Abstract
Restriction enzyme analysis of PCR-amplified 16S rRNA genes was used to distinguish among clinical isolates of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, and Haemophilus paraphrophilus which were originally identified by conventional phenotypic methods. This PCR-based method is a reliable and rapid alternative to conventional methods for identification of these bacterial species.
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- Brondz I., Olsen I. Multivariate analyses of carbohydrate data from lipopolysaccharides of Actinobacillus (Haemophilus) actinomycetemcomitans, Haemophilus aphrophilus, and Haemophilus paraphrophilus. Int J Syst Bacteriol. 1990 Oct;40(4):405–408. doi: 10.1099/00207713-40-4-405. [DOI] [PubMed] [Google Scholar]
- Caugant D. A., Selander R. K., Olsen I. Differentiation between Actinobacillus (Haemophilus) actinomycetemcomitans, Haemophilus aphrophilus and Haemophilus paraphrophilus by multilocus enzyme electrophoresis. J Gen Microbiol. 1990 Oct;136(10):2135–2141. doi: 10.1099/00221287-136-10-2135. [DOI] [PubMed] [Google Scholar]
- Chadwick P. R., Malnick H., Ebizie A. O. Haemophilus paraphrophilus infection: a pitfall in laboratory diagnosis. J Infect. 1995 Jan;30(1):67–69. doi: 10.1016/s0163-4453(95)92977-0. [DOI] [PubMed] [Google Scholar]
- Christersson L. A., Fransson C. L., Dunford R. G., Zambon J. J. Subgingival distribution of periodontal pathogenic microorganisms in adult periodontitis. J Periodontol. 1992 May;63(5):418–425. doi: 10.1902/jop.1992.63.5.418. [DOI] [PubMed] [Google Scholar]
- Chuba P. J., Pelz K., Krekeler G., de Isele T. S., Göbel U. Synthetic oligodeoxynucleotide probes for the rapid detection of bacteria associated with human periodontitis. J Gen Microbiol. 1988 Jul;134(7):1931–1938. doi: 10.1099/00221287-134-7-1931. [DOI] [PubMed] [Google Scholar]
- Dewhirst F. E., Paster B. J., Olsen I., Fraser G. J. Phylogeny of 54 representative strains of species in the family Pasteurellaceae as determined by comparison of 16S rRNA sequences. J Bacteriol. 1992 Mar;174(6):2002–2013. doi: 10.1128/jb.174.6.2002-2013.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dix K., Watanabe S. M., McArdle S., Lee D. I., Randolph C., Moncla B., Schwartz D. E. Species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria. J Clin Microbiol. 1990 Feb;28(2):319–323. doi: 10.1128/jcm.28.2.319-323.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ebersole J. L., Sandoval M. N., Steffen M. J., Cappelli D. Serum antibody in Actinobacillus actinomycetemcomitans-infected patients with periodontal disease. Infect Immun. 1991 May;59(5):1795–1802. doi: 10.1128/iai.59.5.1795-1802.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kilian M., Schiott C. R. Haemophili and related bacteria in the human oral cavity. Arch Oral Biol. 1975 Dec;20(12):791–796. doi: 10.1016/0003-9969(75)90055-2. [DOI] [PubMed] [Google Scholar]
- Myhrvold V., Brondz I., Olsen I. Application of multivariate analyses of enzymic data to classification of members of the Actinobacillus-Haemophilus-Pasteurella group. Int J Syst Bacteriol. 1992 Jan;42(1):12–18. doi: 10.1099/00207713-42-1-12. [DOI] [PubMed] [Google Scholar]
- Nieminen A., Kari K., Saxén L. Specific antibodies against Actinobacillus actinomycetemcomitans in serum and saliva of patients with advanced periodontitis. Scand J Dent Res. 1993 Aug;101(4):196–201. doi: 10.1111/j.1600-0722.1993.tb01104.x. [DOI] [PubMed] [Google Scholar]
- Preus H. R., Sunday G. J., Haraszthy V. I., Zambon J. J. Rapid identification of Actinobacillus actinomycetemcomitans based on analysis of 23S ribosomal RNA. Oral Microbiol Immunol. 1992 Dec;7(6):372–375. doi: 10.1111/j.1399-302x.1992.tb00639.x. [DOI] [PubMed] [Google Scholar]
- Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. doi: 10.1126/science.2448875. [DOI] [PubMed] [Google Scholar]
- Sandholm L., Tolo K., Olsen I. Salivary IgG, a parameter of periodontal disease activity? High responders to Actinobacillus actinomycetemcomitans Y4 in juvenile and adult periodontitis. J Clin Periodontol. 1987 May;14(5):289–294. doi: 10.1111/j.1600-051x.1987.tb01535.x. [DOI] [PubMed] [Google Scholar]
- Sedlácek I., Gerner-Smidt P., Schmidt J., Frederiksen W. Genetic relationship of strains of Haemophilus aphrophilus, H. paraphrophilus, and Actinobacillus actinomycetemcomitans studied by ribotyping. Zentralbl Bakteriol. 1993 Jun;279(1):51–59. doi: 10.1016/s0934-8840(11)80491-3. [DOI] [PubMed] [Google Scholar]
- Slots J. Bacterial specificity in adult periodontitis. A summary of recent work. J Clin Periodontol. 1986 Nov;13(10):912–917. doi: 10.1111/j.1600-051x.1986.tb01426.x. [DOI] [PubMed] [Google Scholar]
- Slots J., Reynolds H. S., Genco R. J. Actinobacillus actinomycetemcomitans in human periodontal disease: a cross-sectional microbiological investigation. Infect Immun. 1980 Sep;29(3):1013–1020. doi: 10.1128/iai.29.3.1013-1020.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Slots J. Salient Biochemical Characters of Actinobacillus actinomycetemcomitans. Arch Microbiol. 1982 Feb;131(1):60–67. doi: 10.1007/BF00451500. [DOI] [PubMed] [Google Scholar]
- Slots J. Selective medium for isolation of Actinobacillus actinomycetemcomitans. J Clin Microbiol. 1982 Apr;15(4):606–609. doi: 10.1128/jcm.15.4.606-609.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tanner A. C., Visconti R. A., Socransky S. S., Holt S. C. Classification and identification of Actinobacillus actinomycetemcomitans and haemophilus aphrophilus by cluster analysis and deoxyribonucleic acid hybridizations. J Periodontal Res. 1982 Nov;17(6):585–596. doi: 10.1111/j.1600-0765.1982.tb01180.x. [DOI] [PubMed] [Google Scholar]
- Tønjum T., Bukholm G., Bøvre K. Identification of Haemophilus aphrophilus and Actinobacillus actinomycetemcomitans by DNA-DNA hybridization and genetic transformation. J Clin Microbiol. 1990 Sep;28(9):1994–1998. doi: 10.1128/jcm.28.9.1994-1998.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tønjum T., Haas R. Identification of Actinobacillus actinomycetemcomitans by leukotoxin gene-specific hybridization and polymerase chain reaction assays. J Clin Microbiol. 1993 Jul;31(7):1856–1859. doi: 10.1128/jcm.31.7.1856-1859.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zambon J. J. Actinobacillus actinomycetemcomitans in human periodontal disease. J Clin Periodontol. 1985 Jan;12(1):1–20. doi: 10.1111/j.1600-051x.1985.tb01348.x. [DOI] [PubMed] [Google Scholar]
- Zambon J. J., Christersson L. A., Slots J. Actinobacillus actinomycetemcomitans in human periodontal disease. Prevalence in patient groups and distribution of biotypes and serotypes within families. J Periodontol. 1983 Dec;54(12):707–711. doi: 10.1902/jop.1983.54.12.707. [DOI] [PubMed] [Google Scholar]