Abstract
The performances of three blood culture systems, Hemoline performance diphasic medium (bioMérieux, Marcy l'Etoile, France), Bactec Plus Aerobic/F* (Becton Dickinson, Paramus, N.J.), and Vital Aer (bioMérieux), were compared for the diagnosis of 17 cases of brucellosis. By using a 5-day incubation protocol, positive results were 52.9, 82.4, and 11.8%, respectively. When the protocol was extended to 7 days, the results were 76.5, 94.1, and 47.1%, respectively. Bactec was the fastest system (P < 0.05).
Full Text
The Full Text of this article is available as a PDF (107.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barham W. B., Church P., Brown J. E., Paparello S. Misidentification of Brucella species with use of rapid bacterial identification systems. Clin Infect Dis. 1993 Dec;17(6):1068–1069. doi: 10.1093/clinids/17.6.1068. [DOI] [PubMed] [Google Scholar]
- Casas J., Partal Y., Llosá J., Leiva J., Navarro J. M., de la Rosa M. Detección de Brucella por un sistema automático de hemocultivos: Bact/Alert. Enferm Infecc Microbiol Clin. 1994 Dec;12(10):497–500. [PubMed] [Google Scholar]
- Chomel B. B., DeBess E. E., Mangiamele D. M., Reilly K. F., Farver T. B., Sun R. K., Barrett L. R. Changing trends in the epidemiology of human brucellosis in California from 1973 to 1992: a shift toward foodborne transmission. J Infect Dis. 1994 Nov;170(5):1216–1223. doi: 10.1093/infdis/170.5.1216. [DOI] [PubMed] [Google Scholar]
- Gamazo C., Vitas A. I., López-Goñi I., Díaz R., Moriyón I. Factors affecting detection of Brucella melitensis by BACTEC NR730, a nonradiometric system for hemocultures. J Clin Microbiol. 1993 Dec;31(12):3200–3203. doi: 10.1128/jcm.31.12.3200-3203.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hardy D. J., Hulbert B. B., Migneault P. C. Time to detection of positive BacT/Alert blood cultures and lack of need for routine subculture of 5- to 7-day negative cultures. J Clin Microbiol. 1992 Oct;30(10):2743–2745. doi: 10.1128/jcm.30.10.2743-2745.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lysko P. G., Morse S. A. Neisseria gonorrhoeae cell envelope: permeability to hydrophobic molecules. J Bacteriol. 1981 Feb;145(2):946–952. doi: 10.1128/jb.145.2.946-952.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marchandin H., Compan B., Simeon De Buochberg M., Despaux E., Perez C. Detection kinetics for positive blood culture bottles by using the VITAL automated system. J Clin Microbiol. 1995 Aug;33(8):2098–2101. doi: 10.1128/jcm.33.8.2098-2101.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peiris V., Fraser S., Fairhurst M., Weston D., Kaczmarski E. Laboratory diagnosis of brucella infection: some pitfalls. Lancet. 1992 Jun 6;339(8806):1415–1416. doi: 10.1016/0140-6736(92)91234-y. [DOI] [PubMed] [Google Scholar]
- RUIZ CASTANEDA M. Laboratory diagnosis of brucellosis in man. Bull World Health Organ. 1961;24:73–84. [PMC free article] [PubMed] [Google Scholar]
- Rodríguez A., Abad R., Orduña A. Especies y biovars del genero Brucella. Etiología de la brucelosis humana en España. Enferm Infecc Microbiol Clin. 1992 Jan;10(1):43–48. [PubMed] [Google Scholar]
- Solomon H. M., Jackson D. Rapid diagnosis of Brucella melitensis in blood: some operational characteristics of the BACT/ALERT. J Clin Microbiol. 1992 Jan;30(1):222–224. doi: 10.1128/jcm.30.1.222-224.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yagupsky P. Detection of Brucella melitensis by BACTEC NR660 blood culture system. J Clin Microbiol. 1994 Aug;32(8):1899–1901. doi: 10.1128/jcm.32.8.1899-1901.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zimmerman S. J., Gillikin S., Sofat N., Bartholomew W. R., Amsterdam D. Case report and seeded blood culture study of Brucella bacteremia. J Clin Microbiol. 1990 Sep;28(9):2139–2141. doi: 10.1128/jcm.28.9.2139-2141.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]