Abstract
The chromosomal gene yst, which encodes a heat-stable enterotoxin of Yersinia enterocolitica, is a useful diagnostic marker because it occurs only in invasive strains of this species. A homologous gene also occurs in some strains of Yersinia kristensenii. Sequence analysis of the yst genes from two different strains of Y. enterocolitica and from Y. kristensenii revealed a substantial number of mismatches at the 3' ends of the yst genes of the so-called American and European biotypes of Y. enterocolitica. Moreover, several mismatches and a deletion of 5 codons were found in the yst of Y. kristensenii. These findings were used to develop a PCR-based assay for yst of Y. enterocolitica which yielded a detectable product in as little as 50 min. The assay was 100% specific in terms of its ability to identify potentially pathogenic strains of Y. enterocolitica regardless of biotype or serotype. The PCR yielded an amplicon that was visible on agarose gel electrophoresis from as few as 100 CFU, or 10 CFU when the PCR was combined with dot blot hybridization with a digoxigenin-labeled oligonucleotide probe that corresponded to an internal sequence of yst. These results establish the value of the yst gene as a target for the identification of pathogenic bioserotypes of Y. enterocolitica and the usefulness of PCR for this purpose.
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Selected References
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- Cornelis G. R. Yersinia pathogenicity factors. Curr Top Microbiol Immunol. 1994;192:243–263. doi: 10.1007/978-3-642-78624-2_11. [DOI] [PubMed] [Google Scholar]
- Cover T. L., Aber R. C. Yersinia enterocolitica. N Engl J Med. 1989 Jul 6;321(1):16–24. doi: 10.1056/NEJM198907063210104. [DOI] [PubMed] [Google Scholar]
- Delor I., Kaeckenbeeck A., Wauters G., Cornelis G. R. Nucleotide sequence of yst, the Yersinia enterocolitica gene encoding the heat-stable enterotoxin, and prevalence of the gene among pathogenic and nonpathogenic yersiniae. Infect Immun. 1990 Sep;58(9):2983–2988. doi: 10.1128/iai.58.9.2983-2988.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ibrahim A., Liesack W., Pike S., Stackebrandt E. The polymerase chain reaction: an epidemiological tool to differentiate between two clusters of pathogenic Yersinia enterocolitica strains. FEMS Microbiol Lett. 1992 Oct 1;76(1-2):63–66. doi: 10.1016/0378-1097(92)90364-t. [DOI] [PubMed] [Google Scholar]
- Ibrahim A., Liesack W., Stackebrandt E. Differentiation between pathogenic and non-pathogenic Yersinia enterocolitica strains by colony hybridization with a PCR-mediated digoxigenin-dUTP-labelled probe. Mol Cell Probes. 1992 Apr;6(2):163–171. doi: 10.1016/0890-8508(92)90061-2. [DOI] [PubMed] [Google Scholar]
- Ibrahim A., Liesack W., Stackebrandt E. Polymerase chain reaction-gene probe detection system specific for pathogenic strains of Yersinia enterocolitica. J Clin Microbiol. 1992 Aug;30(8):1942–1947. doi: 10.1128/jcm.30.8.1942-1947.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robins-Browne R. M., Cianciosi S., Bordun A. M., Wauters G. Pathogenicity of Yersinia kristensenii for mice. Infect Immun. 1991 Jan;59(1):162–167. doi: 10.1128/iai.59.1.162-167.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robins-Browne R. M., Still C. S., Miliotis M. D., Koornhof H. J. Mechanism of action of Yersinia enterocolitica enterotoxin. Infect Immun. 1979 Aug;25(2):680–684. doi: 10.1128/iai.25.2.680-684.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Takao T., Tominaga N., Shimonishi Y., Hara S., Inoue T., Miyama A. Primary structure of heat-stable enterotoxin produced by Yersinia enterocolitica. Biochem Biophys Res Commun. 1984 Dec 28;125(3):845–851. doi: 10.1016/0006-291x(84)91360-3. [DOI] [PubMed] [Google Scholar]