Abstract
Salmonella pullorum is a host-adapted pathogen of poultry previously thought to be nonmotile and nonflagellated. We discovered that motility can be induced in this organism under special medium conditions, and this motility was observed in 39 of 44 S. pullorum isolates tested. The migration appeared to occur only on the medium surface and not within the medium itself, indicating that swimming may not be responsible for this event. Agar concentration, carbohydrate concentration and type, and temperature of incubation all affected the motility. Flagellar stains and transmission electron micrographs of the motile S. pullorum culture showed long fibrous appendages resembling flagella extending from the cells, but these appendages were thinner and less numerous than the flagella observed on Salmonella enteritidis. Antisera to G flagellar antigens reacted strongly with the induced-motility S. pullorum culture, indicating that G epitopes were expressed on these cells. These results indicate that, contrary to the paradigm which held that S. pullorum is nonmotile and nonflagellated, motility can be induced in S. pullorum and that the organism appears to have the capacity to produce flagella.
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Selected References
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- Alberti L., Harshey R. M. Differentiation of Serratia marcescens 274 into swimmer and swarmer cells. J Bacteriol. 1990 Aug;172(8):4322–4328. doi: 10.1128/jb.172.8.4322-4328.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Allison C., Hughes C. Bacterial swarming: an example of prokaryotic differentiation and multicellular behaviour. Sci Prog. 1991;75(298 Pt 3-4):403–422. [PubMed] [Google Scholar]
- Bullis K. L. The history of avian medicine in the U.S. II. Pullorum disease and fowl typhoid. Avian Dis. 1977 Jul-Sep;21(3):422–429. [PubMed] [Google Scholar]
- Girón J. A. Expression of flagella and motility by Shigella. Mol Microbiol. 1995 Oct;18(1):63–75. doi: 10.1111/j.1365-2958.1995.mmi_18010063.x. [DOI] [PubMed] [Google Scholar]
- Harshey R. M., Matsuyama T. Dimorphic transition in Escherichia coli and Salmonella typhimurium: surface-induced differentiation into hyperflagellate swarmer cells. Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8631–8635. doi: 10.1073/pnas.91.18.8631. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kilger G., Grimont P. A. Differentiation of Salmonella phase 1 flagellar antigen types by restriction of the amplified fliC gene. J Clin Microbiol. 1993 May;31(5):1108–1110. doi: 10.1128/jcm.31.5.1108-1110.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kodaka H., Armfield A. Y., Lombard G. L., Dowell V. R., Jr Practical procedure for demonstrating bacterial flagella. J Clin Microbiol. 1982 Nov;16(5):948–952. doi: 10.1128/jcm.16.5.948-952.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]