Abstract
The prevalence of the plasmid-encoded virulence-associated gene (vapA) of Rhodococcus equi, as determined by PCR, was found to be 98% in isolates from 154 foals with pneumonia, confirming the strong association of vapA with virulence. The vapA genes from 60 representative isolates were compared by digestion with the restriction endonuclease HinfI, and no evidence of sequence variation was detected.
Full Text
The Full Text of this article is available as a PDF (354.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Elissalde G. S., Renshaw H. W., Walberg J. A. Corynebacterium equi: an interhost review with emphasis on the foal. Comp Immunol Microbiol Infect Dis. 1980;3(4):433–445. doi: 10.1016/0147-9571(80)90018-1. [DOI] [PubMed] [Google Scholar]
- Hondalus M. K., Mosser D. M. Survival and replication of Rhodococcus equi in macrophages. Infect Immun. 1994 Oct;62(10):4167–4175. doi: 10.1128/iai.62.10.4167-4175.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sekizaki T., Takai S., Egawa Y., Ikeda T., Ito H., Tsubaki S. Sequence of the Rhodococcus equi gene encoding the virulence-associated 15-17-kDa antigens. Gene. 1995 Mar 21;155(1):135–136. doi: 10.1016/0378-1119(95)00009-u. [DOI] [PubMed] [Google Scholar]
- Takai S., Ikeda T., Sasaki Y., Watanabe Y., Ozawa T., Tsubaki S., Sekizaki T. Identification of virulent Rhodococcus equi by amplification of gene coding for 15- to 17-kilodalton antigens. J Clin Microbiol. 1995 Jun;33(6):1624–1627. doi: 10.1128/jcm.33.6.1624-1627.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Takai S., Koike K., Ohbushi S., Izumi C., Tsubaki S. Identification of 15- to 17-kilodalton antigens associated with virulent Rhodococcus equi. J Clin Microbiol. 1991 Mar;29(3):439–443. doi: 10.1128/jcm.29.3.439-443.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Takai S., Sasaki Y., Ikeda T., Uchida Y., Tsubaki S., Sekizaki T. Virulence of Rhodococcus equi isolates from patients with and without AIDS. J Clin Microbiol. 1994 Feb;32(2):457–460. doi: 10.1128/jcm.32.2.457-460.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Takai S., Sekizaki T., Ozawa T., Sugawara T., Watanabe Y., Tsubaki S. Association between a large plasmid and 15- to 17-kilodalton antigens in virulent Rhodococcus equi. Infect Immun. 1991 Nov;59(11):4056–4060. doi: 10.1128/iai.59.11.4056-4060.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Takai S., Sugawara T., Watanabe Y., Sasaki Y., Tsubaki S., Sekizaki T. Effect of growth temperature on maintenance of virulent Rhodococcus equi. Vet Microbiol. 1994 Mar;39(1-2):187–192. doi: 10.1016/0378-1135(94)90099-x. [DOI] [PubMed] [Google Scholar]
- Tan C., Prescott J. F., Patterson M. C., Nicholson V. M. Molecular characterization of a lipid-modified virulence-associated protein of Rhodococcus equi and its potential in protective immunity. Can J Vet Res. 1995 Jan;59(1):51–59. [PMC free article] [PubMed] [Google Scholar]
- Tkachuk-Saad O., Prescott J. Rhodococcus equi plasmids: isolation and partial characterization. J Clin Microbiol. 1991 Dec;29(12):2696–2700. doi: 10.1128/jcm.29.12.2696-2700.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
