Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1997 Apr;35(4):877–880. doi: 10.1128/jcm.35.4.877-880.1997

Evaluation of latex reagents for rapid identification of Candida albicans and C. krusei colonies.

A M Freydiere 1, L Buchaille 1, R Guinet 1, Y Gille 1
PMCID: PMC229694  PMID: 9157146

Abstract

A total of 322 yeast strains and yeastlike organisms belonging to the genera Candida, Cryptococcus, Geotrichum, Saccharomyces, and Trichosporon were tested with the new monoclonal antibody-based Bichro-latex albicans and Krusei color latex tests. Comparison of results with those obtained by conventional identification methods showed 100% sensitivity for both latex tests and 100% and 95% specificity for the Bichro-latex albicans and Krusei color tests, respectively. Because the test is easy to read and quick to perform, the Bichro-latex albicans test may be useful for rapid identification of Candida albicans colonies in the clinical laboratory.

Full Text

The Full Text of this article is available as a PDF (64.9 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baumgartner C., Freydiere A. M., Gille Y. Direct identification and recognition of yeast species from clinical material by using albicans ID and CHROMagar Candida plates. J Clin Microbiol. 1996 Feb;34(2):454–456. doi: 10.1128/jcm.34.2.454-456.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beighton D., Ludford R., Clark D. T., Brailsford S. R., Pankhurst C. L., Tinsley G. F., Fiske J., Lewis D., Daly B., Khalifa N. Use of CHROMagar Candida medium for isolation of yeasts from dental samples. J Clin Microbiol. 1995 Nov;33(11):3025–3027. doi: 10.1128/jcm.33.11.3025-3027.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brawner D. L., Cutler J. E. Variability in expression of a cell surface determinant on Candida albicans as evidenced by an agglutinating monoclonal antibody. Infect Immun. 1984 Mar;43(3):966–972. doi: 10.1128/iai.43.3.966-972.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bruneau S. M., Guinet R. M. Characterization of Candida antigens by crossed-immunoaffinoelectrophoresis. Sabouraudia. 1985 Apr;23(2):107–118. [PubMed] [Google Scholar]
  5. Bruneau S., Guinet R. Rapid identification of medically important yeasts by electrophoretic protein patterns. FEMS Microbiol Lett. 1989 Apr;49(2-3):329–333. doi: 10.1016/0378-1097(89)90062-1. [DOI] [PubMed] [Google Scholar]
  6. Chaffin W. L., Skudlarek J., Morrow K. J. Variable expression of a surface determinant during proliferation of Candida albicans. Infect Immun. 1988 Feb;56(2):302–309. doi: 10.1128/iai.56.2.302-309.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. De Champs C., Lebeau B., Ambroise-Thomas P., Grillot R. Evaluation of Albicans ID plates. J Clin Microbiol. 1995 Aug;33(8):2227–2228. doi: 10.1128/jcm.33.8.2227-2228.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dealler S. F. Candida albicans colony identification in 5 minutes in a general microbiology laboratory. J Clin Microbiol. 1991 May;29(5):1081–1082. doi: 10.1128/jcm.29.5.1081-1082.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fenn J. P., Segal H., Blevins L., Fawson S., Newcomb-Gayman P., Carroll K. C. Comparison of the Murex Candida albicans CA50 test with germ tube production for identification of C. albicans. Diagn Microbiol Infect Dis. 1996 Jan;24(1):31–35. doi: 10.1016/0732-8893(95)00199-9. [DOI] [PubMed] [Google Scholar]
  10. Freydiere A. M. Evaluation of CHROMagar Candida plates. J Clin Microbiol. 1996 Aug;34(8):2048–2048. doi: 10.1128/jcm.34.8.2048-2048.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fricker H., Monget D., Lebeau B., Babolat M., Ambroise-Thomas P., Grillot R. Identification rapide de Candida albicans: évaluation de "Rapidec albicans". Etude de 444 souches de levures. Ann Biol Clin (Paris) 1992;50(2):103–106. [PubMed] [Google Scholar]
  12. Guinet R. M., Gabriel S. M. Candida albicans group A-specific soluble antigens demonstrated by quantitative immunoelectrophoresis. Infect Immun. 1980 Sep;29(3):853–858. doi: 10.1128/iai.29.3.853-858.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Guinet R., Bruneau S., Marlier H. Rapid identification of Candida albicans by dot-enzyme immunoassay. J Immunoassay. 1991;12(2):225–231. doi: 10.1080/01971529108055068. [DOI] [PubMed] [Google Scholar]
  14. Guinet R., Chanas J., Goullier A., Bonnefoy G., Ambroise-Thomas P. Fatal septicemia due to amphotericin B-resistant Candida lusitaniae. J Clin Microbiol. 1983 Aug;18(2):443–444. doi: 10.1128/jcm.18.2.443-444.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Guinet R., Nerson D., de Closets F., Dupouy-Camet J., Kures L., Marjollet M., Poirot J. L., Ros A., Texier-Maugein J., Volle P. J. Collaborative evaluation in seven laboratories of a standardized micromethod for yeast susceptibility testing. J Clin Microbiol. 1988 Nov;26(11):2307–2312. doi: 10.1128/jcm.26.11.2307-2312.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hopwood V., Poulain D., Fortier B., Evans G., Vernes A. A monoclonal antibody to a cell wall component of Candida albicans. Infect Immun. 1986 Oct;54(1):222–227. doi: 10.1128/iai.54.1.222-227.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lipperheide V., Andraka L., Pontón J., Quindós G. Evaluation of the albicans IDR plate method for the rapid identification of Candida albicans. Mycoses. 1993 Nov-Dec;36(11-12):417–420. doi: 10.1111/j.1439-0507.1993.tb00731.x. [DOI] [PubMed] [Google Scholar]
  18. Mackenzie D. W. Serum tube identification of Candida albicans. J Clin Pathol. 1962 Nov;15(6):563–565. doi: 10.1136/jcp.15.6.563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Miyakawa Y., Kagaya K., Fukazawa Y., Soe G. Production and characterization of agglutinating monoclonal antibodies against predominant antigenic factors for Candida albicans. J Clin Microbiol. 1986 May;23(5):881–886. doi: 10.1128/jcm.23.5.881-886.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Odds F. C., Bernaerts R. CHROMagar Candida, a new differential isolation medium for presumptive identification of clinically important Candida species. J Clin Microbiol. 1994 Aug;32(8):1923–1929. doi: 10.1128/jcm.32.8.1923-1929.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Odds F. C. Resistance of yeasts to azole-derivative antifungals. J Antimicrob Chemother. 1993 Apr;31(4):463–471. doi: 10.1093/jac/31.4.463. [DOI] [PubMed] [Google Scholar]
  22. Perry J. L., Miller G. R., Carr D. L. Rapid, colorimetric identification of Candida albicans. J Clin Microbiol. 1990 Mar;28(3):614–615. doi: 10.1128/jcm.28.3.614-615.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pfaller M. A., Houston A., Coffmann S. Application of CHROMagar Candida for rapid screening of clinical specimens for Candida albicans, Candida tropicalis, Candida krusei, and Candida (Torulopsis) glabrata. J Clin Microbiol. 1996 Jan;34(1):58–61. doi: 10.1128/jcm.34.1.58-61.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Polonelli L., Morace G. Specific and common antigenic determinants of Candida albicans isolates detected by monoclonal antibody. J Clin Microbiol. 1986 Feb;23(2):366–368. doi: 10.1128/jcm.23.2.366-368.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rousselle P., Freydiere A. M., Couillerot P. J., de Montclos H., Gille Y. Rapid identification of Candida albicans by using Albicans ID and fluoroplate agar plates. J Clin Microbiol. 1994 Dec;32(12):3034–3036. doi: 10.1128/jcm.32.12.3034-3036.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. San-Millán R., Ribacoba L., Pontón J., Quindós G. Evaluation of a commercial medium for identification of Candida species. Eur J Clin Microbiol Infect Dis. 1996 Feb;15(2):153–158. doi: 10.1007/BF01591489. [DOI] [PubMed] [Google Scholar]
  27. Schuffenecker I., Freydière A., de Montclos H., Gille Y. Evaluation of four commercial systems for identification of medically important yeasts. Eur J Clin Microbiol Infect Dis. 1993 Apr;12(4):255–260. doi: 10.1007/BF01967255. [DOI] [PubMed] [Google Scholar]
  28. Shinoda T., Kaufman L., Padhye A. A. Comparative evaluation of the Iatron serological Candida check kit and the API 20C kit for identification of medically important Candida species. J Clin Microbiol. 1981 Mar;13(3):513–518. doi: 10.1128/jcm.13.3.513-518.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Strockbine N. A., Largen M. T., Buckley H. R. Production and characterization of three monoclonal antibodies to Candida albicans proteins. Infect Immun. 1984 Mar;43(3):1012–1018. doi: 10.1128/iai.43.3.1012-1018.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. TASCHDJIAN C. L., BURCHALL J. J., KOZINN P. J. Rapid identification of Candida albicans by filamentation on serum and serum substitutes. AMA J Dis Child. 1960 Feb;99:212–215. doi: 10.1001/archpedi.1960.02070030214011. [DOI] [PubMed] [Google Scholar]
  31. Taguchi M., Tsukiji M., Tsuchiya T. Rapid identification of yeasts by serological methods: a combined serological and biological method. Sabouraudia. 1979 Sep;17(3):185–191. doi: 10.1080/00362177985380271. [DOI] [PubMed] [Google Scholar]
  32. Wingard J. R., Merz W. G., Rinaldi M. G., Johnson T. R., Karp J. E., Saral R. Increase in Candida krusei infection among patients with bone marrow transplantation and neutropenia treated prophylactically with fluconazole. N Engl J Med. 1991 Oct 31;325(18):1274–1277. doi: 10.1056/NEJM199110313251803. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES