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. 1997 Jun;35(6):1327–1331. doi: 10.1128/jcm.35.6.1327-1331.1997

Prevalence of Bartonella henselae and Bartonella clarridgeiae in stray cats.

R Heller 1, M Artois 1, V Xemar 1, D De Briel 1, H Gehin 1, B Jaulhac 1, H Monteil 1, Y Piemont 1
PMCID: PMC229743  PMID: 9163438

Abstract

The aim of the present work was to determine by blood culture the prevalence of blood infection with Bartonella species in a well-defined, European, urban stray cat population. Therefore, 94 stray cats were trapped from 10 cat colonies. Blood samples of these cats were cultured on both blood agar and liquid medium in order to raise the likelihood of bacterial detection. Fifty blood samples (53%) gave a positive culture result for Bartonella species. Isolate identification was performed by sequencing the first 430 bases of the 16S ribosomal DNA. Three types of sequences were thus obtained. The first type (17 isolates; 34%) was identical to that of B. henselae Houston-1 and the corresponding strains were referred as B. henselae type I. The second sequence type (18 isolates; 36%) was identical to that initially described as "BA-TF," and the corresponding strains were referred to as B. henselae type II. The third sequence type (15 isolates; 30%) was identical to that of the Bartonella clarridgeiae type strain (ATCC 51734). Our study points out the major role of stray cats as a reservoir of Bartonella spp. which can be transmitted to pet cats and, consequently, to humans. The study also highlights the high prevalence of B. clarridgeiae (16%) in the blood of stray cats.

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Selected References

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  1. Anderson B., Sims K., Regnery R., Robinson L., Schmidt M. J., Goral S., Hager C., Edwards K. Detection of Rochalimaea henselae DNA in specimens from cat scratch disease patients by PCR. J Clin Microbiol. 1994 Apr;32(4):942–948. doi: 10.1128/jcm.32.4.942-948.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bergmans A. M., Groothedde J. W., Schellekens J. F., van Embden J. D., Ossewaarde J. M., Schouls L. M. Etiology of cat scratch disease: comparison of polymerase chain reaction detection of Bartonella (formerly Rochalimaea) and Afipia felis DNA with serology and skin tests. J Infect Dis. 1995 Apr;171(4):916–923. doi: 10.1093/infdis/171.4.916. [DOI] [PubMed] [Google Scholar]
  3. Bergmans A. M., Schellekens J. F., van Embden J. D., Schouls L. M. Predominance of two Bartonella henselae variants among cat-scratch disease patients in the Netherlands. J Clin Microbiol. 1996 Feb;34(2):254–260. doi: 10.1128/jcm.34.2.254-260.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chomel B. B., Abbott R. C., Kasten R. W., Floyd-Hawkins K. A., Kass P. H., Glaser C. A., Pedersen N. C., Koehler J. E. Bartonella henselae prevalence in domestic cats in California: risk factors and association between bacteremia and antibody titers. J Clin Microbiol. 1995 Sep;33(9):2445–2450. doi: 10.1128/jcm.33.9.2445-2450.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chomel B. B., Kasten R. W., Floyd-Hawkins K., Chi B., Yamamoto K., Roberts-Wilson J., Gurfield A. N., Abbott R. C., Pedersen N. C., Koehler J. E. Experimental transmission of Bartonella henselae by the cat flea. J Clin Microbiol. 1996 Aug;34(8):1952–1956. doi: 10.1128/jcm.34.8.1952-1956.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clarridge J. E., 3rd, Raich T. J., Pirwani D., Simon B., Tsai L., Rodriguez-Barradas M. C., Regnery R., Zollo A., Jones D. C., Rambo C. Strategy to detect and identify Bartonella species in routine clinical laboratory yields Bartonella henselae from human immunodeficiency virus-positive patient and unique Bartonella strain from his cat. J Clin Microbiol. 1995 Aug;33(8):2107–2113. doi: 10.1128/jcm.33.8.2107-2113.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Courchamp F., Pontier D. Feline immunodeficiency virus: an epidemiological review. C R Acad Sci III. 1994 Dec;317(12):1123–1134. [PubMed] [Google Scholar]
  8. Higgins D. G., Sharp P. M. CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene. 1988 Dec 15;73(1):237–244. doi: 10.1016/0378-1119(88)90330-7. [DOI] [PubMed] [Google Scholar]
  9. Joblet C., Roux V., Drancourt M., Gouvernet J., Raoult D. Identification of Bartonella (Rochalimaea) species among fastidious gram-negative bacteria on the basis of the partial sequence of the citrate-synthase gene. J Clin Microbiol. 1995 Jul;33(7):1879–1883. doi: 10.1128/jcm.33.7.1879-1883.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Koehler J. E., Glaser C. A., Tappero J. W. Rochalimaea henselae infection. A new zoonosis with the domestic cat as reservoir. JAMA. 1994 Feb 16;271(7):531–535. doi: 10.1001/jama.271.7.531. [DOI] [PubMed] [Google Scholar]
  11. Kordick D. L., Wilson K. H., Sexton D. J., Hadfield T. L., Berkhoff H. A., Breitschwerdt E. B. Prolonged Bartonella bacteremia in cats associated with cat-scratch disease patients. J Clin Microbiol. 1995 Dec;33(12):3245–3251. doi: 10.1128/jcm.33.12.3245-3251.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kwok S., Higuchi R. Avoiding false positives with PCR. Nature. 1989 May 18;339(6221):237–238. doi: 10.1038/339237a0. [DOI] [PubMed] [Google Scholar]
  13. Larson A. M., Dougherty M. J., Nowowiejski D. J., Welch D. F., Matar G. M., Swaminathan B., Coyle M. B. Detection of Bartonella (Rochalimaea) quintana by routine acridine orange staining of broth blood cultures. J Clin Microbiol. 1994 Jun;32(6):1492–1496. doi: 10.1128/jcm.32.6.1492-1496.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lutz H., Pedersen N. C., Durbin R., Theilen G. H. Monoclonal antibodies to three epitopic regions of feline leukemia virus p27 and their use in enzyme-linked immunosorbent assay of p27. J Immunol Methods. 1983 Jan 28;56(2):209–220. doi: 10.1016/0022-1759(83)90413-1. [DOI] [PubMed] [Google Scholar]
  15. Matar G. M. Inter- and intraspecies identification of Bartonella species by PCR-restriction fragment length polymorphism analysis of a fragment of the ribosomal operon. J Clin Microbiol. 1995 Dec;33(12):3370–3370. doi: 10.1128/jcm.33.12.3370-3370.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pedersen N. C., Yamamoto J. K., Ishida T., Hansen H. Feline immunodeficiency virus infection. Vet Immunol Immunopathol. 1989 May;21(1):111–129. doi: 10.1016/0165-2427(89)90134-7. [DOI] [PubMed] [Google Scholar]
  17. Regnery R. L., Anderson B. E., Clarridge J. E., 3rd, Rodriguez-Barradas M. C., Jones D. C., Carr J. H. Characterization of a novel Rochalimaea species, R. henselae sp. nov., isolated from blood of a febrile, human immunodeficiency virus-positive patient. J Clin Microbiol. 1992 Feb;30(2):265–274. doi: 10.1128/jcm.30.2.265-274.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Relman D. A., Lepp P. W., Sadler K. N., Schmidt T. M. Phylogenetic relationships among the agent of bacillary angiomatosis, Bartonella bacilliformis, and other alpha-proteobacteria. Mol Microbiol. 1992 Jul;6(13):1801–1807. doi: 10.1111/j.1365-2958.1992.tb01352.x. [DOI] [PubMed] [Google Scholar]
  19. Roux V., Raoult D. Inter- and intraspecies identification of Bartonella (Rochalimaea) species. J Clin Microbiol. 1995 Jun;33(6):1573–1579. doi: 10.1128/jcm.33.6.1573-1579.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Woese C. R. Bacterial evolution. Microbiol Rev. 1987 Jun;51(2):221–271. doi: 10.1128/mr.51.2.221-271.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Zangwill K. M., Hamilton D. H., Perkins B. A., Regnery R. L., Plikaytis B. D., Hadler J. L., Cartter M. L., Wenger J. D. Cat scratch disease in Connecticut. Epidemiology, risk factors, and evaluation of a new diagnostic test. N Engl J Med. 1993 Jul 1;329(1):8–13. doi: 10.1056/NEJM199307013290102. [DOI] [PubMed] [Google Scholar]
  22. de Lamballerie X., Zandotti C., Vignoli C., Bollet C., de Micco P. A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification. Res Microbiol. 1992 Oct;143(8):785–790. doi: 10.1016/0923-2508(92)90107-y. [DOI] [PubMed] [Google Scholar]

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