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. 1997 Jun;35(6):1465–1468. doi: 10.1128/jcm.35.6.1465-1468.1997

Prevalence of granulocytic Ehrlichia infection among white-tailed deer in Wisconsin.

E A Belongia 1, K D Reed 1, P D Mitchell 1, C P Kolbert 1, D H Persing 1, J S Gill 1, J J Kazmierczak 1
PMCID: PMC229768  PMID: 9163463

Abstract

Human granulocytic ehrlichiosis (HGE) is caused by an agent that is nearly indistinguishable from the veterinary pathogens Ehrlichia equi and Ehrlichia phagocytophila. The deer tick, Ixodes scapularis, is a vector of the HGE agent, and the white-tailed deer is the primary host for adult Ixodes ticks. We assessed the distribution of granulocytic Ehrlichia infection among deer living within (Wisconsin) and outside (western and southern Iowa) the geographic range of L. scapularis. Whole-blood samples were tested for HGE 16S ribosomal DNA (rDNA) by PCR, and E. equi antibody was detected by indirect immunofluorescence assay (IFA). Antibody titers of > or = 1:64 were defined as positive, and all positive samples were retested with a second lot of substrate antigen. E. equi antibody was present in 14 (8%) of 187 Wisconsin deer and 0 of 60 Iowa specimens (rate ratio undefined; P = 0.025). An additional 30 serum samples from Wisconsin deer were excluded because IFA results were discrepant between substrate lots. The reciprocal antibody titers ranged from 64 to 512 (geometric mean, 141) for positive samples. PCR results were positive for 27 (15%) of 181 Wisconsin deer. The prevalence of infection in northwestern Wisconsin deer was not significantly different from that in central Wisconsin deer, as determined by IFA and PCR. In two samples that were sequenced, the 16S rDNA was nearly identical to that of the granulocytic Ehrlichia species but distinct from that of Anaplasma marginale. The DNA sequences of the samples differed from the published sequences for E. equi, E. phagocytophila, and the HGE agent by 1 or 2 nucleotides (> or = 99.1% homology) at phylogenetically informative sites. Granulocytic Ehrlichia organisms in deer are widely distributed within the geographic range of L. scapularis in Wisconsin. Deer may serve as useful sentinels for areas where HGE transmission to humans may occur.

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Selected References

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  1. Anderson B. E., Sims K. G., Olson J. G., Childs J. E., Piesman J. F., Happ C. M., Maupin G. O., Johnson B. J. Amblyomma americanum: a potential vector of human ehrlichiosis. Am J Trop Med Hyg. 1993 Aug;49(2):239–244. doi: 10.4269/ajtmh.1993.49.239. [DOI] [PubMed] [Google Scholar]
  2. Anderson J. F., Duray P. H., Magnarelli L. A. Prevalence of Borrelia burgdorferi in white-footed mice and Ixodes dammini at Fort McCoy, Wis. J Clin Microbiol. 1987 Aug;25(8):1495–1497. doi: 10.1128/jcm.25.8.1495-1497.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bakken J. S., Dumler J. S., Chen S. M., Eckman M. R., Van Etta L. L., Walker D. H. Human granulocytic ehrlichiosis in the upper Midwest United States. A new species emerging? JAMA. 1994 Jul 20;272(3):212–218. [PubMed] [Google Scholar]
  4. Bakken J. S., Krueth J. K., Lund T., Malkovitch D., Asanovich K., Dumler J. S. Exposure to deer blood may be a cause of human granulocytic ehrlichiosis. Clin Infect Dis. 1996 Jul;23(1):198–198. doi: 10.1093/clinids/23.1.198. [DOI] [PubMed] [Google Scholar]
  5. Bakken J. S., Krueth J., Wilson-Nordskog C., Tilden R. L., Asanovich K., Dumler J. S. Clinical and laboratory characteristics of human granulocytic ehrlichiosis. JAMA. 1996 Jan 17;275(3):199–205. [PubMed] [Google Scholar]
  6. Barlough J. E., Madigan J. E., DeRock E., Dumler J. S., Bakken J. S. Protection against Ehrlichia equi is conferred by prior infection with the human granulocytotropic Ehrlichia (HGE agent). J Clin Microbiol. 1995 Dec;33(12):3333–3334. doi: 10.1128/jcm.33.12.3333-3334.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chen S. M., Dumler J. S., Bakken J. S., Walker D. H. Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease. J Clin Microbiol. 1994 Mar;32(3):589–595. doi: 10.1128/jcm.32.3.589-595.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dawson J. E., Warner C. K., Baker V., Ewing S. A., Stallknecht D. E., Davidson W. R., Kocan A. A., Lockhart J. M., Olson J. G. Ehrlichia-like 16S rDNA sequence from wild white-tailed deer (Odocoileus virginianus). J Parasitol. 1996 Feb;82(1):52–58. [PubMed] [Google Scholar]
  9. Eng T. R., Harkess J. R., Fishbein D. B., Dawson J. E., Greene C. N., Redus M. A., Satalowich F. T. Epidemiologic, clinical, and laboratory findings of human ehrlichiosis in the United States, 1988. JAMA. 1990 Nov 7;264(17):2251–2258. [PubMed] [Google Scholar]
  10. FOGGIE A. Studies on the infectious agent of tick-borne fever in sheep. J Pathol Bacteriol. 1951 Jan;63(1):1–15. doi: 10.1002/path.1700630103. [DOI] [PubMed] [Google Scholar]
  11. Goodman J. L., Nelson C., Vitale B., Madigan J. E., Dumler J. S., Kurtti T. J., Munderloh U. G. Direct cultivation of the causative agent of human granulocytic ehrlichiosis. N Engl J Med. 1996 Jan 25;334(4):209–215. doi: 10.1056/NEJM199601253340401. [DOI] [PubMed] [Google Scholar]
  12. Gribble D. H. Equine ehrlichiosis. J Am Vet Med Assoc. 1969 Jul 15;155(2):462–469. [PubMed] [Google Scholar]
  13. Krause P. J., Telford S. R., 3rd, Spielman A., Sikand V., Ryan R., Christianson D., Burke G., Brassard P., Pollack R., Peck J. Concurrent Lyme disease and babesiosis. Evidence for increased severity and duration of illness. JAMA. 1996 Jun 5;275(21):1657–1660. [PubMed] [Google Scholar]
  14. Lockhart J. M., Davidson W. R., Dawson J. E., Stallknecht D. E. Temporal association of Amblyomma americanum with the presence of Ehrlichia chaffeensis reactive antibodies in white-tailed deer. J Wildl Dis. 1995 Apr;31(2):119–124. doi: 10.7589/0090-3558-31.2.119. [DOI] [PubMed] [Google Scholar]
  15. Madigan J. E., Richter P. J., Jr, Kimsey R. B., Barlough J. E., Bakken J. S., Dumler J. S. Transmission and passage in horses of the agent of human granulocytic ehrlichiosis. J Infect Dis. 1995 Oct;172(4):1141–1144. doi: 10.1093/infdis/172.4.1141. [DOI] [PubMed] [Google Scholar]
  16. Pancholi P., Kolbert C. P., Mitchell P. D., Reed K. D., Jr, Dumler J. S., Bakken J. S., Telford S. R., 3rd, Persing D. H. Ixodes dammini as a potential vector of human granulocytic ehrlichiosis. J Infect Dis. 1995 Oct;172(4):1007–1012. doi: 10.1093/infdis/172.4.1007. [DOI] [PubMed] [Google Scholar]
  17. Reed K. D., Mitchell P. D., Belongia E. A. Laboratory diagnosis of tick-borne diseases in Wisconsin. Wis Med J. 1996 Aug;95(8):557–563. [PubMed] [Google Scholar]
  18. Telford S. R., 3rd, Dawson J. E., Katavolos P., Warner C. K., Kolbert C. P., Persing D. H. Perpetuation of the agent of human granulocytic ehrlichiosis in a deer tick-rodent cycle. Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):6209–6214. doi: 10.1073/pnas.93.12.6209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Walker D. H., Dumler J. S. Emergence of the ehrlichioses as human health problems. Emerg Infect Dis. 1996 Jan-Mar;2(1):18–29. doi: 10.3201/eid0201.960102. [DOI] [PMC free article] [PubMed] [Google Scholar]

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