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. 1997 Jul;35(7):1870–1872. doi: 10.1128/jcm.35.7.1870-1872.1997

Determination of genotypes of hepatitis C virus in Venezuela by restriction fragment length polymorphism.

F H Pujol 1, C L Loureiro 1, M Devesa 1, L Blitz 1, K Parra 1, S Beker 1, F Liprandi 1
PMCID: PMC229860  PMID: 9196212

Abstract

Hepatitis C virus genotypes in Venezuela were analyzed by restriction fragment length polymorphism in the 5' noncoding region. The absence of BstUI digestion was found to be a useful marker for genotype 2 specimens. From 122 serum samples, 66, 20, and 2.5% were classified as genotypes 1, 2, and 3, respectively; 0.8% were classified as genotype 4; and 10% appeared to be mixed infections.

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Selected References

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  1. Davidson F., Simmonds P., Ferguson J. C., Jarvis L. M., Dow B. C., Follett E. A., Seed C. R., Krusius T., Lin C., Medgyesi G. A. Survey of major genotypes and subtypes of hepatitis C virus using RFLP of sequences amplified from the 5' non-coding region. J Gen Virol. 1995 May;76(Pt 5):1197–1204. doi: 10.1099/0022-1317-76-5-1197. [DOI] [PubMed] [Google Scholar]
  2. Dusheiko G., Schmilovitz-Weiss H., Brown D., McOmish F., Yap P. L., Sherlock S., McIntyre N., Simmonds P. Hepatitis C virus genotypes: an investigation of type-specific differences in geographic origin and disease. Hepatology. 1994 Jan;19(1):13–18. [PubMed] [Google Scholar]
  3. Holland P. V., Barrera J. M., Ercilla M. G., Yoshida C. F., Wang Y., de Olim G. A., Betlach B., Kuramoto K., Okamoto H. Genotyping hepatitis C virus isolates from Spain, Brazil, China, and Macau by a simplified PCR method. J Clin Microbiol. 1996 Oct;34(10):2372–2378. doi: 10.1128/jcm.34.10.2372-2378.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kleter G. E., van Doorn L. J., Brouwer J. T., Schalm S. W., Heijtink R. A., Quint W. G. Sequence analysis of the 5' untranslated region in isolates of at least four genotypes of hepatitis C virus in The Netherlands. J Clin Microbiol. 1994 Feb;32(2):306–310. doi: 10.1128/jcm.32.2.306-310.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lau J. Y., Mizokami M., Kolberg J. A., Davis G. L., Prescott L. E., Ohno T., Perrillo R. P., Lindsay K. L., Gish R. G., Qian K. P. Application of six hepatitis C virus genotyping systems to sera from chronic hepatitis C patients in the United States. J Infect Dis. 1995 Feb;171(2):281–289. doi: 10.1093/infdis/171.2.281. [DOI] [PubMed] [Google Scholar]
  6. Lee D. S., Sung Y. C., Whang Y. S. Distribution of HCV genotypes among blood donors, patients with chronic liver disease, hepatocellular carcinoma, and patients on maintenance hemodialysis in Korea. J Med Virol. 1996 May;49(1):55–60. doi: 10.1002/(SICI)1096-9071(199605)49:1<55::AID-JMV9>3.0.CO;2-J. [DOI] [PubMed] [Google Scholar]
  7. Mahaney K., Tedeschi V., Maertens G., Di Bisceglie A. M., Vergalla J., Hoofnagle J. H., Sallie R. Genotypic analysis of hepatitis C virus in American patients. Hepatology. 1994 Dec;20(6):1405–1411. doi: 10.1002/hep.1840200605. [DOI] [PubMed] [Google Scholar]
  8. Offit P. A., Clark H. F., Stroop W. G., Twist E. M., Plotkin S. A. The cultivation of human rotavirus, strain 'Wa', to high titer in cell culture and characterization of the viral structural polypeptides. J Virol Methods. 1983 Jul;7(1):29–40. doi: 10.1016/0166-0934(83)90020-4. [DOI] [PubMed] [Google Scholar]
  9. Okuda K., Hayashi H., Yokozeki K., Irie Y. Destruction of hepatitis C virus particles by haemodialysis. Lancet. 1996 Mar 30;347(9005):909–910. doi: 10.1016/s0140-6736(96)91397-5. [DOI] [PubMed] [Google Scholar]
  10. Oubiña J. R., Quarleri J. F., Rudzinski M., Parks C., Badía I., González Cappa S. M. Genomic characterization of hepatitis C virus isolates from Argentina. J Med Virol. 1995 Sep;47(1):97–104. doi: 10.1002/jmv.1890470118. [DOI] [PubMed] [Google Scholar]
  11. Pol S., Thiers V., Nousbaum J. B., Legendre C., Berthelot P., Kreis H., Brechot C. The changing relative prevalence of hepatitis C virus genotypes: evidence in hemodialyzed patients and kidney recipients. Gastroenterology. 1995 Feb;108(2):581–583. doi: 10.1016/0016-5085(95)90088-8. [DOI] [PubMed] [Google Scholar]
  12. Silini E., Bono F., Cividini A., Cerino A., Bruno S., Rossi S., Belloni G., Brugnetti B., Civardi E., Salvaneschi L. Differential distribution of hepatitis C virus genotypes in patients with and without liver function abnormalities. Hepatology. 1995 Feb;21(2):285–290. [PubMed] [Google Scholar]
  13. Smith D. B., Mellor J., Jarvis L. M., Davidson F., Kolberg J., Urdea M., Yap P. L., Simmonds P. Variation of the hepatitis C virus 5' non-coding region: implications for secondary structure, virus detection and typing. The International HCV Collaborative Study Group. J Gen Virol. 1995 Jul;76(Pt 7):1749–1761. doi: 10.1099/0022-1317-76-7-1749. [DOI] [PubMed] [Google Scholar]
  14. Stuyver L., Wyseur A., van Arnhem W., Hernandez F., Maertens G. Second-generation line probe assay for hepatitis C virus genotyping. J Clin Microbiol. 1996 Sep;34(9):2259–2266. doi: 10.1128/jcm.34.9.2259-2266.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Widell A., Shev S., Månsson S., Zhang Y. Y., Foberg U., Norkrans G., Frydén A., Weiland O., Kurkus J., Nordenfelt E. Genotyping of hepatitis C virus isolates by a modified polymerase chain reaction assay using type specific primers: epidemiological applications. J Med Virol. 1994 Nov;44(3):272–279. doi: 10.1002/jmv.1890440311. [DOI] [PubMed] [Google Scholar]
  16. Wu J. S., Lee H. F., Hsiau H. L., Lu H. Y., Chou W. H., Lu C. F., Chen H. Y., Lee F. N., Chen P. Y., Tam K. M. Genotype distribution of hepatitis C virus infection in Taiwan. J Med Virol. 1994 Sep;44(1):74–79. doi: 10.1002/jmv.1890440114. [DOI] [PubMed] [Google Scholar]

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