996 Apr;153(4 Pt 1):1419–1423. doi: 10.1164/ajrccm.153.4.8616575. [DOI] [PubMed] [Google Scholar]
  22. Rish J. A., Eisenach K. D., Cave M. D., Reddy M. V., Gangadharam P. R., Bates J. H. Polymerase chain reaction detection of Mycobacterium tuberculosis in formalin-fixed tissue. Am J Respir Crit Care Med. 1996 Apr;153(4 Pt 1):1419–1423. doi: 10.1164/ajrccm.153.4.8616575. [DOI] [PubMed] [Google Scholar]
  23. Walker G. T., Little M. C., Nadeau J. G., Shank D. D. Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system. Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):392–396. doi: 10.1073/pnas.89.1.392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wilson S. M., McNerney R., Nye P. M., Godfrey-Faussett P. D., Stoker N. G., Voller A. Progress toward a simplified polymerase chain reaction and its application to diagnosis of tuberculosis. J Clin Microbiol. 1993 Apr;31(4):776–782. doi: 10.1128/jcm.31.4.776-782.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

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