Abstract
We compared the capability of rapid enzyme immunoassay (EIA) to detect antiamoebic antibodies during hepatic amoebiasis with those of indirect hemagglutination and latex agglutination. EIA is simple to perform and rapid (20 min) and does not require any special equipment (optical reading is sufficient). EIA of 143 sera (including 43 from patients with proven hepatic amoebic abscess, 33 from patients with other hepatic disorders and/or parasitic infections, and 67 from healthy individuals) yielded a specificity, a sensitivity, and positive and negative predictive values of 100, 93, 100, and 97.1, respectively. This test could thus be considered another valuable tool for the diagnosis of hepatic amoebiasis.
Full Text
The Full Text of this article is available as a PDF (103.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Boisseau H., Appriou M., Lucchese F., Cabannes A., Tribouley-Duret J., Tribouley J. Evaluation d'un test d'hémagglutination indirecte amibiase (HAI) pour le serodiagnostic de l'amibiase. Med Trop (Mars) 1992 Jul-Sep;52(3):307–311. [PubMed] [Google Scholar]
- Gandhi B. M., Irshad M., Chawla T. C., Tandon B. N. Enzyme linked protein-A: an ELISA for detection of amoebic antibody. Trans R Soc Trop Med Hyg. 1987;81(2):183–185. doi: 10.1016/0035-9203(87)90207-0. [DOI] [PubMed] [Google Scholar]
- Hartmann D. P., Ghadirian E., Meerovitch E. Enzyme-linked immunosorbent assay (ELISA) and indirect hemagglutination (IHA) test in the serodiagnosis of experimental hepatic amebiasis. J Parasitol. 1980 Apr;66(2):344–345. [PubMed] [Google Scholar]
- Jacquier P., Gottstein B., Stingelin Y., Eckert J. Immunodiagnosis of toxocarosis in humans: evaluation of a new enzyme-linked immunosorbent assay kit. J Clin Microbiol. 1991 Sep;29(9):1831–1835. doi: 10.1128/jcm.29.9.1831-1835.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kozinn P. J., Taschdjian C. L., Goldberg P. K., Protzmann W. P., MacKenzie D. W., Remington J. S., Anderson S., Seelig M. S. Efficiency of serologic tests in the diagnosis of systemic candidiasis. Am J Clin Pathol. 1978 Dec;70(6):893–898. doi: 10.1093/ajcp/70.6.893. [DOI] [PubMed] [Google Scholar]
- Maddison S. E. Serodiagnosis of parasitic diseases. Clin Microbiol Rev. 1991 Oct;4(4):457–469. doi: 10.1128/cmr.4.4.457. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pahuchonma W., Janitschke K. Serodiagnosis of parasitic infections by ELISA with different antigens. Southeast Asian J Trop Med Public Health. 1987 Jun;18(2):193–196. [PubMed] [Google Scholar]
- Ravdin J. I. Amebiasis. Clin Infect Dis. 1995 Jun;20(6):1453–1466. doi: 10.1093/clinids/20.6.1453. [DOI] [PubMed] [Google Scholar]
- Robert R., Mahaza C., Bernard C., Buffard C., Senet J. M. Evaluation of a new bicolored latex agglutination test for immunological diagnosis of hepatic amoebiasis. J Clin Microbiol. 1990 Jun;28(6):1422–1424. doi: 10.1128/jcm.28.6.1422-1424.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sathar M. A., Simjee A. E., Nel J. D., Bredenkamp B. L., Gathiram V., Jackson T. F. Evaluation of an enzyme-linked immunosorbent assay in the serodiagnosis of amoebic liver abscess. S Afr Med J. 1988 Dec 17;74(12):625–628. [PubMed] [Google Scholar]
- Sloan L., Schneider S., Rosenblatt J. Evaluation of enzyme-linked immunoassay for serological diagnosis of cysticercosis. J Clin Microbiol. 1995 Dec;33(12):3124–3128. doi: 10.1128/jcm.33.12.3124-3128.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yang J., Kennedy M. T. Evaluation of enzyme-linked immunosorbent assay for the serodiagnosis of amebiasis. J Clin Microbiol. 1979 Dec;10(6):778–785. doi: 10.1128/jcm.10.6.778-785.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
