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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1997 Oct;35(10):2692–2694. doi: 10.1128/jcm.35.10.2692-2694.1997

Evaluation of the AMPLICOR CMV test for direct detection of cytomegalovirus in plasma specimens.

M Hiyoshi 1, S Tagawa 1, T Takubo 1, K Tanaka 1, T Nakao 1, Y Higeno 1, K Tamura 1, M Shimaoka 1, A Fujii 1, M Higashihata 1, Y Yasui 1, T Kim 1, A Hiraoka 1, N Tatsumi 1
PMCID: PMC230042  PMID: 9316939

Abstract

We evaluated the AMPLICOR CMV test (PCR) for the direct detection of cytomegalovirus in plasma. Sixty-eight specimens were involved for the comparison between the AMPLICOR test and the antigenemia assay. The sensitivities, specificities, and positive and negative predictive values were 97.1, 100, 100, and 97.1%, respectively, for the AMPLICOR test and 79.4, 100, 100, and 82.9%, respectively, for the antigenemia assay.

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Selected References

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  1. Brytting M., Xu W., Wahren B., Sundqvist V. A. Cytomegalovirus DNA detection in sera from patients with active cytomegalovirus infections. J Clin Microbiol. 1992 Aug;30(8):1937–1941. doi: 10.1128/jcm.30.8.1937-1941.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Freymuth F., Gennetay E., Petitjean J., Eugene G., Hurault de Ligny B., Ryckelynck J. P., Legoff C., Hazera P., Bazin C. Comparison of nested PCR for detection of DNA in plasma with pp65 leukocytic antigenemia procedure for diagnosis of human cytomegalovirus infection. J Clin Microbiol. 1994 Jun;32(6):1614–1618. doi: 10.1128/jcm.32.6.1614-1618.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gondo H., Minematsu T., Harada M., Akashi K., Hayashi S., Taniguchi S., Yamasaki K., Shibuya T., Takamatsu Y., Teshima T. Cytomegalovirus (CMV) antigenaemia for rapid diagnosis and monitoring of CMV-associated disease after bone marrow transplantation. Br J Haematol. 1994 Jan;86(1):130–137. doi: 10.1111/j.1365-2141.1994.tb03263.x. [DOI] [PubMed] [Google Scholar]
  4. Grefte J. M., van der Gun B. T., Schmolke S., van der Giessen M., van Son W. J., Plachter B., Jahn G., The T. H. Cytomegalovirus antigenemia assay: identification of the viral antigen as the lower matrix protein pp65. J Infect Dis. 1992 Sep;166(3):683–684. doi: 10.1093/infdis/166.3.683. [DOI] [PubMed] [Google Scholar]
  5. Ishigaki S., Takeda M., Kura T., Ban N., Saitoh T., Sakamaki S., Watanabe N., Kohgo Y., Niitsu Y. Cytomegalovirus DNA in the sera of patients with cytomegalovirus pneumonia. Br J Haematol. 1991 Oct;79(2):198–204. doi: 10.1111/j.1365-2141.1991.tb04522.x. [DOI] [PubMed] [Google Scholar]
  6. Landry M. L., Ferguson D., Stevens-Ayers T., de Jonge M. W., Boeckh M. Evaluation of CMV Brite kit for detection of cytomegalovirus pp65 antigenemia in peripheral blood leukocytes by immunofluorescence. J Clin Microbiol. 1996 May;34(5):1337–1339. doi: 10.1128/jcm.34.5.1337-1339.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Masuho Y., Matsumoto Y., Sugano T., Fujinaga S., Minamishima Y. Human monoclonal antibodies neutralizing human cytomegalovirus. J Gen Virol. 1987 May;68(Pt 5):1457–1461. doi: 10.1099/0022-1317-68-5-1457. [DOI] [PubMed] [Google Scholar]
  8. Patel R., Smith T. F., Espy M., Wiesner R. H., Krom R. A., Portela D., Paya C. V. Detection of cytomegalovirus DNA in sera of liver transplant recipients. J Clin Microbiol. 1994 Jun;32(6):1431–1434. doi: 10.1128/jcm.32.6.1431-1434.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Spector S. A., Merrill R., Wolf D., Dankner W. M. Detection of human cytomegalovirus in plasma of AIDS patients during acute visceral disease by DNA amplification. J Clin Microbiol. 1992 Sep;30(9):2359–2365. doi: 10.1128/jcm.30.9.2359-2365.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. The T. H., van der Bij W., van den Berg A. P., van der Giessen M., Weits J., Sprenger H. G., van Son W. J. Cytomegalovirus antigenemia. Rev Infect Dis. 1990 Sep-Oct;12 (Suppl 7):S734–S744. [PubMed] [Google Scholar]
  11. Zipeto D., Morris S., Hong C., Dowling A., Wolitz R., Merigan T. C., Rasmussen L. Human cytomegalovirus (CMV) DNA in plasma reflects quantity of CMV DNA present in leukocytes. J Clin Microbiol. 1995 Oct;33(10):2607–2611. doi: 10.1128/jcm.33.10.2607-2611.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. van der Bij W., Schirm J., Torensma R., van Son W. J., Tegzess A. M., The T. H. Comparison between viremia and antigenemia for detection of cytomegalovirus in blood. J Clin Microbiol. 1988 Dec;26(12):2531–2535. doi: 10.1128/jcm.26.12.2531-2535.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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