Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1997 Apr;35(4):1008–1010. doi: 10.1128/jcm.35.4.1008-1010.1997

A nosocomial outbreak due to Enterobacter cloacae strains with the E. hormaechei genotype in patients treated with fluoroquinolones.

A Davin-Regli 1, C Bosi 1, R Charrel 1, E Ageron 1, L Papazian 1, P A Grimont 1, A Cremieux 1, C Bollet 1
PMCID: PMC229724  PMID: 9157119

Abstract

During a 7-month period, we isolated 21 highly fluoroquinolone-resistant Enterobacter cloaecae strains in units from two hospitals in Marseille, France. Random amplification of polymorphic DNA showed clonal identity between isolates which, furthermore, presented the Enterobacter hormaechei genotype on DNA-DNA hybridization. The emergence of this clone was observed only in patients treated with fluoroquinolones.

Full Text

The Full Text of this article is available as a PDF (701.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acolet D., Ahmet Z., Houang E., Hurley R., Kaufmann M. E. Enterobacter cloacae in a neonatal intensive care unit: account of an outbreak and its relationship to use of third generation cephalosporins. J Hosp Infect. 1994 Dec;28(4):273–286. doi: 10.1016/0195-6701(94)90091-4. [DOI] [PubMed] [Google Scholar]
  2. Brenner D. J., McWhorter A. C., Knutson J. K., Steigerwalt A. G. Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds. J Clin Microbiol. 1982 Jun;15(6):1133–1140. doi: 10.1128/jcm.15.6.1133-1140.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Davin-Regli A., Bollet C., Auffray J. P., Saux P., De Micco P. Use of random amplified polymorphic DNA for epidemiological typing of Stenotrophomonas maltophilia. J Hosp Infect. 1996 Jan;32(1):39–50. doi: 10.1016/s0195-6701(96)90163-2. [DOI] [PubMed] [Google Scholar]
  4. Davin-Regli A., Monnet D., Saux P., Bosi C., Charrel R., Barthelemy A., Bollet C. Molecular epidemiology of Enterobacter aerogenes acquisition: one-year prospective study in two intensive care units. J Clin Microbiol. 1996 Jun;34(6):1474–1480. doi: 10.1128/jcm.34.6.1474-1480.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Davin-Regli A., Saux P., Bollet C., Gouin F., De Micco P. Investigation of outbreaks of Enterobacter aerogenes colonisation and infection in intensive care units by random amplification of polymorphic DNA. J Med Microbiol. 1996 Feb;44(2):89–98. doi: 10.1099/00222615-44-2-89. [DOI] [PubMed] [Google Scholar]
  6. Dupeyron C., Mangeney N., Sedrati L., Campillo B., Fouet P., Leluan G. Rapid emergence of quinolone resistance in cirrhotic patients treated with norfloxacin to prevent spontaneous bacterial peritonitis. Antimicrob Agents Chemother. 1994 Feb;38(2):340–344. doi: 10.1128/aac.38.2.340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Falkiner F. R. Enterobacter in hospital. J Hosp Infect. 1992 Mar;20(3):137–140. doi: 10.1016/0195-6701(92)90081-v. [DOI] [PubMed] [Google Scholar]
  8. Gaston M. A. Enterobacter: an emerging nosocomial pathogen. J Hosp Infect. 1988 Apr;11(3):197–208. doi: 10.1016/0195-6701(88)90098-9. [DOI] [PubMed] [Google Scholar]
  9. Georghiou P. R., Hamill R. J., Wright C. E., Versalovic J., Koeuth T., Watson D. A., Lupski J. R. Molecular epidemiology of infections due to Enterobacter aerogenes: identification of hospital outbreak-associated strains by molecular techniques. Clin Infect Dis. 1995 Jan;20(1):84–94. doi: 10.1093/clinids/20.1.84. [DOI] [PubMed] [Google Scholar]
  10. Grattard F., Pozzetto B., Berthelot P., Rayet I., Ros A., Lauras B., Gaudin O. G. Arbitrarily primed PCR, ribotyping, and plasmid pattern analysis applied to investigation of a nosocomial outbreak due to Enterobacter cloacae in a neonatal intensive care unit. J Clin Microbiol. 1994 Mar;32(3):596–602. doi: 10.1128/jcm.32.3.596-602.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Haertl R., Bandlow G. Epidemiological fingerprinting of Enterobacter cloacae by small-fragment restriction endonuclease analysis and pulsed-field gel electrophoresis of genomic restriction fragments. J Clin Microbiol. 1993 Jan;31(1):128–133. doi: 10.1128/jcm.31.1.128-133.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lehn N., Stower-Hoffmann J., Kott T., Strassner C., Wagner H., Kronke M., Schneider-Brachert W. Characterization of clinical isolates of Escherichia coli showing high levels of fluoroquinolone resistance. J Clin Microbiol. 1996 Mar;34(3):597–602. doi: 10.1128/jcm.34.3.597-602.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. O'Hara C. M., Steigerwalt A. G., Hill B. C., Farmer J. J., 3rd, Fanning G. R., Brenner D. J. Enterobacter hormaechei, a new species of the family Enterobacteriaceae formerly known as enteric group 75. J Clin Microbiol. 1989 Sep;27(9):2046–2049. doi: 10.1128/jcm.27.9.2046-2049.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shlaes D. M. The clinical relevance of Enterobacter infections. Clin Ther. 1993;15 (Suppl A):21–28. [PubMed] [Google Scholar]
  15. Weinstein R. A. Endemic emergence of cephalosporin-resistant Enterobacter: relation to prior therapy. Infect Control. 1986 Feb;7(2 Suppl):120–123. doi: 10.1017/s0195941700065632. [DOI] [PubMed] [Google Scholar]
  16. Williams J. G., Kubelik A. R., Livak K. J., Rafalski J. A., Tingey S. V. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990 Nov 25;18(22):6531–6535. doi: 10.1093/nar/18.22.6531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Woods C. R., Jr, Versalovic J., Koeuth T., Lupski J. R. Analysis of relationships among isolates of Citrobacter diversus by using DNA fingerprints generated by repetitive sequence-based primers in the polymerase chain reaction. J Clin Microbiol. 1992 Nov;30(11):2921–2929. doi: 10.1128/jcm.30.11.2921-2929.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES