Abstract
The reliability of the ESP Culture System II (ESP II; AccuMed International, Westlake, Ohio), a continuously monitoring, nonradiometric mycobacterial culture system, for recovery of mycobacteria from sediments of blood collected in an Isolator tube was evaluated by comparing its performance to inoculation of the sediment onto Middlebrook 7H11/7H11 selective biplates. Of 1,704 blood specimens, 73 (4.3%) were positive for mycobacteria (68 Mycobacterium avium complex and 5 M. tuberculosis). Fifty-three specimens were positive by both methods; 13 were positive by ESP II only, and 7 were positive by Middlebrook agar only (chi square = 1.8; P > 0.05). The mean times to positivity were 15.6 days for ESP II and 19.0 days for Middlebrook agar (P < 0.01). The time to detection was the same for 13 specimens; ESP II was positive first for 33, and agar plates were positive first for 7. ESP II allowed recovery of more mycobacteria (90.4% of all isolates versus 82.2% for Middlebrook agar) from sediments of blood specimens collected in Isolator tubes, and it provided significantly faster detection than did Middlebrook plates.
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- Agy M. B., Wallis C. K., Plorde J. J., Carlson L. C., Coyle M. B. Evaluation of four mycobacterial blood culture media: BACTEC 13A, Isolator/BACTEC 12B, Isolator/Middlebrook agar, and a biphasic medium. Diagn Microbiol Infect Dis. 1989 Jul-Aug;12(4):303–308. doi: 10.1016/0732-8893(89)90094-1. [DOI] [PubMed] [Google Scholar]
- Doern G. V., Westerling J. A. Optimum recovery of Mycobacterium avium complex from blood specimens of human immunodeficiency virus-positive patients by using small volumes of isolator concentrate inoculated into BACTEC 12B bottles. J Clin Microbiol. 1994 Oct;32(10):2576–2577. doi: 10.1128/jcm.32.10.2576-2577.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hanna B. A., Walters S. B., Bonk S. J., Tick L. J. Recovery of mycobacteria from blood in mycobacteria growth indicator tube and Lowenstein-Jensen slant after lysis-centrifugation. J Clin Microbiol. 1995 Dec;33(12):3315–3316. doi: 10.1128/jcm.33.12.3315-3316.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Inderlied C. B., Kemper C. A., Bermudez L. E. The Mycobacterium avium complex. Clin Microbiol Rev. 1993 Jul;6(3):266–310. doi: 10.1128/cmr.6.3.266. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kiehn T. E., Cammarata R. Comparative recoveries of Mycobacterium avium-M. intracellulare from isolator lysis-centrifugation and BACTEC 13A blood culture systems. J Clin Microbiol. 1988 Apr;26(4):760–761. doi: 10.1128/jcm.26.4.760-761.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Labombardi V. J., Carter L., Massarella S. Use of nucleic acid probes to identify mycobacteria directly from Difco ESP-Myco bottles. J Clin Microbiol. 1997 Apr;35(4):1002–1004. doi: 10.1128/jcm.35.4.1002-1004.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Masur H. Recommendations on prophylaxis and therapy for disseminated Mycobacterium avium complex disease in patients infected with the human immunodeficiency virus. Public Health Service Task Force on Prophylaxis and Therapy for Mycobacterium avium Complex. N Engl J Med. 1993 Sep 16;329(12):898–904. doi: 10.1056/NEJM199309163291228. [DOI] [PubMed] [Google Scholar]
- Salfinger M., Stool E. W., Piot D., Heifets L. Comparison of three methods for recovery of Mycobacterium avium complex from blood specimens. J Clin Microbiol. 1988 Jun;26(6):1225–1226. doi: 10.1128/jcm.26.6.1225-1226.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wasilauskas B., Morrell R., Jr Inhibitory effect of the Isolator blood culture system on growth of Mycobacterium avium-M. intracellulare in BACTEC 12B bottles. J Clin Microbiol. 1994 Mar;32(3):654–657. doi: 10.1128/jcm.32.3.654-657.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Witebsky F. G., Keiser J. F., Conville P. S., Bryan R., Park C. H., Walker R., Siddiqi S. H. Comparison of BACTEC 13A medium and Du Pont isolator for detection of mycobacteremia. J Clin Microbiol. 1988 Aug;26(8):1501–1505. doi: 10.1128/jcm.26.8.1501-1505.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woods G. L., Fish G., Plaunt M., Murphy T. Clinical evaluation of difco ESP culture system II for growth and detection of mycobacteria. J Clin Microbiol. 1997 Jan;35(1):121–124. doi: 10.1128/jcm.35.1.121-124.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]