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. 1997 Oct;35(10):2665–2669. doi: 10.1128/jcm.35.10.2665-2669.1997

Development of a method for direct quantification of cytomegalovirus antigenemia by flow cytometry.

B M Imbert-Marcille 1, N Robillard 1, A S Poirier 1, M Coste-Burel 1, D Cantarovich 1, N Milpied 1, S Billaudel 1
PMCID: PMC230033  PMID: 9316930

Abstract

Cytomegalovirus (CMV) antigenemia was directly detected in polymorphonuclear leukocytes (PMNLs) from transplant recipients by using flow cytometry (FC). Two fixation and permeabilization methods and seven anti-CMV monoclonal antibodies (MAbs) were evaluated. 1C3, SL20, and NEA-9221 MAbs were more efficacious. The antigenemia detection threshold of FC was 0.05% positive PMNLs, and percentages correlated well with DNA viral load and the appearance of clinical symptoms.

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Selected References

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