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. 1997 Jul;35(7):1671–1674. doi: 10.1128/jcm.35.7.1671-1674.1997

Identification of Aeromonas clinical isolates by restriction fragment length polymorphism of PCR-amplified 16S rRNA genes.

N Borrell 1, S G Acinas 1, M J Figueras 1, A J Martínez-Murcia 1
PMCID: PMC229819  PMID: 9196171

Abstract

Identification of Aeromonas species, emergent pathogens for humans, has long been controversial due to their phenotypic and genomic heterogeneities. Computer analysis of the published 16S rRNA gene sequences revealed that restriction fragment length polymorphism of the PCR-amplified 16S rRNA gene is a good and rapid way of assessing the identities of all known species of Aeromonas. The method was evaluated with the reference strains of all species (or DNA homology groups) and 76 clinical isolates of diverse origin. Most results from the two approaches were in agreement, but some discrepancies were discerned. Advantages over previous phenotypic and genetic methods are discussed.

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Selected References

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  1. Abbott S. L., Cheung W. K., Kroske-Bystrom S., Malekzadeh T., Janda J. M. Identification of Aeromonas strains to the genospecies level in the clinical laboratory. J Clin Microbiol. 1992 May;30(5):1262–1266. doi: 10.1128/jcm.30.5.1262-1266.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Altwegg M., Altwegg-Bissig R., Demarta A., Peduzzi R., Reeves M. W., Swaminathan B. Comparison of four typing methods for Aeromonas species. J Diarrhoeal Dis Res. 1988 Jun;6(2):88–94. [PubMed] [Google Scholar]
  3. Altwegg M., Reeves M. W., Altwegg-Bissig R., Brenner D. J. Multilocus enzyme analysis of the genus Aeromonas and its use for species identification. Zentralbl Bakteriol. 1991 Apr;275(1):28–45. doi: 10.1016/s0934-8840(11)80765-6. [DOI] [PubMed] [Google Scholar]
  4. Ash C., Martinez-Murcia A. J., Collins M. D. Identification of Aeromonas schubertii and Aeromonas jandaei by using a polymerase chain reaction-probe test. FEMS Microbiol Lett. 1993 Apr 1;108(2):151–155. doi: 10.1111/j.1574-6968.1993.tb06091.x. [DOI] [PubMed] [Google Scholar]
  5. Carnahan A. M., Behram S., Joseph S. W. Aerokey II: a flexible key for identifying clinical Aeromonas species. J Clin Microbiol. 1991 Dec;29(12):2843–2849. doi: 10.1128/jcm.29.12.2843-2849.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Carnahan A. M., Chakraborty T., Fanning G. R., Verma D., Ali A., Janda J. M., Joseph S. W. Aeromonas trota sp. nov., an ampicillin-susceptible species isolated from clinical specimens. J Clin Microbiol. 1991 Jun;29(6):1206–1210. doi: 10.1128/jcm.29.6.1206-1210.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chopra A. K., Houston C. W., Peterson J. W., Jin G. F. Cloning, expression, and sequence analysis of a cytolytic enterotoxin gene from Aeromonas hydrophila. Can J Microbiol. 1993 May;39(5):513–523. doi: 10.1139/m93-073. [DOI] [PubMed] [Google Scholar]
  8. Collins M. D., Martinez-Murcia A. J., Cai J. Aeromonas enteropelogenes and Aeromonas ichthiosmia are identical to Aeromonas trota and Aeromonas veronii, respectively, as revealed by small-subunit rRNA sequence analysis. Int J Syst Bacteriol. 1993 Oct;43(4):855–856. doi: 10.1099/00207713-43-4-855. [DOI] [PubMed] [Google Scholar]
  9. Dorsch M., Ashbolt N. J., Cox P. T., Goodman A. E. Rapid identification of Aeromonas species using 16S rDNA targeted oligonucleotide primers: a molecular approach based on screening of environmental isolates. J Appl Bacteriol. 1994 Dec;77(6):722–726. doi: 10.1111/j.1365-2672.1994.tb02825.x. [DOI] [PubMed] [Google Scholar]
  10. Esteve C., Gutiérrez M. C., Ventosa A. Aeromonas encheleia sp. nov., isolated from European eels. Int J Syst Bacteriol. 1995 Jul;45(3):462–466. doi: 10.1099/00207713-45-3-462. [DOI] [PubMed] [Google Scholar]
  11. García-Martínez J., Martínez-Murcia A. J., Rodríguez-Valera F., Zorraquino A. Molecular evidence supporting the existence of two major groups in uropathogenic Escherichia coli. FEMS Immunol Med Microbiol. 1996 Jul;14(4):231–244. doi: 10.1111/j.1574-695X.1996.tb00291.x. [DOI] [PubMed] [Google Scholar]
  12. Hickman-Brenner F. W., Fanning G. R., Arduino M. J., Brenner D. J., Farmer J. J., 3rd Aeromonas schubertii, a new mannitol-negative species found in human clinical specimens. J Clin Microbiol. 1988 Aug;26(8):1561–1564. doi: 10.1128/jcm.26.8.1561-1564.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hickman-Brenner F. W., MacDonald K. L., Steigerwalt A. G., Fanning G. R., Brenner D. J., Farmer J. J., 3rd Aeromonas veronii, a new ornithine decarboxylase-positive species that may cause diarrhea. J Clin Microbiol. 1987 May;25(5):900–906. doi: 10.1128/jcm.25.5.900-906.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Huys G., Coopman R., Janssen P., Kersters K. High-resolution genotypic analysis of the genus Aeromonas by AFLP fingerprinting. Int J Syst Bacteriol. 1996 Apr;46(2):572–580. doi: 10.1099/00207713-46-2-572. [DOI] [PubMed] [Google Scholar]
  15. Janda J. M., Abbott S. L., Khashe S., Kellogg G. H., Shimada T. Further studies on biochemical characteristics and serologic properties of the genus Aeromonas. J Clin Microbiol. 1996 Aug;34(8):1930–1933. doi: 10.1128/jcm.34.8.1930-1933.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Janda J. M. Recent advances in the study of the taxonomy, pathogenicity, and infectious syndromes associated with the genus Aeromonas. Clin Microbiol Rev. 1991 Oct;4(4):397–410. doi: 10.1128/cmr.4.4.397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kuijper E. J., Steigerwalt A. G., Schoenmakers B. S., Peeters M. F., Zanen H. C., Brenner D. J. Phenotypic characterization and DNA relatedness in human fecal isolates of Aeromonas spp. J Clin Microbiol. 1989 Jan;27(1):132–138. doi: 10.1128/jcm.27.1.132-138.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kämpfer P., Blasczyk K., Auling G. Characterization of Aeromonas genomic species by using quinone, polyamine, and fatty acid patterns. Can J Microbiol. 1994 Oct;40(10):844–850. doi: 10.1139/m94-134. [DOI] [PubMed] [Google Scholar]
  19. Martinetti Lucchini G., Altwegg M. rRNA gene restriction patterns as taxonomic tools for the genus Aeromonas. Int J Syst Bacteriol. 1992 Jul;42(3):384–389. doi: 10.1099/00207713-42-3-384. [DOI] [PubMed] [Google Scholar]
  20. Martinez-Murcia A. J., Benlloch S., Collins M. D. Phylogenetic interrelationships of members of the genera Aeromonas and Plesiomonas as determined by 16S ribosomal DNA sequencing: lack of congruence with results of DNA-DNA hybridizations. Int J Syst Bacteriol. 1992 Jul;42(3):412–421. doi: 10.1099/00207713-42-3-412. [DOI] [PubMed] [Google Scholar]
  21. Martinez-Murcia A. J., Esteve C., Garay E., Collins M. D. Aeromonas allosaccharophila sp. nov., a new mesophilic member of the genus Aeromonas. FEMS Microbiol Lett. 1992 Mar 15;70(3):199–205. doi: 10.1016/0378-1097(92)90698-n. [DOI] [PubMed] [Google Scholar]
  22. Millership S. E., Want S. V. Characterisation of strains of Aeromonas spp. by phenotype and whole-cell protein fingerprint. J Med Microbiol. 1993 Aug;39(2):107–113. doi: 10.1099/00222615-39-2-107. [DOI] [PubMed] [Google Scholar]
  23. Oakey H. J., Ellis J. T., Gibson L. F. Differentiation of Aeromonas genomospecies using random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR). J Appl Bacteriol. 1996 Apr;80(4):402–410. doi: 10.1111/j.1365-2672.1996.tb03235.x. [DOI] [PubMed] [Google Scholar]
  24. Rossolini G. M., Zanchi A., Chiesurin A., Amicosante G., Satta G., Guglielmetti P. Distribution of cphA or related carbapenemase-encoding genes and production of carbapenemase activity in members of the genus Aeromonas. Antimicrob Agents Chemother. 1995 Feb;39(2):346–349. doi: 10.1128/aac.39.2.346. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Stephenson J. R., Millership S. E., Tabaqchali S. Typing of Aeromonas species by polyacrylamide-gel electrophoresis of radiolabelled cell proteins. J Med Microbiol. 1987 Sep;24(2):113–118. doi: 10.1099/00222615-24-2-113. [DOI] [PubMed] [Google Scholar]
  26. Woese C. R. Bacterial evolution. Microbiol Rev. 1987 Jun;51(2):221–271. doi: 10.1128/mr.51.2.221-271.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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