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. 1997 Nov;35(11):2728–2732. doi: 10.1128/jcm.35.11.2728-2732.1997

Evaluation of three commercial enzyme-linked immunosorbent assays and two latex agglutination assays for diagnosis of primary Epstein-Barr virus infection.

A Svahn 1, M Magnusson 1, L Jägdahl 1, L Schloss 1, G Kahlmeter 1, A Linde 1
PMCID: PMC230050  PMID: 9350722

Abstract

Three commercially available enzyme-linked immunosorbent assays (ELISAs) from Gull, Biotest, and Behring (Enzygnost) and two latex agglutination tests for heterophile antibodies (Monolatex [Biotest] and Mono-Lex [Trinity Laboratories]) were evaluated for the diagnosis of primary Epstein-Barr virus (EBV) infection and EBV seropositivity. Two hundred fourteen consecutive samples from 197 patients with symptoms of primary EBV infection were analyzed by the five assays at a clinical microbiology laboratory. The samples were also analyzed independently by immunofluorescence methods at a reference laboratory. According to the reference methods, 37 patients (40 serum samples) had primary EBV infections, 120 patients (127 serum samples) had had past EBV infections, 33 patients (36 serum samples) were seronegative, and 7 patients (11 serum samples) exhibited atypical reactions. The respective sensitivities and specificities for the diagnosis of primary EBV infection were 95 and 100% for the Gull assays, 100 and 94% for the Biotest assays, and 100 and 89%, for the Enzygnost assays. The Monolatex and Mono-Lex methods showed similar sensitivities and specificities (78 to 85% and 100 to 99%, respectively) for the diagnosis of primary EBV infection. This study demonstrates the usefulness of commercially available assays for the rapid diagnosis of primary EBV infection, but also the importance of large-scale testing of routine samples before choosing an assay.

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Selected References

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  1. Dopatka H. D., Schuy W. Compact Epstein-Barr virus diagnosis based on a defined antigen mix and specific IgA. Res Virol. 1996 Jan-Feb;147(1):53–66. doi: 10.1016/0923-2516(96)80240-8. [DOI] [PubMed] [Google Scholar]
  2. Fleisher G., Lennette E. T., Henle G., Henle W. Incidence of heterophil antibody responses in children with infectious mononucleosis. J Pediatr. 1979 May;94(5):723–728. doi: 10.1016/s0022-3476(79)80138-9. [DOI] [PubMed] [Google Scholar]
  3. Färber I., Wutzler P., Wohlrabe P., Wolf H., Hinderer W., Sonneborn H. H. Serological diagnosis of infectious mononucleosis using three anti-Epstein-Barr virus recombinant ELISAs. J Virol Methods. 1993 May;42(2-3):301–307. doi: 10.1016/0166-0934(93)90041-o. [DOI] [PubMed] [Google Scholar]
  4. Gorgievski-Hrisoho M., Hinderer W., Nebel-Schickel H., Horn J., Vornhagen R., Sonneborn H. H., Wolf H., Siegl G. Serodiagnosis of infectious mononucleosis by using recombinant Epstein-Barr virus antigens and enzyme-linked immunosorbent assay technology. J Clin Microbiol. 1990 Oct;28(10):2305–2311. doi: 10.1128/jcm.28.10.2305-2311.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gray J. J., Caldwell J., Sillis M. The rapid serological diagnosis of infectious mononucleosis. J Infect. 1992 Jul;25(1):39–46. doi: 10.1016/0163-4453(92)93465-3. [DOI] [PubMed] [Google Scholar]
  6. Henle W., Henle G. E., Horwitz C. A. Epstein-Barr virus specific diagnostic tests in infectious mononucleosis. Hum Pathol. 1974 Sep;5(5):551–565. doi: 10.1016/s0046-8177(74)80006-7. [DOI] [PubMed] [Google Scholar]
  7. Henle W., Henle G., Andersson J., Ernberg I., Klein G., Horwitz C. A., Marklund G., Rymo L., Wellinder C., Straus S. E. Antibody responses to Epstein-Barr virus-determined nuclear antigen (EBNA)-1 and EBNA-2 in acute and chronic Epstein-Barr virus infection. Proc Natl Acad Sci U S A. 1987 Jan;84(2):570–574. doi: 10.1073/pnas.84.2.570. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Latif Z. A., Fletcher M. A. Immunochemical studies of infectious mononucleosis--XI. comparison of heterophile antibody inhibitors from the erythrocyte membranes of four mammalian species. Mol Immunol. 1983 Jan;20(1):1–10. doi: 10.1016/0161-5890(83)90099-8. [DOI] [PubMed] [Google Scholar]
  9. Linde A., Andersson J., Lundgren G., Wahren B. Subclass reactivity to Epstein-Barr virus capsid antigen in primary and reactivated EBV infections. J Med Virol. 1987 Feb;21(2):109–121. doi: 10.1002/jmv.1890210203. [DOI] [PubMed] [Google Scholar]
  10. Linde A. Diagnosis of Epstein-Barr virus-related diseases. Scand J Infect Dis Suppl. 1996;100:83–88. [PubMed] [Google Scholar]
  11. Linde A., Kallin B., Dillner J., Andersson J., Jägdahl L., Lindvall A., Wahren B. Evaluation of enzyme-linked immunosorbent assays with two synthetic peptides of Epstein-Barr virus for diagnosis of infectious mononucleosis. J Infect Dis. 1990 May;161(5):903–909. doi: 10.1093/infdis/161.5.903. [DOI] [PubMed] [Google Scholar]
  12. Linderholm M., Boman J., Juto P., Linde A. Comparative evaluation of nine kits for rapid diagnosis of infectious mononucleosis and Epstein-Barr virus-specific serology. J Clin Microbiol. 1994 Jan;32(1):259–261. doi: 10.1128/jcm.32.1.259-261.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Okano M., Matsumoto S., Osato T., Sakiyama Y., Thiele G. M., Purtilo D. T. Severe chronic active Epstein-Barr virus infection syndrome. Clin Microbiol Rev. 1991 Jan;4(1):129–135. doi: 10.1128/cmr.4.1.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Okano M., Thiele G. M., Davis J. R., Grierson H. L., Purtilo D. T. Epstein-Barr virus and human diseases: recent advances in diagnosis. Clin Microbiol Rev. 1988 Jul;1(3):300–312. doi: 10.1128/cmr.1.3.300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Reid M. C., Lachs M. S., Feinstein A. R. Use of methodological standards in diagnostic test research. Getting better but still not good. JAMA. 1995 Aug 23;274(8):645–651. [PubMed] [Google Scholar]
  16. Straus S. E., Cohen J. I., Tosato G., Meier J. NIH conference. Epstein-Barr virus infections: biology, pathogenesis, and management. Ann Intern Med. 1993 Jan 1;118(1):45–58. doi: 10.7326/0003-4819-118-1-199301010-00009. [DOI] [PubMed] [Google Scholar]
  17. Vetter V., Kreutzer L., Bauer G. Differentiation of primary from secondary anti-EBNA-1-negative cases by determination of avidity of VCA-IgG. Clin Diagn Virol. 1994 Feb;2(1):29–39. doi: 10.1016/0928-0197(94)90033-7. [DOI] [PubMed] [Google Scholar]
  18. Weber B., Brunner M., Preiser W., Doerr H. W. Evaluation of 11 enzyme immunoassays for the detection of immunoglobulin M antibodies to Epstein-Barr virus. J Virol Methods. 1996 Mar;57(1):87–93. doi: 10.1016/0166-0934(95)01971-5. [DOI] [PubMed] [Google Scholar]
  19. Wiedbrauk D. L., Bassin S. Evaluation of five enzyme immunoassays for detection of immunoglobulin M antibodies to Epstein-Barr virus viral capsid antigens. J Clin Microbiol. 1993 May;31(5):1339–1341. doi: 10.1128/jcm.31.5.1339-1341.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

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