Abstract
We have developed a technique based on the use of monodisperse magnetic beads to isolate Mycobacterium ulcerans from heterogenous mixtures, prior to PCR amplification. Using this method, we were able to detect M. ulcerans in water samples taken from Phillip Island, Australia, the site of several outbreaks of M. ulcerans disease in recent times.
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- Boondeekhun H. S., Gurtler V., Odd M. L., Wilson V. A., Mayall B. C. Detection of Clostridium difficile enterotoxin gene in clinical specimens by the polymerase chain reaction. J Med Microbiol. 1993 May;38(5):384–387. doi: 10.1099/00222615-38-5-384. [DOI] [PubMed] [Google Scholar]
- Enroth H., Engstrand L. Immunomagnetic separation and PCR for detection of Helicobacter pylori in water and stool specimens. J Clin Microbiol. 1995 Aug;33(8):2162–2165. doi: 10.1128/jcm.33.8.2162-2165.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Flood P., Street A., O'Brien P., Hayman J. Mycobacterium ulcerans infection on Phillip Island, Victoria. Med J Aust. 1994 Feb 7;160(3):160–160. [PubMed] [Google Scholar]
- Kapperud G., Vardund T., Skjerve E., Hornes E., Michaelsen T. E. Detection of pathogenic Yersinia enterocolitica in foods and water by immunomagnetic separation, nested polymerase chain reactions, and colorimetric detection of amplified DNA. Appl Environ Microbiol. 1993 Sep;59(9):2938–2944. doi: 10.1128/aem.59.9.2938-2944.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Muir P., Nicholson F., Jhetam M., Neogi S., Banatvala J. E. Rapid diagnosis of enterovirus infection by magnetic bead extraction and polymerase chain reaction detection of enterovirus RNA in clinical specimens. J Clin Microbiol. 1993 Jan;31(1):31–38. doi: 10.1128/jcm.31.1.31-38.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Olsvik O., Popovic T., Skjerve E., Cudjoe K. S., Hornes E., Ugelstad J., Uhlén M. Magnetic separation techniques in diagnostic microbiology. Clin Microbiol Rev. 1994 Jan;7(1):43–54. doi: 10.1128/cmr.7.1.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilde J., Eiden J., Yolken R. Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions. J Clin Microbiol. 1990 Jun;28(6):1300–1307. doi: 10.1128/jcm.28.6.1300-1307.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]