Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1997 Sep;35(9):2393–2397. doi: 10.1128/jcm.35.9.2393-2397.1997

DNA fingerprinting of Mycobacterium tuberculosis strains from patients with pulmonary tuberculosis in Honduras.

L Pineda-Garcia 1, A Ferrera 1, S E Hoffner 1
PMCID: PMC229974  PMID: 9276422

Abstract

Mycobacterium tuberculosis isolates from 84 patients with pulmonary tuberculosis in Honduras were characterized by restriction fragment length polymorphism analysis. Seventy-three different IS6110 patterns were found; 63 of these were unique and 10 were shared by two to three strains each. Thus, no ongoing spread of any specific clone of bacteria could be demonstrated.

Full Text

The Full Text of this article is available as a PDF (326.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alland D., Kalkut G. E., Moss A. R., McAdam R. A., Hahn J. A., Bosworth W., Drucker E., Bloom B. R. Transmission of tuberculosis in New York City. An analysis by DNA fingerprinting and conventional epidemiologic methods. N Engl J Med. 1994 Jun 16;330(24):1710–1716. doi: 10.1056/NEJM199406163302403. [DOI] [PubMed] [Google Scholar]
  2. Bloom B. R., Murray C. J. Tuberculosis: commentary on a reemergent killer. Science. 1992 Aug 21;257(5073):1055–1064. doi: 10.1126/science.257.5073.1055. [DOI] [PubMed] [Google Scholar]
  3. Cave M. D., Eisenach K. D., McDermott P. F., Bates J. H., Crawford J. T. IS6110: conservation of sequence in the Mycobacterium tuberculosis complex and its utilization in DNA fingerprinting. Mol Cell Probes. 1991 Feb;5(1):73–80. doi: 10.1016/0890-8508(91)90040-q. [DOI] [PubMed] [Google Scholar]
  4. Chevrel-Dellagi D., Abderrahman A., Haltiti R., Koubaji H., Gicquel B., Dellagi K. Large-scale DNA fingerprinting of Mycobacterium tuberculosis strains as a tool for epidemiological studies of tuberculosis. J Clin Microbiol. 1993 Sep;31(9):2446–2450. doi: 10.1128/jcm.31.9.2446-2450.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Daley C. L., Small P. M., Schecter G. F., Schoolnik G. K., McAdam R. A., Jacobs W. R., Jr, Hopewell P. C. An outbreak of tuberculosis with accelerated progression among persons infected with the human immunodeficiency virus. An analysis using restriction-fragment-length polymorphisms. N Engl J Med. 1992 Jan 23;326(4):231–235. doi: 10.1056/NEJM199201233260404. [DOI] [PubMed] [Google Scholar]
  6. Friedman C. R., Stoeckle M. Y., Kreiswirth B. N., Johnson W. D., Jr, Manoach S. M., Berger J., Sathianathan K., Hafner A., Riley L. W. Transmission of multidrug-resistant tuberculosis in a large urban setting. Am J Respir Crit Care Med. 1995 Jul;152(1):355–359. doi: 10.1164/ajrccm.152.1.7599845. [DOI] [PubMed] [Google Scholar]
  7. Gómez Marín J. E., Rigouts L., Villegas Londoño L. E., Portaels F. Restriction fragment length polymorphism (RFLP) analysis and tuberculosis epidemiology. Bull Pan Am Health Organ. 1995 Sep;29(3):226–236. [PubMed] [Google Scholar]
  8. Hermans P. W., Messadi F., Guebrexabher H., van Soolingen D., de Haas P. E., Heersma H., de Neeling H., Ayoub A., Portaels F., Frommel D. Analysis of the population structure of Mycobacterium tuberculosis in Ethiopia, Tunisia, and The Netherlands: usefulness of DNA typing for global tuberculosis epidemiology. J Infect Dis. 1995 Jun;171(6):1504–1513. doi: 10.1093/infdis/171.6.1504. [DOI] [PubMed] [Google Scholar]
  9. Hermans P. W., van Soolingen D., Dale J. W., Schuitema A. R., McAdam R. A., Catty D., van Embden J. D. Insertion element IS986 from Mycobacterium tuberculosis: a useful tool for diagnosis and epidemiology of tuberculosis. J Clin Microbiol. 1990 Sep;28(9):2051–2058. doi: 10.1128/jcm.28.9.2051-2058.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mazurek G. H., Cave M. D., Eisenach K. D., Wallace R. J., Jr, Bates J. H., Crawford J. T. Chromosomal DNA fingerprint patterns produced with IS6110 as strain-specific markers for epidemiologic study of tuberculosis. J Clin Microbiol. 1991 Sep;29(9):2030–2033. doi: 10.1128/jcm.29.9.2030-2033.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Poulet S., Cole S. T. Repeated DNA sequences in mycobacteria. Arch Microbiol. 1995 Feb;163(2):79–86. doi: 10.1007/BF00381780. [DOI] [PubMed] [Google Scholar]
  12. Small P. M., Hopewell P. C., Singh S. P., Paz A., Parsonnet J., Ruston D. C., Schecter G. F., Daley C. L., Schoolnik G. K. The epidemiology of tuberculosis in San Francisco. A population-based study using conventional and molecular methods. N Engl J Med. 1994 Jun 16;330(24):1703–1709. doi: 10.1056/NEJM199406163302402. [DOI] [PubMed] [Google Scholar]
  13. Sola C., Horgen L., Goh K. S., Rastogi N. Molecular fingerprinting of Mycobacterium tuberculosis on a Caribbean island with IS6110 and DRr probes. J Clin Microbiol. 1997 Apr;35(4):843–846. doi: 10.1128/jcm.35.4.843-846.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Thierry D., Cave M. D., Eisenach K. D., Crawford J. T., Bates J. H., Gicquel B., Guesdon J. L. IS6110, an IS-like element of Mycobacterium tuberculosis complex. Nucleic Acids Res. 1990 Jan 11;18(1):188–188. doi: 10.1093/nar/18.1.188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Yang Z. H., de Haas P. E., Wachmann C. H., van Soolingen D., van Embden J. D., Andersen A. B. Molecular epidemiology of tuberculosis in Denmark in 1992. J Clin Microbiol. 1995 Aug;33(8):2077–2081. doi: 10.1128/jcm.33.8.2077-2081.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. van Embden J. D., Cave M. D., Crawford J. T., Dale J. W., Eisenach K. D., Gicquel B., Hermans P., Martin C., McAdam R., Shinnick T. M. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol. 1993 Feb;31(2):406–409. doi: 10.1128/jcm.31.2.406-409.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES