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. 1997 Aug;35(8):1988–1995. doi: 10.1128/jcm.35.8.1988-1995.1997

Phylogenetic placement and characterization of a new alpha-2 proteobacterium isolated from a patient with sepsis.

G Blomqvist 1, L Wesslén 1, C Påhlson 1, E Hjelm 1, B Pettersson 1, T Nikkilä 1, U Allard 1, O Svensson 1, M Uhlén 1, B Morein 1, G Friman 1
PMCID: PMC229889  PMID: 9230368

Abstract

An alpha-2 proteobacterium, previously unknown as determined by its phylogenetic characteristics and the DNA sequence of its 16S rRNA gene, was isolated from a patient who presented an unusual clinical picture, including high remitting fever and multiorgan involvement. The bacterium was detected in multiple plasma samples, obtained during the acute phase of the disease, after cocultivation in cell culture media. Electron microscopy of the organism showed a three-layer laminar cell wall and electron-dense granules within the cytoplasm, as well as a polar flagellum. By means of PCR followed by sequencing of amplified 16S ribosomal DNA fragments, the bacterium was found to differ from all species for which ribosomal sequence information is available. It is here provisionally named the Rasbo bacterium. At a subsequent relapse, the bacterium was identified in pericardial fluid both by PCR/sequencing and by direct electron microscopy. At a second relapse, it was again cultured from plasma. After in vitro adaptation to solid media, the MICs of various antibiotics could be determined. A transient immunoglobulin M (IgM) but no IgG response to the bacterium was found by an indirect immunofluorescence test, as well as by an immobilization test during the acute phase of the disease.

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Selected References

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  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. doi: 10.1016/S0022-2836(05)80360-2. [DOI] [PubMed] [Google Scholar]
  2. Birkness K. A., George V. G., White E. H., Stephens D. S., Quinn F. D. Intracellular growth of Afipia felis, a putative etiologic agent of cat scratch disease. Infect Immun. 1992 Jun;60(6):2281–2287. doi: 10.1128/iai.60.6.2281-2287.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brenner D. J., O'Connor S. P., Winkler H. H., Steigerwalt A. G. Proposals to unify the genera Bartonella and Rochalimaea, with descriptions of Bartonella quintana comb. nov., Bartonella vinsonii comb. nov., Bartonella henselae comb. nov., and Bartonella elizabethae comb. nov., and to remove the family Bartonellaceae from the order Rickettsiales. Int J Syst Bacteriol. 1993 Oct;43(4):777–786. doi: 10.1099/00207713-43-4-777. [DOI] [PubMed] [Google Scholar]
  4. Claflin A. J., Smithies O., Meyer R. K. Antibody responses in bursa-deficient chickens. J Immunol. 1966 Nov;97(5):693–699. [PubMed] [Google Scholar]
  5. Cles L. D., Stamm W. E. Use of HL cells for improved isolation and passage of Chlamydia pneumoniae. J Clin Microbiol. 1990 May;28(5):938–940. doi: 10.1128/jcm.28.5.938-940.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Daly J. S., Worthington M. G., Brenner D. J., Moss C. W., Hollis D. G., Weyant R. S., Steigerwalt A. G., Weaver R. E., Daneshvar M. I., O'Connor S. P. Rochalimaea elizabethae sp. nov. isolated from a patient with endocarditis. J Clin Microbiol. 1993 Apr;31(4):872–881. doi: 10.1128/jcm.31.4.872-881.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Drancourt M., Mainardi J. L., Brouqui P., Vandenesch F., Carta A., Lehnert F., Etienne J., Goldstein F., Acar J., Raoult D. Bartonella (Rochalimaea) quintana endocarditis in three homeless men. N Engl J Med. 1995 Feb 16;332(7):419–423. doi: 10.1056/NEJM199502163320702. [DOI] [PubMed] [Google Scholar]
  8. Drancourt M., Moal V., Brunet P., Dussol B., Berland Y., Raoult D. Bartonella (Rochalimaea) quintana infection in a seronegative hemodialyzed patient. J Clin Microbiol. 1996 May;34(5):1158–1160. doi: 10.1128/jcm.34.5.1158-1160.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. English C. K., Wear D. J., Margileth A. M., Lissner C. R., Walsh G. P. Cat-scratch disease. Isolation and culture of the bacterial agent. JAMA. 1988 Mar 4;259(9):1347–1352. doi: 10.1001/jama.259.9.1347. [DOI] [PubMed] [Google Scholar]
  10. Hultman T., Bergh S., Moks T., Uhlén M. Bidirectional solid-phase sequencing of in vitro-amplified plasmid DNA. Biotechniques. 1991 Jan;10(1):84–93. [PubMed] [Google Scholar]
  11. Hultman T., Ståhl S., Hornes E., Uhlén M. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res. 1989 Jul 11;17(13):4937–4946. doi: 10.1093/nar/17.13.4937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Maidak B. L., Olsen G. J., Larsen N., Overbeek R., McCaughey M. J., Woese C. R. The Ribosomal Database Project (RDP). Nucleic Acids Res. 1996 Jan 1;24(1):82–85. doi: 10.1093/nar/24.1.82. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Olsen G. J., Woese C. R. Ribosomal RNA: a key to phylogeny. FASEB J. 1993 Jan;7(1):113–123. doi: 10.1096/fasebj.7.1.8422957. [DOI] [PubMed] [Google Scholar]
  14. Pettersson B., Johansson K. E., Uhlén M. Sequence analysis of 16S rRNA from mycoplasmas by direct solid-phase DNA sequencing. Appl Environ Microbiol. 1994 Jul;60(7):2456–2461. doi: 10.1128/aem.60.7.2456-2461.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pettersson B., Leitner T., Ronaghi M., Bölske G., Uhlen M., Johansson K. E. Phylogeny of the Mycoplasma mycoides cluster as determined by sequence analysis of the 16S rRNA genes from the two rRNA operons. J Bacteriol. 1996 Jul;178(14):4131–4142. doi: 10.1128/jb.178.14.4131-4142.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pettersson B., Uhlén M., Johansson K. E. Phylogeny of some mycoplasmas from ruminants based on 16S rRNA sequences and definition of a new cluster within the hominis group. Int J Syst Bacteriol. 1996 Oct;46(4):1093–1098. doi: 10.1099/00207713-46-4-1093. [DOI] [PubMed] [Google Scholar]
  17. Regnery R. L., Anderson B. E., Clarridge J. E., 3rd, Rodriguez-Barradas M. C., Jones D. C., Carr J. H. Characterization of a novel Rochalimaea species, R. henselae sp. nov., isolated from blood of a febrile, human immunodeficiency virus-positive patient. J Clin Microbiol. 1992 Feb;30(2):265–274. doi: 10.1128/jcm.30.2.265-274.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Relman D. A., Loutit J. S., Schmidt T. M., Falkow S., Tompkins L. S. The agent of bacillary angiomatosis. An approach to the identification of uncultured pathogens. N Engl J Med. 1990 Dec 6;323(23):1573–1580. doi: 10.1056/NEJM199012063232301. [DOI] [PubMed] [Google Scholar]
  19. Ripa K. T., Mårdh P. A. Cultivation of Chlamydia trachomatis in cycloheximide-treated mccoy cells. J Clin Microbiol. 1977 Oct;6(4):328–331. doi: 10.1128/jcm.6.4.328-331.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Saitou N., Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987 Jul;4(4):406–425. doi: 10.1093/oxfordjournals.molbev.a040454. [DOI] [PubMed] [Google Scholar]
  21. Tully J. G., Whitcomb R. F., Clark H. F., Williamson D. L. Pathogenic mycoplasmas: cultivation and vertebrate pathogenicity of a new spiroplasma. Science. 1977 Mar 4;195(4281):892–894. doi: 10.1126/science.841314. [DOI] [PubMed] [Google Scholar]
  22. Wahlberg J., Holmberg A., Bergh S., Hultman T., Uhlén M. Automated magnetic preparation of DNA templates for solid phase sequencing. Electrophoresis. 1992 Aug;13(8):547–551. doi: 10.1002/elps.11501301112. [DOI] [PubMed] [Google Scholar]
  23. Wang S. P., Grayston J. T. Immunologic relationship between genital TRIC, lymphogranuloma venereum, and related organisms in a new microtiter indirect immunofluorescence test. Am J Ophthalmol. 1970 Sep;70(3):367–374. doi: 10.1016/0002-9394(70)90096-6. [DOI] [PubMed] [Google Scholar]
  24. Wilkinson H. W., Fikes B. J., Cruce D. D. Indirect immunofluorescence test for serodiagnosis of Legionnaires disease: evidence for serogroup diversity of Legionnaires disease bacterial antigens and for multiple specificity of human antibodies. J Clin Microbiol. 1979 Mar;9(3):379–383. doi: 10.1128/jcm.9.3.379-383.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Woese C. R. Bacterial evolution. Microbiol Rev. 1987 Jun;51(2):221–271. doi: 10.1128/mr.51.2.221-271.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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