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. 1997 Jul;35(7):1887–1890. doi: 10.1128/jcm.35.7.1887-1890.1997

Monoclonal antibodies capable of distinguishing epizootic from enzootic varieties of subtype 1 Venezuelan equine encephalitis viruses in a rapid indirect immunofluorescence assay.

J T Roehrig 1, R A Bolin 1
PMCID: PMC229865  PMID: 9196217

Abstract

We used previously characterized murine monoclonal antibodies to develop a panel useful in subtyping Venezuelan equine encephalitis (VEE) viruses by an indirect fluorescent antibody assay. This panel worked well with either prototype VEE viruses or a series of more recent VEE virus isolates. The panel is particularly useful for rapidly differentiating VEE viruses with epidemic-epizootic potential from other endemic varieties of this virus. Using this panel, we identified an antigenic variant of prototype VEE subtype 1E virus currently present in Mexico. This antigenic change in the E2 glycoprotein was confirmed by enzyme-linked immunosorbent assay. Because VEE virus virulence has been associated in part with the E2 glycoprotein, this observed antigenic change in the 1E virus E2 glycoprotein may explain the apparent equine virulence of this unusual VEE 1E virus.

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Selected References

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  1. Hunt A. R., Roehrig J. T. Biochemical and biological characteristics of epitopes on the E1 glycoprotein of western equine encephalitis virus. Virology. 1985 Apr 30;142(2):334–346. doi: 10.1016/0042-6822(85)90342-3. [DOI] [PubMed] [Google Scholar]
  2. Johnson B. J., Brubaker J. R., Roehrig J. T., Trent D. W. Variants of Venezuelan equine encephalitis virus that resist neutralization define a domain of the E2 glycoprotein. Virology. 1990 Aug;177(2):676–683. doi: 10.1016/0042-6822(90)90533-w. [DOI] [PubMed] [Google Scholar]
  3. Kinney R. M., Chang G. J., Tsuchiya K. R., Sneider J. M., Roehrig J. T., Woodward T. M., Trent D. W. Attenuation of Venezuelan equine encephalitis virus strain TC-83 is encoded by the 5'-noncoding region and the E2 envelope glycoprotein. J Virol. 1993 Mar;67(3):1269–1277. doi: 10.1128/jvi.67.3.1269-1277.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kinney R. M., Tsuchiya K. R., Sneider J. M., Trent D. W. Genetic evidence that epizootic Venezuelan equine encephalitis (VEE) viruses may have evolved from enzootic VEE subtype I-D virus. Virology. 1992 Dec;191(2):569–580. doi: 10.1016/0042-6822(92)90232-e. [DOI] [PubMed] [Google Scholar]
  5. Kinney R. M., Tsuchiya K. R., Sneider J. M., Trent D. W. Molecular evidence for the origin of the widespread Venezuelan equine encephalitis epizootic of 1969 to 1972. J Gen Virol. 1992 Dec;73(Pt 12):3301–3305. doi: 10.1099/0022-1317-73-12-3301. [DOI] [PubMed] [Google Scholar]
  6. Rico-Hesse R., Roehrig J. T., Dickerman R. W. Monoclonal antibodies define antigenic variation in the ID variety of Venezuelan equine encephalitis virus. Am J Trop Med Hyg. 1988 Jan;38(1):187–194. doi: 10.4269/ajtmh.1988.38.187. [DOI] [PubMed] [Google Scholar]
  7. Rico-Hesse R., Weaver S. C., de Siger J., Medina G., Salas R. A. Emergence of a new epidemic/epizootic Venezuelan equine encephalitis virus in South America. Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5278–5281. doi: 10.1073/pnas.92.12.5278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Roehrig J. T., Bolin R. A., Hunt A. R., Woodward T. M. Use of a new synthetic-peptide-derived monoclonal antibody to differentiate between vaccine and wild-type Venezuelan equine encephalomyelitis viruses. J Clin Microbiol. 1991 Mar;29(3):630–631. doi: 10.1128/jcm.29.3.630-631.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Roehrig J. T., Day J. W., Kinney R. M. Antigenic analysis of the surface glycoproteins of a Venezuelan equine encephalomyelitis virus (TC-83) using monoclonal antibodies. Virology. 1982 Apr 30;118(2):269–278. doi: 10.1016/0042-6822(82)90346-4. [DOI] [PubMed] [Google Scholar]
  10. Roehrig J. T., Hunt A. R., Chang G. J., Sheik B., Bolin R. A., Tsai T. F., Trent D. W. Identification of monoclonal antibodies capable of differentiating antigenic varieties of eastern equine encephalitis viruses. Am J Trop Med Hyg. 1990 Apr;42(4):394–398. doi: 10.4269/ajtmh.1990.42.394. [DOI] [PubMed] [Google Scholar]
  11. Roehrig J. T., Mathews J. H. The neutralization site on the E2 glycoprotein of Venezuelan equine encephalomyelitis (TC-83) virus is composed of multiple conformationally stable epitopes. Virology. 1985 Apr 30;142(2):347–356. doi: 10.1016/0042-6822(85)90343-5. [DOI] [PubMed] [Google Scholar]
  12. Roehrig J. T., Mathews J. H., Trent D. W. Identification of epitopes on the E glycoprotein of Saint Louis encephalitis virus using monoclonal antibodies. Virology. 1983 Jul 15;128(1):118–126. doi: 10.1016/0042-6822(83)90323-9. [DOI] [PubMed] [Google Scholar]
  13. Sneider J. M., Kinney R. M., Tsuchiya K. R., Trent D. W. Molecular evidence that epizootic Venezuelan equine encephalitis (VEE) I-AB viruses are not evolutionary derivatives of enzootic VEE subtype I-E or II viruses. J Gen Virol. 1993 Mar;74(Pt 3):519–523. doi: 10.1099/0022-1317-74-3-519. [DOI] [PubMed] [Google Scholar]
  14. Weaver S. C., Bellew L. A., Rico-Hesse R. Phylogenetic analysis of alphaviruses in the Venezuelan equine encephalitis complex and identification of the source of epizootic viruses. Virology. 1992 Nov;191(1):282–290. doi: 10.1016/0042-6822(92)90190-z. [DOI] [PubMed] [Google Scholar]

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