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. 1997 Oct;35(10):2689–2691. doi: 10.1128/jcm.35.10.2689-2691.1997

PCR-enzyme-linked immunosorbent assay and sequencing as an alternative to serology for M-antigen typing of Streptococcus pyogenes.

N A Saunders 1, G Hallas 1, E T Gaworzewska 1, L Metherell 1, A Efstratiou 1, J V Hookey 1, R C George 1
PMCID: PMC230041  PMID: 9316938

Abstract

A rapid PCR-enzyme-linked immunosorbent assay for identification of 10 important emm gene types of Streptococcus pyogenes was developed. The emm genotypes of a coded panel of strains of known M serotype were determined, and in 144 of 149 cases (97%) the results were congruous. Strains of types that were not included in the panel of capture probes were emm genotyped by sequencing.

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Selected References

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