Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1997 Apr;35(4):965–968. doi: 10.1128/jcm.35.4.965-968.1997

Genetic identification of Mycobacterium bovis BCG by restriction fragment length polymorphism analysis of the direct-repeat region.

S T Howard 1, A Laszlo 1, W M Johnson 1
PMCID: PMC229711  PMID: 9157163

Abstract

Restriction fragment length polymorphism (RFLP) analysis was performed on the direct repeat (DR) regions of 14 strains of Mycobacterium bovis BCG. With AluI-digested DNA, BCG Japanese, Russian, and Mexican had differing RFLP patterns but 11 strains, including Pasteur, Glaxo, and Tice, had an identical pattern not detected in over 60 strains of the M. tuberculosis complex. DR analysis can aid in confirming the identification of clinical BCG isolates.

Full Text

The Full Text of this article is available as a PDF (400.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abou-Zeid C., Harboe M., Rook G. A. Characterization of the secreted antigens of Mycobacterium bovis BCG: comparison of the 46-kilodalton dimeric protein with proteins MPB64 and MPB70. Infect Immun. 1987 Dec;55(12):3213–3214. doi: 10.1128/iai.55.12.3213-3214.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Abou-Zeid C., Smith I., Grange J., Steele J., Rook G. Subdivision of daughter strains of bacille Calmette-Guérin (BCG) according to secreted protein patterns. J Gen Microbiol. 1986 Nov;132(11):3047–3053. doi: 10.1099/00221287-132-11-3047. [DOI] [PubMed] [Google Scholar]
  3. Brosman S. A., Lamm D. L. The preparation, handling and use of intravesical bacillus Calmette-Guerin for the management of stage Ta, T1, carcinoma in situ and transitional cell cancer. J Urol. 1990 Aug;144(2 Pt 1):313–315. doi: 10.1016/s0022-5347(17)39441-7. [DOI] [PubMed] [Google Scholar]
  4. Collins D. M., De Lisle G. W. BCG identification by DNA restriction fragment patterns. J Gen Microbiol. 1987 Jun;133(6):1431–1434. doi: 10.1099/00221287-133-6-1431. [DOI] [PubMed] [Google Scholar]
  5. Das S., Paramasivan C. N., Lowrie D. B., Prabhakar R., Narayanan P. R. IS6110 restriction fragment length polymorphism typing of clinical isolates of Mycobacterium tuberculosis from patients with pulmonary tuberculosis in Madras, south India. Tuber Lung Dis. 1995 Dec;76(6):550–554. doi: 10.1016/0962-8479(95)90533-2. [DOI] [PubMed] [Google Scholar]
  6. Floyd M. M., Silcox V. A., Jones W. D., Jr, Butler W. R., Kilburn J. O. Separation of Mycobacterium bovis BCG from Mycobacterium tuberculosis and Mycobacterium bovis by using high-performance liquid chromatography of mycolic acids. J Clin Microbiol. 1992 May;30(5):1327–1330. doi: 10.1128/jcm.30.5.1327-1330.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fomukong N. G., Dale J. W., Osborn T. W., Grange J. M. Use of gene probes based on the insertion sequence IS986 to differentiate between BCG vaccine strains. J Appl Bacteriol. 1992 Feb;72(2):126–133. doi: 10.1111/j.1365-2672.1992.tb01813.x. [DOI] [PubMed] [Google Scholar]
  8. Frothingham R. Differentiation of strains in Mycobacterium tuberculosis complex by DNA sequence polymorphisms, including rapid identification of M. bovis BCG. J Clin Microbiol. 1995 Apr;33(4):840–844. doi: 10.1128/jcm.33.4.840-844.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hermans P. W., van Soolingen D., Bik E. M., de Haas P. E., Dale J. W., van Embden J. D. Insertion element IS987 from Mycobacterium bovis BCG is located in a hot-spot integration region for insertion elements in Mycobacterium tuberculosis complex strains. Infect Immun. 1991 Aug;59(8):2695–2705. doi: 10.1128/iai.59.8.2695-2705.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hermans P. W., van Soolingen D., Dale J. W., Schuitema A. R., McAdam R. A., Catty D., van Embden J. D. Insertion element IS986 from Mycobacterium tuberculosis: a useful tool for diagnosis and epidemiology of tuberculosis. J Clin Microbiol. 1990 Sep;28(9):2051–2058. doi: 10.1128/jcm.28.9.2051-2058.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Huh Y. J., Ahn D. I., Kim S. J. Limited variation of DNA fingerprints (IS6110 and IS1081) in Korean strains of Mycobacterium tuberculosis. Tuber Lung Dis. 1995 Aug;76(4):324–329. doi: 10.1016/s0962-8479(05)80031-0. [DOI] [PubMed] [Google Scholar]
  12. Kröger L., Korppi M., Brander E., Kröger H., Wasz-Höckert O., Backman A., Rapola J., Launiala K., Katila M. L. Osteitis caused by bacille Calmette-Guérin vaccination: a retrospective analysis of 222 cases. J Infect Dis. 1995 Aug;172(2):574–576. doi: 10.1093/infdis/172.2.574. [DOI] [PubMed] [Google Scholar]
  13. Li H., Ulstrup J. C., Jonassen T. O., Melby K., Nagai S., Harboe M. Evidence for absence of the MPB64 gene in some substrains of Mycobacterium bovis BCG. Infect Immun. 1993 May;61(5):1730–1734. doi: 10.1128/iai.61.5.1730-1734.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Miura K., Nagai S., Kinomoto M., Haga S., Tokunaga T. Comparative studies with various substrains of Mycobacterium bovis BCG on the production of an antigenic protein, MPB70. Infect Immun. 1983 Feb;39(2):540–545. doi: 10.1128/iai.39.2.540-545.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Osborn T. W. Changes in BCG strains. Tubercle. 1983 Mar;64(1):1–13. doi: 10.1016/0041-3879(83)90044-2. [DOI] [PubMed] [Google Scholar]
  16. Pansadoro V., Emiliozzi P., Defidio L., Donadio D., Florio A., Maurelli S., Lauretti S., Sternberg C. N. Bacillus Calmette-Guerin in the treatment of stage T1 grade 3 transitional cell carcinoma of the bladder: long-term results. J Urol. 1995 Dec;154(6):2054–2058. [PubMed] [Google Scholar]
  17. Small P. M., Hopewell P. C., Singh S. P., Paz A., Parsonnet J., Ruston D. C., Schecter G. F., Daley C. L., Schoolnik G. K. The epidemiology of tuberculosis in San Francisco. A population-based study using conventional and molecular methods. N Engl J Med. 1994 Jun 16;330(24):1703–1709. doi: 10.1056/NEJM199406163302402. [DOI] [PubMed] [Google Scholar]
  18. Zhang Y., Wallace R. J., Jr, Mazurek G. H. Genetic differences between BCG substrains. Tuber Lung Dis. 1995 Feb;76(1):43–50. doi: 10.1016/0962-8479(95)90579-0. [DOI] [PubMed] [Google Scholar]
  19. van Deutekom H., Smulders Y. M., Roozendaal K. J., van Soolingen D. Bacille Calmette-Guérin (BCG) meningitis in an AIDS patient 12 years after vaccination with BCG. Clin Infect Dis. 1996 May;22(5):870–871. doi: 10.1093/clinids/22.5.870. [DOI] [PubMed] [Google Scholar]
  20. van Embden J. D., Cave M. D., Crawford J. T., Dale J. W., Eisenach K. D., Gicquel B., Hermans P., Martin C., McAdam R., Shinnick T. M. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol. 1993 Feb;31(2):406–409. doi: 10.1128/jcm.31.2.406-409.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. van Soolingen D., Hermans P. W., de Haas P. E., Soll D. R., van Embden J. D. Occurrence and stability of insertion sequences in Mycobacterium tuberculosis complex strains: evaluation of an insertion sequence-dependent DNA polymorphism as a tool in the epidemiology of tuberculosis. J Clin Microbiol. 1991 Nov;29(11):2578–2586. doi: 10.1128/jcm.29.11.2578-2586.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. van Soolingen D., Hermans P. W., de Haas P. E., van Embden J. D. Insertion element IS1081-associated restriction fragment length polymorphisms in Mycobacterium tuberculosis complex species: a reliable tool for recognizing Mycobacterium bovis BCG. J Clin Microbiol. 1992 Jul;30(7):1772–1777. doi: 10.1128/jcm.30.7.1772-1777.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. van Soolingen D., de Haas P. E., Hermans P. W., Groenen P. M., van Embden J. D. Comparison of various repetitive DNA elements as genetic markers for strain differentiation and epidemiology of Mycobacterium tuberculosis. J Clin Microbiol. 1993 Aug;31(8):1987–1995. doi: 10.1128/jcm.31.8.1987-1995.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES