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. 1997 Sep;35(9):2256–2261. doi: 10.1128/jcm.35.9.2256-2261.1997

Prevalence of Bartonella species in domestic cats in The Netherlands.

A M Bergmans 1, C M de Jong 1, G van Amerongen 1, C S Schot 1, L M Schouls 1
PMCID: PMC229949  PMID: 9276397

Abstract

Cats have been shown to provide the only known reservoir of Bartonella henselae, the causative agent of cat scratch disease. To determine the prevalence of Bartonella bacteremia and antibodies in Dutch cats, blood samples from 113 cats from shelters (sheltered cats), 50 pet cats, and 25 specific-pathogen-free (SPF) cats were analyzed. Culture and subsequent PCR-restriction fragment length polymorphism (RFLP) analysis of the 16S-23S rRNA intergenic region and 16S rRNA gene PCR-hybridization assays revealed a prevalence of Bartonella bacteremia in 22% of the sheltered cats and showed no bacteremia in the SPF cats. Three spacer RFLP types were found: types A, B, and G, with type B being predominant over types A and G. An important finding was the existence of mixtures of different Bartonella species. Bartonella DNA was detected in 7 of 27 DNA extracts from fleas combed from the sheltered cats (26%). Seropositivity was 50% for sheltered cats and 56% for pet cats, as determined by a B. henselae enzyme-linked immunoassay.

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Selected References

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  1. Anderson B., Sims K., Regnery R., Robinson L., Schmidt M. J., Goral S., Hager C., Edwards K. Detection of Rochalimaea henselae DNA in specimens from cat scratch disease patients by PCR. J Clin Microbiol. 1994 Apr;32(4):942–948. doi: 10.1128/jcm.32.4.942-948.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Angulo F. J., Glaser C. A., Juranek D. D., Lappin M. R., Regnery R. L. Caring for pets of immunocompromised persons. J Am Vet Med Assoc. 1994 Dec 15;205(12):1711–1718. [PubMed] [Google Scholar]
  3. Barka N. E., Hadfield T., Patnaik M., Schwartzman W. A., Peter J. B. EIA for detection of Rochalimaea henselae-reactive IgG, IgM, and IgA antibodies in patients with suspected cat-scratch disease. J Infect Dis. 1993 Jun;167(6):1503–1504. doi: 10.1093/infdis/167.6.1503. [DOI] [PubMed] [Google Scholar]
  4. Bergmans A. M., Groothedde J. W., Schellekens J. F., van Embden J. D., Ossewaarde J. M., Schouls L. M. Etiology of cat scratch disease: comparison of polymerase chain reaction detection of Bartonella (formerly Rochalimaea) and Afipia felis DNA with serology and skin tests. J Infect Dis. 1995 Apr;171(4):916–923. doi: 10.1093/infdis/171.4.916. [DOI] [PubMed] [Google Scholar]
  5. Bergmans A. M., Schellekens J. F., van Embden J. D., Schouls L. M. Predominance of two Bartonella henselae variants among cat-scratch disease patients in the Netherlands. J Clin Microbiol. 1996 Feb;34(2):254–260. doi: 10.1128/jcm.34.2.254-260.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Breitschwerdt E. B., Kordick D. L. Bartonellosis. J Am Vet Med Assoc. 1995 Jun 15;206(12):1928–1931. [PubMed] [Google Scholar]
  7. Carithers H. A. Cat-scratch disease. An overview based on a study of 1,200 patients. Am J Dis Child. 1985 Nov;139(11):1124–1133. doi: 10.1001/archpedi.1985.02140130062031. [DOI] [PubMed] [Google Scholar]
  8. Childs J. E., Olson J. G., Wolf A., Cohen N., Fakile Y., Rooney J. A., Bacellar F., Regnery R. L. Prevalence of antibodies to Rochalimaea species (cat-scratch disease agent) in cats. Vet Rec. 1995 May 20;136(20):519–520. doi: 10.1136/vr.136.20.519. [DOI] [PubMed] [Google Scholar]
  9. Chomel B. B., Abbott R. C., Kasten R. W., Floyd-Hawkins K. A., Kass P. H., Glaser C. A., Pedersen N. C., Koehler J. E. Bartonella henselae prevalence in domestic cats in California: risk factors and association between bacteremia and antibody titers. J Clin Microbiol. 1995 Sep;33(9):2445–2450. doi: 10.1128/jcm.33.9.2445-2450.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Chomel B. B., Kasten R. W., Floyd-Hawkins K., Chi B., Yamamoto K., Roberts-Wilson J., Gurfield A. N., Abbott R. C., Pedersen N. C., Koehler J. E. Experimental transmission of Bartonella henselae by the cat flea. J Clin Microbiol. 1996 Aug;34(8):1952–1956. doi: 10.1128/jcm.34.8.1952-1956.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Clarridge J. E., 3rd, Raich T. J., Pirwani D., Simon B., Tsai L., Rodriguez-Barradas M. C., Regnery R., Zollo A., Jones D. C., Rambo C. Strategy to detect and identify Bartonella species in routine clinical laboratory yields Bartonella henselae from human immunodeficiency virus-positive patient and unique Bartonella strain from his cat. J Clin Microbiol. 1995 Aug;33(8):2107–2113. doi: 10.1128/jcm.33.8.2107-2113.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Dolan M. J., Wong M. T., Regnery R. L., Jorgensen J. H., Garcia M., Peters J., Drehner D. Syndrome of Rochalimaea henselae adenitis suggesting cat scratch disease. Ann Intern Med. 1993 Mar 1;118(5):331–336. doi: 10.7326/0003-4819-118-5-199303010-00002. [DOI] [PubMed] [Google Scholar]
  13. Greene C. E., McDermott M., Jameson P. H., Atkins C. L., Marks A. M. Bartonella henselae infection in cats: evaluation during primary infection, treatment, and rechallenge infection. J Clin Microbiol. 1996 Jul;34(7):1682–1685. doi: 10.1128/jcm.34.7.1682-1685.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hadfield T. L., Warren R., Kass M., Brun E., Levy C. Endocarditis caused by Rochalimaea henselae. Hum Pathol. 1993 Oct;24(10):1140–1141. doi: 10.1016/0046-8177(93)90196-n. [DOI] [PubMed] [Google Scholar]
  15. Jameson P., Greene C., Regnery R., Dryden M., Marks A., Brown J., Cooper J., Glaus B., Greene R. Prevalence of Bartonella henselae antibodies in pet cats throughout regions of North America. J Infect Dis. 1995 Oct;172(4):1145–1149. doi: 10.1093/infdis/172.4.1145. [DOI] [PubMed] [Google Scholar]
  16. Koehler J. E., Glaser C. A., Tappero J. W. Rochalimaea henselae infection. A new zoonosis with the domestic cat as reservoir. JAMA. 1994 Feb 16;271(7):531–535. doi: 10.1001/jama.271.7.531. [DOI] [PubMed] [Google Scholar]
  17. Koehler J. E., Quinn F. D., Berger T. G., LeBoit P. E., Tappero J. W. Isolation of Rochalimaea species from cutaneous and osseous lesions of bacillary angiomatosis. N Engl J Med. 1992 Dec 3;327(23):1625–1631. doi: 10.1056/NEJM199212033272303. [DOI] [PubMed] [Google Scholar]
  18. Kordick D. L., Wilson K. H., Sexton D. J., Hadfield T. L., Berkhoff H. A., Breitschwerdt E. B. Prolonged Bartonella bacteremia in cats associated with cat-scratch disease patients. J Clin Microbiol. 1995 Dec;33(12):3245–3251. doi: 10.1128/jcm.33.12.3245-3251.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Matar G. M., Swaminathan B., Hunter S. B., Slater L. N., Welch D. F. Polymerase chain reaction-based restriction fragment length polymorphism analysis of a fragment of the ribosomal operon from Rochalimaea species for subtyping. J Clin Microbiol. 1993 Jul;31(7):1730–1734. doi: 10.1128/jcm.31.7.1730-1734.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pearson W. R., Lipman D. J. Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444–2448. doi: 10.1073/pnas.85.8.2444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Regnery R. L., Anderson B. E., Clarridge J. E., 3rd, Rodriguez-Barradas M. C., Jones D. C., Carr J. H. Characterization of a novel Rochalimaea species, R. henselae sp. nov., isolated from blood of a febrile, human immunodeficiency virus-positive patient. J Clin Microbiol. 1992 Feb;30(2):265–274. doi: 10.1128/jcm.30.2.265-274.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Regnery R. L., Olson J. G., Perkins B. A., Bibb W. Serological response to "Rochalimaea henselae" antigen in suspected cat-scratch disease. Lancet. 1992 Jun 13;339(8807):1443–1445. doi: 10.1016/0140-6736(92)92032-b. [DOI] [PubMed] [Google Scholar]
  23. Regnery R., Martin M., Olson J. Naturally occurring "Rochalimaea henselae" infection in domestic cat. Lancet. 1992 Aug 29;340(8818):557–558. doi: 10.1016/0140-6736(92)91760-6. [DOI] [PubMed] [Google Scholar]
  24. Relman D. A., Loutit J. S., Schmidt T. M., Falkow S., Tompkins L. S. The agent of bacillary angiomatosis. An approach to the identification of uncultured pathogens. N Engl J Med. 1990 Dec 6;323(23):1573–1580. doi: 10.1056/NEJM199012063232301. [DOI] [PubMed] [Google Scholar]
  25. Slater L. N., Welch D. F., Hensel D., Coody D. W. A newly recognized fastidious gram-negative pathogen as a cause of fever and bacteremia. N Engl J Med. 1990 Dec 6;323(23):1587–1593. doi: 10.1056/NEJM199012063232303. [DOI] [PubMed] [Google Scholar]
  26. Spach D. H., Callis K. P., Paauw D. S., Houze Y. B., Schoenknecht F. D., Welch D. F., Rosen H., Brenner D. J. Endocarditis caused by Rochalimaea quintana in a patient infected with human immunodeficiency virus. J Clin Microbiol. 1993 Mar;31(3):692–694. doi: 10.1128/jcm.31.3.692-694.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Tappero J. W., Mohle-Boetani J., Koehler J. E., Swaminathan B., Berger T. G., LeBoit P. E., Smith L. L., Wenger J. D., Pinner R. W., Kemper C. A. The epidemiology of bacillary angiomatosis and bacillary peliosis. JAMA. 1993 Feb 10;269(6):770–775. [PubMed] [Google Scholar]
  28. Ueno H., Muramatsu Y., Chomel B. B., Hohdatsu T., Koyama H., Morita C. Seroepidemiological survey of Bartonella (Rochalimaea) henselae in domestic cats in Japan. Microbiol Immunol. 1995;39(5):339–341. doi: 10.1111/j.1348-0421.1995.tb02210.x. [DOI] [PubMed] [Google Scholar]
  29. Zangwill K. M., Hamilton D. H., Perkins B. A., Regnery R. L., Plikaytis B. D., Hadler J. L., Cartter M. L., Wenger J. D. Cat scratch disease in Connecticut. Epidemiology, risk factors, and evaluation of a new diagnostic test. N Engl J Med. 1993 Jul 1;329(1):8–13. doi: 10.1056/NEJM199307013290102. [DOI] [PubMed] [Google Scholar]

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