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. 1997 Aug;35(8):2188–2189. doi: 10.1128/jcm.35.8.2188-2189.1997

The practical use of PCR for rapid detection of methicillin resistance among staphylococcal clinical isolates from Turkish hospitals.

S Kocagöz, S Unal
PMCID: PMC229937  PMID: 9230416

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Selected References

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  1. Alfa M. J., Ongsansoy E., Hoban S., Mathew T., Greenaway C. Methicillin-resistant Staphylococcus aureus. Optimization of the agar screen plate method. Diagn Microbiol Infect Dis. 1994 Feb;18(2):121–124. doi: 10.1016/0732-8893(94)90077-9. [DOI] [PubMed] [Google Scholar]
  2. Chambers H. F. Coagulase-negative staphylococci resistant to beta-lactam antibiotics in vivo produce penicillin-binding protein 2a. Antimicrob Agents Chemother. 1987 Dec;31(12):1919–1924. doi: 10.1128/aac.31.12.1919. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chambers H. F., Hackbarth C. J. Effect of NaCl and nafcillin on penicillin-binding protein 2a and heterogeneous expression of methicillin resistance in Staphylococcus aureus. Antimicrob Agents Chemother. 1987 Dec;31(12):1982–1988. doi: 10.1128/aac.31.12.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gerberding J. L., Miick C., Liu H. H., Chambers H. F. Comparison of conventional susceptibility tests with direct detection of penicillin-binding protein 2a in borderline oxacillin-resistant strains of Staphylococcus aureus. Antimicrob Agents Chemother. 1991 Dec;35(12):2574–2579. doi: 10.1128/aac.35.12.2574. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hackbarth C. J., Chambers H. F. Methicillin-resistant staphylococci: genetics and mechanisms of resistance. Antimicrob Agents Chemother. 1989 Jul;33(7):991–994. doi: 10.1128/aac.33.7.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hartman B. J., Tomasz A. Low-affinity penicillin-binding protein associated with beta-lactam resistance in Staphylococcus aureus. J Bacteriol. 1984 May;158(2):513–516. doi: 10.1128/jb.158.2.513-516.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McDonald C. L., Maher W. E., Fass R. J. Revised interpretation of oxacillin MICs for Staphylococcus epidermidis based on mecA detection. Antimicrob Agents Chemother. 1995 Apr;39(4):982–984. doi: 10.1128/aac.39.4.982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Murakami K., Minamide W., Wada K., Nakamura E., Teraoka H., Watanabe S. Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction. J Clin Microbiol. 1991 Oct;29(10):2240–2244. doi: 10.1128/jcm.29.10.2240-2244.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Unal S., Hoskins J., Flokowitsch J. E., Wu C. Y., Preston D. A., Skatrud P. L. Detection of methicillin-resistant staphylococci by using the polymerase chain reaction. J Clin Microbiol. 1992 Jul;30(7):1685–1691. doi: 10.1128/jcm.30.7.1685-1691.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Unal S., Werner K., DeGirolami P., Barsanti F., Eliopoulos G. Comparison of tests for detection of methicillin-resistant Staphylococcus aureus in a clinical microbiology laboratory. Antimicrob Agents Chemother. 1994 Feb;38(2):345–347. doi: 10.1128/aac.38.2.345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Vannuffel P., Gigi J., Ezzedine H., Vandercam B., Delmee M., Wauters G., Gala J. L. Specific detection of methicillin-resistant Staphylococcus species by multiplex PCR. J Clin Microbiol. 1995 Nov;33(11):2864–2867. doi: 10.1128/jcm.33.11.2864-2867.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. York M. K., Gibbs L., Chehab F., Brooks G. F. Comparison of PCR detection of mecA with standard susceptibility testing methods to determine methicillin resistance in coagulase-negative staphylococci. J Clin Microbiol. 1996 Feb;34(2):249–253. doi: 10.1128/jcm.34.2.249-253.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]

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